TMT-based proteomics analysis of the anti-hepatocellular carcinoma effect of combined dihydroartemisinin and sorafenib

Hou, Chunying; Guo, Dongqing; Xue, Yu; Wang, Shuyan; Liu, Tianhua

doi:10.1016/j.biopha.2020.109862

Cited by 22 publications

(12 citation statements)

References 43 publications

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“…The results show that DHA regulates gene expression which is associated with angiogenesis, apoptosis, cell cycle and various pathways (64). As a kind of non-coding RNA, Micro-RNA (miR) plays an important role in tumor by connecting to mRNA to regulate gene expression (72) (58). Further, the metabonomic analysis of DHA in the treatment of esophageal cancer is performed.…”

Section: Other Anti-tumor Effectsmentioning

confidence: 99%

“…In conclusion, DHA has demonstrated significant anti-tumor and inhibition of inflammation effects and is a promising drug with a wide range of targets. In recent years, studies on the genomics (64), proteomics (72,113), metabolomics (58,90) and network pharmacology (43) of DHA therapy have provided a basis for elucidating the specific pharmacological mechanism and targets of DHA, but there are few clinical studies on DHA. Further phase II and III clinical trials are required for DHA.…”

Section: Summary and Future Outlookmentioning

confidence: 99%

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Dihydroartemisinin: A Potential Drug for the Treatment of Malignancies and Inflammatory Diseases

Jin

Fujimoto

2021

Front. Oncol.

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Dihydroartemisinin (DHA) has been globally recognized for its efficacy and safety in the clinical treatment of malaria for decades. Recently, it has been found that DHA inhibits malignant tumor growth and regulates immune system function in addition to anti-malaria. In parasites and tumors, DHA causes severe oxidative stress by inducing excessive reactive oxygen species production. DHA also kills tumor cells by inducing programmed cell death, blocking cell cycle and enhancing anti-tumor immunity. In addition, DHA inhibits inflammation by reducing the inflammatory cells infiltration and suppressing the production of pro-inflammatory cytokines. Further, genomics, proteomics, metabolomics and network pharmacology of DHA therapy provide the basis for elucidating the pharmacological effects of DHA. This review provides a summary of the recent research progress of DHA in anti-tumor, inhibition of inflammatory diseases and the relevant pharmacological mechanisms. With further research of DHA, it is likely that DHA will become an alternative therapy in the clinical treatment of malignant tumors and inflammatory diseases.

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Section: Other Anti-tumor Effectsmentioning

confidence: 99%

Section: Summary and Future Outlookmentioning

confidence: 99%

Dihydroartemisinin: A Potential Drug for the Treatment of Malignancies and Inflammatory Diseases

Jin

Fujimoto

2021

Front. Oncol.

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“…Quantitative proteomics analysis of the anti-HCC efficacy of dihydroartemisinin (DHA) combined with sorafenib may help to understand the related molecular mechanism of anti-HCC. 85 The drug resistance of ovarian cancer cell lines was evaluated with iTRAQ LC-MS/MS, and 28 biomarkers that might lead to cisplatin resistance were identified. 86 Radio resistance biomarkers in several cancers, such as breast cancer, prostate cancer, and lung cancer, were identified with MS-based proteomics approaches.…”

Section: Discovering Drug Resistance Biomarkers With Quantitative Proteomicsmentioning

confidence: 99%

Quantitative proteomics characterization of cancer biomarkers and treatment

Yang

Shi

Zhang

et al. 2021

Molecular Therapy - Oncolytics

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Cancer accounted for 16% of all death worldwide in 2018. Significant progress has been made in understanding tumor occurrence, progression, diagnosis, treatment, and prognosis at the molecular level. However, genomics changes cannot truly reflect the state of protein activity in the body due to the poor correlation between genes and proteins. Quantitative proteomics, capable of quantifying the relatively different protein abundance in cancer patients, has been increasingly adopted in cancer research. Quantitative proteomics has great application potentials, including cancer diagnosis, personalized therapeutic drug selection, real-time therapeutic effects and toxicity evaluation, prognosis and drug resistance evaluation, and new therapeutic target discovery. In this review, the development, testing samples, and detection methods of quantitative proteomics are introduced. The biomarkers identified by quantitative proteomics for clinical diagnosis, prognosis, and drug resistance are reviewed. The challenges and prospects of quantitative proteomics for personalized medicine are also discussed.

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“…10 μg protein samples were added to 5X loading buffer at a rate of 5 : 1 (V/V), and then, the mixture was put in the boiling water for 5 min. e purity of proteins was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on the basis of Hou et al [19].…”

Section: Protein Extraction and Quantificationmentioning

confidence: 99%

TMT-Based Quantitative Proteomics Analysis of the Fish-Borne Spoiler Shewanella putrefaciens Subjected to Cold Stress Using LC-MS/MS

Gao

Mei

et al. 2021

Journal of Chemistry

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Shewanella putrefaciens is a specific spoilage bacterium for fish during cold storage. To better understand the molecular mechanisms of cold stress adaptation of S. putrefaciens, tandem mass tag- (TMT-) based quantitative proteomic analysis was performed to detect the effects of cold stress on protein expression profiles in S. putrefaciens which had been cultivated at 4°C and 30°C, respectively. A total of 266670 peptide spectrum matching numbers were quantified proteins after data analysis. Of the 2292 proteins quantitatively analyzed, a total of 274 were found to be differentially expressed (DE) under cold stress compared with the nonstress control. By integrating the results of Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, 9 common KEGG terms were found notable for the cold-responsive proteins. Generally, the DE proteins involved in carbohydrate, amino acid, and fatty acid biosynthesis and metabolism were significantly upregulated, leading to a specific energy conservation survival mode. The DE proteins related to DNA repair, transcription, and translation were upregulated, implicating change of gene expression and more protein biosynthesis needed in response to cold stress.

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TMT-based proteomics analysis of the anti-hepatocellular carcinoma effect of combined dihydroartemisinin and sorafenib

Cited by 22 publications

References 43 publications

Dihydroartemisinin: A Potential Drug for the Treatment of Malignancies and Inflammatory Diseases

Dihydroartemisinin: A Potential Drug for the Treatment of Malignancies and Inflammatory Diseases

Quantitative proteomics characterization of cancer biomarkers and treatment

TMT-Based Quantitative Proteomics Analysis of the Fish-Borne Spoiler Shewanella putrefaciens Subjected to Cold Stress Using LC-MS/MS

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