TMT-based proteomics analysis reveals the protective effects of Xuefu Zhuyu decoction in a rat model of traumatic brain injury

Zhou, Dan; Liu, Jiamiao; Yang, Hang; Li, Teng; Li, Pengfei; Guo, Shichao; Liu, Tao; Xia, Zian; Wang, Yang

doi:10.1016/j.jep.2020.112826

Cited by 26 publications

(24 citation statements)

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“…Quantitative proteomics is the accurate quantification and identification of all proteins expressed in a genome or all proteins in a complex mixed system [ 18 ]. Due to the advantages of proteomics technology, it has been widely used in the research field of Traditional Chinese Medicine (TCM) to explore bioregulatory mechanisms and has provided valuable information on biological mechanisms [ 19 , 20 ]. Isobaric tags for relative and absolute quantitation (iTRAQ) is an isobaric labeling method.…”

Section: Introductionmentioning

confidence: 99%

Tandem mass tag-based proteomics analysis of type 2 diabetes mellitus with non-alcoholic fatty liver disease in mice treated with acupuncture

Wang

et al. 2022

Bioscience Reports

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Objective: To explore the proteomics profiles of hepatocytes of mice treated with acupuncture for type 2 diabetes mellitus (T2DM) with non-alcoholic fatty liver disease (NAFLD). Methods: We used a Tandem mass tag (TMT)-based quantitative proteomics approach to identify proteins with potential molecular mechanisms associated with acupuncture interventions for T2DM with NAFLD. Results: Acupuncture effectively improved body weight, blood glucose, and insulin levels in T2DM with NAFLD mouse models and reversed steatosis within hepatocytes. Quantitative TMT- based proteomics analysis identified a total of 4710 quantifiable proteins and 1226 differentially expressed proteins (DEPs) in the model control group (MCG) compared to the normal control group (NCG). The Acupuncture Treatment Group (ATG) presented in 122 DEPs compared to the MCG group. We performed a bioinformatics analysis, which revealed that DEPs enriched in the KEGG pathway after acupuncture treatment were mainly involved in the PPAR signalling pathway, fatty acid biosynthesis, fatty acid metabolism, fatty acid elongation, fat digestion, and absorption. We used parallel reaction monitoring (PRM) technology to explore the association of aldehyde oxidase 1 (Aox1), acyl-coenzyme A thioesterase 2 (Acot2), perilipin-2 (Plin2), acetyl-CoA carboxylase 1 (Acc), NADP-dependent malic enzyme (Me1), fatty acid synthase (Fasn), ATP-citrate synthase (Acly), fatty acid binding protein (Fabp2) with lipid synthesis, fatty acid oxidation and hepatocyte steatosis. Conclusions: Our results show that acupuncture can regulate the protein expression of T2DM in the NAFLD mice model, and can effectively improve hepatocyte steatosis, and has potential benefits for the clinical treatment of this disease.

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Section: Introductionmentioning

confidence: 99%

Tandem mass tag-based proteomics analysis of type 2 diabetes mellitus with non-alcoholic fatty liver disease in mice treated with acupuncture

Wang

et al. 2022

Bioscience Reports

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“…All craniotomies were created midway between the bregma and the lambda sutures in the left hemisphere of the skull. [5] 2.3 | Cell culture BV-2 microglial cells were plated at a density of 10 6 cells/well in sixwell plates and incubated at 37°C in a humidified 5% CO 2 incubator.…”

Section: Controlled Cortical Impact (Cci) Model Of Tbimentioning

confidence: 99%

“…The same weight (150 μg) tissue of the traumatic penumbra area and similar areas of sham-TBI mice (Figure 4A) tissue were lysed as previously described. [5] Equal amounts of the lysate (25 μg) were separated on denaturing sodium dodecyl sulfate/polyacrylamide gels and transferred onto nitrocellulose membranes. Membranes were blocked in 5% nonfat, dried milk in TBST (50 mM Tris, pH 7.5, 0.15 M NaCl, 0.05% Tween-20) at room temperature (RT) for 1 h, and then incubated with primary antibody (NLRP3: 1:1000, ASC: 1:1000, Santa Cruz Biotechnology; Caspase-1: 1:1000, Abcam; Myd88: 1:1000, p-ERK: 1:1000, ERK: 1:1000, p-NF-κbp65:…”

Section: Western Blot Analysismentioning

confidence: 99%

“…Gene expression relative to the control group or sham group was calculated using the ddCT method. [5]…”

Section: Relative Quantitative Real-time Polymerase Chain Reaction Analysismentioning

confidence: 99%

“…[3] Studies have proved that microglia play a key role in secondary injury via the synthesis and secretion of many different inflammatory cytokines and chemokines after TBI. [4,5] So, how to effectively regulate the microglia function to attenuate the secondary injury is a hot topic in neuroscience.…”

Section: Introductionmentioning

confidence: 99%

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Sodium houttuyfonate attenuates neurological defects after traumatic brain injury in mice via inhibiting NLRP3 inflammasomes

Yao

Wang

Chen

et al. 2021

J Biochem & Molecular Tox

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Sodium houttuyfonate (SH) is a chemical compound synthesized by houttuynin and sodium bisulfite. As it has antinflammatory effects, SH has been widely used to treat autoimmune diseases, including post events following traumatic brain injury (TBI).Meanwhile, NOD-like receptor with pyrin domain containing-3 (NLRP3) inflammasomes in microglia may play a central role in TBI. But to date, the intracellular mechanisms involved in the anti-inflammatory effects of SH in TBI remain unknown, especially whether regulating NLRP3. To gain an insight into this possibility, we conducted cell culture and biochemical studies on the effect of SH on NLRP3 inflammasome in microglia. The results showed that SH inhibited TLR4 and NLRP3 inflammasome activation in the microglia cell. In parallel, phosphorylation of ERK and NF-κB p65, which play a key role in NLRP3 inflammasome formation, was decreased. Intraperitoneal injection of SH into TBI mice significantly reduced the modified neurological severity score (mNSS), as well as the degree of microglia apoptosis post-controlled cortical impact (CCI). Immunohistochemistry, Western blot analysis, and reverse-transcription polymerase chain reaction (RT-PCR) revealed that SH markedly reduced NLRP3 inflammasome activation, TLR4 activity, phosphorylation of ERK and NF-κB. Moreover, SH significantly inhibited microglia activation post-CCI, but effectively promoted the astrocyte activation and angiopoiesis.Taken together, our research provides evidence that SH attenuated neurological deficits post TBI through inhibiting NLRP3 inflammasome activation, via influencing the TLR4/NF-κB signaling pathway. These findings explain the intracellular mechanism of the anti-inflammatory activity caused by SH treatment following TBI.

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A Self‐Gelling Powder Directly Co‐Assembled by Natural Small Molecules for Traumatic Brain Injury

Yang,

Luo,

Song

et al. 2024

Adv Funct Materials

Self Cite

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The prognosis for traumatic brain injury (TBI) depends largely on prompt hemostasis and effective pharmacologic interventions. Natural all‐small‐molecule self‐gelling powder, integrating the advantages of self‐assembled small‐molecule hydrogels and powders, is expected to provide timely and effective prehospital management of TBI. However, the synthesis and application of natural all‐small‐molecule self‐gelling powder is still uncharted territory. In this study, an all‐small‐molecule co‐assembled MGF‐H3BO3‐RUT (MBR) self‐gelling powder is fabricated through the co‐assembly of mangiferin (MGF) and rutin (RUT) in H3BO3/NaOH aqueous solution. Both compounds can bind with boric acid, leading to co‐assembling into hydrogels through hydrogen‐bonding interactions and π–π stacking. MBR self‐gelling powder is then obtained by drying the as‐prepared hydrogels, thus integrating hemostasis and pharmacodynamics into one. Remarkably, it displays robust regeneration capabilities, while retaining excellent self‐healing properties and injectability after drying‐hydration cycles. Moreover, MBR self‐gelling powder not only achieves rapid effective hemostasis but also attenuates conspicuously cerebral edema and inflammatory response after TBI by in situ spraying, exhibiting notable neuroprotective effects without discernible toxic side effects. This study provides a novel assembly strategy and application form for self‐assembled gel materials originating from natural small molecules, offering promising avenues for the treatment of TBI in the acute phase.

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TMT-based proteomics analysis reveals the protective effects of Xuefu Zhuyu decoction in a rat model of traumatic brain injury

Cited by 26 publications

References 73 publications

Tandem mass tag-based proteomics analysis of type 2 diabetes mellitus with non-alcoholic fatty liver disease in mice treated with acupuncture

Tandem mass tag-based proteomics analysis of type 2 diabetes mellitus with non-alcoholic fatty liver disease in mice treated with acupuncture

Sodium houttuyfonate attenuates neurological defects after traumatic brain injury in mice via inhibiting NLRP3 inflammasomes

A Self‐Gelling Powder Directly Co‐Assembled by Natural Small Molecules for Traumatic Brain Injury

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