TMT-based quantitative proteomics reveals protein biomarkers from cultured Pacific abalone (Haliotis discus hannai) in different regions

Luan, Yimu; Yang, Dong; Duan, Xuyuan; Wang, Xiuli; Pang, Yanwei; Li, Qingwei; Gou, Meng

doi:10.1016/j.fochx.2022.100355

Cited by 3 publications

(3 citation statements)

References 34 publications

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“…This downregulation was related to altered ribosome biogenesis. Luan et al reported that the reduced protein synthesis by ribosomes impacted the muscle hypertrophy of abalone (Haliotis discus hannai) . The observed upregulation of these protein turnover-related GO terms possibly indicates a reforming trend of muscle protein returning to the original state.…”

Section: Resultsmentioning

confidence: 99%

“…Luan et al reported that the reduced protein synthesis by ribosomes impacted the muscle hypertrophy of abalone (Haliotis discus hannai). 10 The observed upregulation of these protein turnover-related GO terms possibly indicates a reforming trend of muscle protein returning to the original state. This phenomenon might be attributed to the increased expression of ribosomal proteins in crab muscles during transportation.…”

Section: Functional Domain and Subcellular Localizationmentioning

confidence: 99%

“…As a result, analyzing and qualifying proteomes in muscle samples enables researchers to understand underlying molecular mechanisms behind various meat quality traits . Proteomes technology has been used to investigate changes in dysfunctional muscles, the impact of thawing on muscle quality, the biomarkers in different species, and the mechanism of physiological activity . Numerous proteome detection technologies based on high-precision biomass spectrometry and data processing technologies have emerged.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the Alternations in the Muscle Quality of Swimming Crab (Ovalipes punctatus) during Cold-Chain Transportation Using Physicochemical and TMT-Based Quantitative Proteomic Analysis

Du,

Wu,

Liao

et al. 2024

J. Agric. Food Chem.

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Physicochemical properties and protein alterations in Ovalipes punctatus during cold-chain transportation were examined via sensory scores, water-holding capacity (WHC), glucose (GLU) content, catalase (CAT) activity, urea nitrogen (UN) content, and tandem mass tag (TMT)-based proteomic analysis. The results revealed that sensory characteristics and texture of crab muscle deteriorated during transportation. Proteomic analysis revealed 442 and 470 different expressed proteins (DEPs) in crabs after 18 h (FC) and 36 h (DC) of transportation compared with live crabs (LC). Proteins related to muscle structure and amino acid metabolism significantly changed, as evidenced by the decreased WHC and sensory scores of crab muscle. Glycolysis, calcium signaling, and peroxisome pathways were upregulated in the FC/LC comparison, aligning with the changes in GLU content and CAT activity, revealing the stress response of energy metabolism and immune response in crabs during 0−18 h of transportation. The downregulated tricarboxylic acid (TCA) cycle and carcinogenesis-reactive oxygen species pathways were correlated with the decreasing trend in CAT activity, suggesting a gradual retardation in both energy and antioxidant metabolism in crabs during 18−36 h of transportation. Furthermore, the regulated purine nucleoside metabolic and nucleoside diphosphate-related processes, with the increasing changes in UN content, revealed the accumulation of metabolites in crabs.

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Section: Resultsmentioning

confidence: 99%

Section: Functional Domain and Subcellular Localizationmentioning

confidence: 99%