TMPO-AS1 promotes cell proliferation of thyroid cancer via sponging miR-498 to modulate TMPO

Abstract

Background: Thyroid cancer (TC) is the most frequent endocrine malignancy. Long noncoding RNAs (lncRNAs) have been confirmed to act as significant roles in tumor development. The role of lncRNA TMPO-AS1 in TC is still unclear, so it remains to be explored. The aim of the research is to investigate the role and regulatory mechanism of TMPO-AS1 in TC. Methods: TMPO-AS1 and TMPO expression in TC tumors and cells was detected by TCGA database and QRT-PCR assay respectively. CCK-8, EDU, TUNEL and western blot assays were conducted to identify the biological functions of TMPO-AS1 in TC. Luciferase reporter and RNA pull down assays were conducted to measure the interaction among TMPO-AS1, TMPO and miR-498. Results: TMPO-AS1 was overexpressed in TC tissues and cell lines. Knockdown of TMPO-AS1 suppressed cell growth and accelerated cell apoptosis in TC. Furthermore, downregulation of TMPO-AS1 suppressed TMPO expression in TC. The data suggested that TMPO expression was upregulated in TC tissues and cell lines and was positively correlated with TMPO-AS1 expression in TC. Furthermore, the expression of miR-498 presented low expression in TC cells. And miR-498 expression was negatively regulated by TMPO-AS1, meanwhile, TMPO expression was negatively regulated by miR-498 in TC cells. Besides, it was confirmed that TMPO-AS1 could bind with miR-498 and TMPO in TC cells. In addition, it was validated that TMPO-AS1 elevated the levels of TMPO via sponging miR-498 in TC cells. Conclusions: TMPO-AS1 promotes cell proliferation in TC via sponging miR-498 to modulate TMPO.

TMPO-AS1 Regulates the Aggressiveness-Associated Traits of Nasopharyngeal Carcinoma Cells Through Sponging miR-320a

“…Increasing evidence has confirmed that lncRNA TMPO-AS1 is upregulated in diverse types of cancers, including triple-negative breast cancer, thyroid cancer, and gastric carcinoma. 22–24 Similarly, in the current study, TMPO-AS1 was discovered to be distinctly upregulated in NPC tissue samples. TMPO-AS1 is reported to act as an essential participant in cancer cells’ growth and metastasis.…”

<p>LncRNA TMPO-AS1 Promotes Proliferation and Invasion by Sponging miR-383-5p in Glioma Cells</p>

“… 7 TMPO-AS1 can promote cell proliferation of thyroid cancer via sponging miR-498. 19 Consistently, after verifying the direct binding condition between TMPO-AS1 and miR-383-5p, a series of cellular function experiments exhibited that inhibition of miR-383-5p could reverse the low activities of glioma cell proliferation, migration and invasion caused by TMPO-AS1 knockdown, indicating that TMPO-AS1 might sponge miR-383-5p to regulate cellular functions. The functionality of miR-383-5p has been characterized in several types of human cancers.…”

“…7 TMPO-AS1 can promote cell proliferation of thyroid cancer via sponging miR-498. 19 Consistently, after verifying the direct binding condition between TMPO-AS1 and miR-383-5p, a series of cellular function experiments exhibited that inhibition of miR-383-5p could reverse the low activities…”

The Long Non-coding RNA TMPO-AS1&nbsp; Promotes Bladder Cancer Growth and Progression via OTUB1-induced E2F1&nbsp;Deubiquitination

“…Numerous studies have revealed that TMPO-AS1 contributes to tumorigenesis. However, most of them suggest that this is mediated via a TMPO-AS1-miRNA-mRNA axis [17,18,51,52], while others propose that TMPO-AS1 forms RNA-RNA complexes to regulate gene expression [19,55]. Very recently, a study has shown that TMPO-AS1 promotes BC cell growth via a TMPO-AS1/miR-98-5p/EBF1 positive feedback loop [51].…”

“…Moreover, recent studies have demonstrated that upregulated TMPO antisense RNA 1 (TMPO-AS1) serves as competing endogenous RNA to sponge microRNAs (miRNAs) in multiple carcinomas including hepatocellular carcinoma, thyroid cancer, lung adenocarcinoma, and breast cancer [17,18,19,20]. However, whether TMPO-AS1 plays a role by interacting with other molecules in BC is an interesting question requiring further investigation.…”