TMT-based quantitative proteomic analysis of porcine muscle associated with postmortem meat quality

Hou, Xinhua; Liu, Qiufeng; Meng, Qingshi; Wang, Ligang; Hua, Yan; Zhang, Longchao; Wang, Lixian

doi:10.1016/j.foodchem.2020.127133

Cited by 56 publications

(35 citation statements)

References 42 publications

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“…Since there was no difference in fiber types in breast muscle, the higher shear force and lower pressing loss in SG meat than in FG meat may be attributed to the larger muscle fibers with less extracellular space in the former. Similar results were obtained for pork; the muscle fibers of meat with a high drip loss were much smaller and less closely aligned than meat with a low drip loss ( Hou et al, 2020 ). People in China tend to judge poultry products with high pigments as prolonged raising cycle meat with high nutrient levels.…”

Section: Discussionsupporting

confidence: 76%

Fiber characteristics and meat quality of different muscular tissues from slow- and fast-growing broilers

Weng

Huo

et al. 2022

Poultry Science

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Chicken meat is an important source of high-quality animal protein. Its consumption continues to grow in both developed and developing countries. Muscle fiber characteristics are key determinants of meat quality and quantity. Skeletal muscle is a highly plastic tissue that is affected by breed differences and muscular tissues. However, studies regarding the effects of different breeds and muscular tissues on the fibers and meat quality traits in broilers are lacking. In this study, Ross 308 chickens (fast-growing [ FG ] broilers) and Xueshan chickens (slow-growing [ SG ] broilers) were selected, and their fiber and meat quality traits were characterized. The results showed that the breast muscle primarily comprised glycolytic fibers, whereas the leg muscle comprised glycolytic and a few oxidative fibers, regardless of the breed. The highest percentage of oxidative fibers (26.51%) appeared in the soleus muscle ( SOL ) of SG broilers. In addition, higher shear force, lower pressing loss, and thicker muscle fibers with less extracellular space were observed for SG meat than for FG meat. When comparing the different muscular tissues, a higher oxidative fiber percentage, ultimate pH, redness, and intramuscular fat ( IMF ) content were detected in the leg muscle than in the breast muscle in the 2 breeds. In summary, these data indicated that SG broilers had thicker muscle fibers than the FG broilers and that the leg muscle had more oxidative fibers than the breast muscle. Thicker fibers may contribute to increased firmness and more oxidative fibers lead to higher redness value and IMF content.

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Section: Discussionsupporting

confidence: 76%

Fiber characteristics and meat quality of different muscular tissues from slow- and fast-growing broilers

Weng

Huo

et al. 2022

Poultry Science

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“…The quantitative analysis of proteomic protein was conducted using TMT labeling method. The protein digestion was performed as described by Hou et al [ 48 ]. Briefly, dithiothreitol was firstly added into 100 μg of extracted protein with 25 mM final concentration and incubated at 37 °C for 1 h. Then, iodoacetamide with 50 mM final concentration was added and incubated in darkness at room temperature for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…The labeled samples were pooled in equal proportion and subjected to nano-liquid chromatography–tandem mass spectrometry (nanoLC–MS/MS). NanoLC–MS/MS analysis was performed on an Orbitrap Fusion Tribrid MS (Thermo, Waltham, MA) equipped with an UltiMate 3000 system according to the procedure described by Hou et al [ 48 ]. The collected raw MS/MS data were processed using Maxquant proteomics software (Version 1.6.4) against UniProt proteomes of Peniophora genus.…”

Section: Methodsmentioning

confidence: 99%

Selective delignification of poplar wood with a newly isolated white-rot basidiomycete Peniophora incarnata T-7 by submerged fermentation to enhance saccharification

Yue

et al. 2021

Biotechnol Biofuels

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Background Pretreatment is a critical step required for efficient conversion of woody biomass into biofuels and platform chemicals. Fungal pretreatment is regarded as one of the most promising technology for woody biomass conversion but remains challenging for industrial application. The exploration of potential fungus strain with high efficient delignification and less processing time for woody biomass pretreatment will be valuable for development of biorefinery industry. Here, a newly isolated white-rot basidiomycete Peniophora incarnate T-7 was employed for poplar wood pretreatment. Results The chemical component analysis showed that cellulose, hemicellulose and lignin from poplar wood declined by 16%, 48% and 70%, respectively, after 7 days submerged fermentation by P. incarnate T-7. Enzymatic saccharification analysis revealed that the maximum yields of glucose and xylose from 7 days of P. incarnate T-7 treated poplar wood reached 33.4% and 27.6%, respectively, both of which were enhanced by sevenfold relative to the untreated group. Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), X-ray diffraction (XRD) and pyrolysis gas chromatography–mass spectrometry (Py-GC/MS) characterization confirmed that lignocellulosic structure of poplar wood was largely broken by P. incarnate T-7, including delignification and de-crystalline of cellulose. Meanwhile, lignin component of poplar wood was selectively degraded by P. incarnate T-7, and G-type unit of lignin was preferentially attacked by the strain. Furthermore, quantitative proteomic analysis revealed that a considerable amount of lignocellulolytic enzymes were detected in the secretory proteins of P. incarnate T-7, especially with high abundance of lignin-degrading enzymes and hemicellulases. Combination of quantitative proteomic with transcriptomic analysis results showed that most of those lignocellulolytic enzymes were highly upregulated on poplar wood substrate compared to glucose substrate. Conclusions This study showed that P. incarnate T-7 could selectively delignify poplar wood by submerged fermentation with short time of 7 days, which greatly improved its enzymatic saccharification efficiency. Our results suggested that P. incarnate T-7 might be a promising candidate for industrial woody biomass pretreatment.

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“…(ii) TMTpro labeling. Two sets of TMTpro Plex amine-reactive reagents were used to label 30 samples ( 46 ). Channel 126 was used to label an equally proportioned sample as the reference channel.…”

Section: Methodsmentioning

confidence: 99%

Single-Cell Transcriptome Sequencing and Proteomics Reveal Neonatal Ileum Dynamic Developmental Potentials

et al. 2021

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We found previously unknown neonatal ileum developmental potentials: specific increases in undifferentiated cells, unique enterocyte differentiation, and time dependent reduction in secretory cells. Specific transcriptional factors (TFs), ligand-receptor pairs, G protein-coupled receptors, transforming growth factor β, bone morphogenetic protein signaling pathways, and the gut mucosal microbiota are involved in this process.

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TMT-based quantitative proteomic analysis of porcine muscle associated with postmortem meat quality

Cited by 56 publications

References 42 publications

Fiber characteristics and meat quality of different muscular tissues from slow- and fast-growing broilers

Fiber characteristics and meat quality of different muscular tissues from slow- and fast-growing broilers

Selective delignification of poplar wood with a newly isolated white-rot basidiomycete Peniophora incarnata T-7 by submerged fermentation to enhance saccharification

Single-Cell Transcriptome Sequencing and Proteomics Reveal Neonatal Ileum Dynamic Developmental Potentials

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