TMT-based quantitative proteomics analysis reveals the differential proteins between fresh and frozen-thawed sperm of yak (Bos grunniens)

Fan, Yilin; Li, Xiaowei; Guo, Yu; Xiao, He; Wang, Yanwen; Zhao, Dan; Ma, Yan; Feng, Xinxin; Zhang, Jiyue; Li, Jian; Zi, Xiang-dong; Xiong, Xianrong; Fu, Wei; Xiong, Yan

doi:10.1016/j.theriogenology.2023.01.024

Cited by 6 publications

(3 citation statements)

References 30 publications

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“…In Ndufs6 −/− mice, bone marrow-derived mesenchymal stem cells exhibited increased intracellular and mitochondrial ROS levels 49 . NDUFS8 was upregulated in frozen-thawed sperm of Bos grunniens in contrast to that in fresh sperm 50 . Therefore, we speculate that decreased intracellular ATP levels are associated with the related DEPs.…”

Section: Discussionmentioning

confidence: 82%

Proteomic analysis of fipronil-induced molecular defects in spermatozoa

Bae,

Kwon

2024

Sci Rep

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The phenylpyrazole insecticide fipronil has wide-ranging applications from agriculture to public health to control undesirable organisms. However, several studies have reported the residual environmental hazards of fipronil and demonstrated its harmful effects even in mammalian reproduction. Therefore, this study was conducted to demonstrate the mode of action of fipronil on mouse spermatozoa. We treated fipronil to spermatozoa and performed comprehensive function evaluations. Moreover, proteomic analyses were conducted to identify the alteration of protein expression levels in spermatozoa. Most of sperm motility and kinematic parameters and intracellular ATP levels were diminished, and the spontaneous acrosome reaction was promoted after treatment with fipronil. Proteomic analyses revealed altered expression levels of 14 proteins after treatment. These proteins have been reported to be associated with sperm-specific pathways, prominently the cytoskeleton of the sperm, “9 + 2” axoneme composition, metabolism, and fertility. Collectively, our results showed that fipronil alters sperm functional-related proteins and therefore influences male fertility. This study elucidates the possible reproductive toxic hazards associated with male infertility through aberrant suppression of sperm proteins.

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Section: Discussionmentioning

confidence: 82%

Proteomic analysis of fipronil-induced molecular defects in spermatozoa

Bae,

Kwon

2024

Sci Rep

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“…Improving the overall quality of yak semen quality will improve yak reproductive performance and is an important way to improve the yield and economic benefits of yaks [20]. TMT-based quantitative proteomics analysis reveals that the protein expression level of SOD1 protein in the frozen group was significantly lower than that in the fresh group, and the protein expression level of NDUFS8 protein was significantly higher in frozen group [21]. Proteins such as OAZ3, GPx4, and GSTM3 whose upregulation leads to reduced motility were upregulated in the spermatozoa of high ERR bulls, the regulation of ACE, a negative regulator of sperm motility was upregulated in both the spermatozoa and seminal plasma of high ERR bulls [22].…”

Section: Discussionmentioning

confidence: 99%

Quantitative proteomics analysis reveals the key proteins related to semen quality in Niangya yaks

Wang,

Liu,

Cao

et al. 2023

Proteome Sci

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Background Proteins related to sperm motility and sperm morphology have an important impact on sperm function such as metabolism, motility and fertilisation etc. An understanding of the key proteins related to semen quality in Niangya yaks would help to provide support for breeding. However, the key proteins that affect semen quality in Niangya yaks remain unclear. Methods Herein, we applied tandem mass tag (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC‒MS/MS) to analyze the expression levels of sperm proteins in groups of high- and low-quality semen from Niangya yaks. And fifteen differentially expressed proteins (DEPs) were randomly selected for expression level validation by parallel reaction monitoring (PRM). Results Of the 2,092 quantified proteins, 280 were identified as DEPs in the high-quality group versus the low-quality group. Gene Ontology (GO) analysis revealed that in terms of biological pathways, the DEPs were mainly involved in metabolic processes, cell transformation processes, and single organism metabolic processes. In terms of cell composition, the DEPs were mainly located in the cell membrane, organelle, molecular complex. In terms of molecular functions, the most abundant functions of the DEPs were catalytic activity, binding activity, transport activity, and enzyme regulation activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the DEPs were mainly involved in the cytokine and cytokine receptor interaction, notch signaling pathway, lysine biosynthesis, renal function-related protein and proteasome pathway. From protein-protein interaction (PPI) analysis of DEPs involved in important pathways, 6 related proteins affecting the semen quality of Niangya yaks were identified. And the results of the PRM and TMT analysis were consistent. Conclusions The differential sperm proteomic analysis of high- and low-quality semen from Niangya yaks, revealed 6 proteins (PSMC5, PSMD8, PSMB3, HSP90AA1, UGP2 and HSPB1), were mainly concentrated in energy production and metabolism, might play important roles in semen quality, which could serve as candidates for the selection and breeding of Niangya yaks.

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“…Sodium dodecyl sulfate polyacrylamide gel electrophoresis (Figure S1) was used to detect the quality of extracted protein according to Fan et al [18]. Briefly, the extracted proteins were mixed with 5× loading buffer and boiled for 5 min.…”

Section: Protein Extractionmentioning

confidence: 99%

Screening of Lactiplantibacillus plantarum 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying

Chen,

Guo,

Ren

et al. 2023

Foods

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Adhesion to the intestinal tract provides the foundation for Lactobacillus to exert its benefits. Vacuum freeze-drying (VFD) is currently one of the main processing methods for Lactobacillus products. Therefore, the effects of VFD on the adhesion and survival of Lactiplantibacillus plantarum 67 were investigated in this study. The results show that L. plantarum 67 exhibits remarkable tolerance following successive exposure to simulated saliva, gastric juice and intestinal juice, and also has a strong adhesion ability to Caco-2 cells. The adhesion and survival rates of L. plantarum 67 significantly decreased after VFD in phosphate-buffered saline (PBS), whereas they significantly increased in protective agents (PAs) (p < 0.05). Scanning electron microscope observations show that L. plantarum 67 aggregated more to Caco-2 cells in PAs than in PBS, and its shape and size were protected. Proteomics detection findings indicated that differentially expressed proteins (DEPs) related to adhesins and vitality and their pathways in L. plantarum 67 were significantly affected by VFD (p < 0.05). However, the expression of DEPs (such as cold shock protein, cell surface protein, adherence protein, chitin-binding domain and extracellular transglycosylase, membrane-bound protein) was improved by PAs. Compared with PBS, the PAs significantly adjusted the phosphotransferase system and amino sugar and nucleotide sugar metabolism pathways (p < 0.05). VFD decreased the adhesion and vitality of L. plantarum 67, while the PAs could exert protective effects by regulating proteins and pathways related to adhesion and vitality.

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TMT-based quantitative proteomics analysis reveals the differential proteins between fresh and frozen-thawed sperm of yak (Bos grunniens)

Cited by 6 publications

References 30 publications

Proteomic analysis of fipronil-induced molecular defects in spermatozoa

Proteomic analysis of fipronil-induced molecular defects in spermatozoa

Quantitative proteomics analysis reveals the key proteins related to semen quality in Niangya yaks

Screening of Lactiplantibacillus plantarum 67 with Strong Adhesion to Caco-2 Cells and the Effects of Protective Agents on Its Adhesion Ability during Vacuum Freeze Drying

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