TMK1-mediated auxin signalling regulates differential growth of the apical hook

Cao, Min; Chen, Rong; Li, Pan; Yu, Yongqiang; Zheng, Rui; Ge, Danfeng; Zheng, Wei; Wang, Xuhui; Gu, Yangtao; Gelová, Zuzana; Friml, Jiřı́; Zhang, Heng; Liu, Renyi; He, Jun; Xu, Tongda

doi:10.1038/s41586-019-1069-7

Cited by 181 publications

(226 citation statements)

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“…Consistent with previous reports (Wang et al, 2016), interfering with the auxin receptors TIR1 and AFB2 in tir1, afb2 and tir1 afb2 mutant also led to a significant reduction in root growth in response to temperature, demonstrating that auxin perception is required for this response (Figure 6A). To complement these data, we impaired another branch of auxin signaling by interfering with TMKs function, which are membrane localized receptor like kinases involved in the perception of auxin independently of the TIR/AFB system (Cao et al, 2019;Xu et al, 2014). Like observed with the TIR/AFB related mutants, we found a reduced root elongation in tmk1,4 compared to wild type during the root temperature response ( Figure 6B).…”

Section: Hy5-pif Activity Co-regulate Root Thermomorphogenesissupporting

confidence: 55%

“…The observation that shoot and the root thermomorphogenesis were negatively correlated was intriguing and prompted us to test whether modulating shoot thermo-response was sufficient to impact root growth. To investigate this idea, we used a genetic chimera approach by taking advantage of a HA-YFP-HA-HY5 fusion protein (DOF-HY5) that showed restricted cell-to-cell movement and aimed at driving its expression specifically in the shoot of hy5 mutants using CAB3 or CER6 promoters (Burko et al, 2020b;Procko et al, 2016). In line with previous studies (Chen et al, 2016;Procko et al, 2016), we detected strong accumulation of DOF-HY5 in leaves, petioles and hypocotyls for both constructs, confirming that our CAB3 and CER6 promoters were driving expression in the shoot (Supplementary Figure 3A-F; Burko et al, 2020b).…”

Section: Hy5-pif Activity Co-regulate Root Thermomorphogenesismentioning

confidence: 99%

“…To investigate this idea, we used a genetic chimera approach by taking advantage of a HA-YFP-HA-HY5 fusion protein (DOF-HY5) that showed restricted cell-to-cell movement and aimed at driving its expression specifically in the shoot of hy5 mutants using CAB3 or CER6 promoters (Burko et al, 2020b;Procko et al, 2016). In line with previous studies (Chen et al, 2016;Procko et al, 2016), we detected strong accumulation of DOF-HY5 in leaves, petioles and hypocotyls for both constructs, confirming that our CAB3 and CER6 promoters were driving expression in the shoot (Supplementary Figure 3A-F; Burko et al, 2020b). While we detected DOF-HY5 accumulation in the root of the pCER6:DOF-HY5 line, we did not detect fluorescence signal in the root of the pCAB3:DOF-HY5 lines, indicating that expression driven from the CAB3 promoter was specific to the shoot and that our tagged version of HY5 was not able to move from the shoot to the root ( Figure 4A Figure 4D,E).…”

Section: Hy5-pif Activity Co-regulate Root Thermomorphogenesismentioning

confidence: 99%

“…and pCER6:HA-YFP-HA-HY5 in the hy5 background (Lian et al, 2011) as described in (Burko et al, 2020b).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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Shoot and root thermomorphogenesis are linked by a developmental trade-off

Gaillochet

Burko

Platre

et al. 2020

Preprint

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Temperature is one of the most impactful environmental factors to which plants adjust their growth and development. While the regulation of temperature signaling has been extensively investigated for the aerial part of plants, much less is known and understood about how roots sense and modulate their growth in response to fluctuating temperatures. Here we found that shoot and root growth responses to high ambient temperature are coordinated during early seedling development. A shoot signaling module that includes HY5, the phytochromes and the PIFs exerts a central function in coupling these growth responses and control auxin levels in the root. In addition to the HY5/PIF-dependent shoot module, a regulatory axis composed of auxin biosynthesis and auxin perception factors controls root responses to high ambient temperature. Together, our findings show that shoot and root developmental responses to temperature are tightly coupled during thermomorphogenesis and suggest that roots integrate energy signals with local hormonal inputs.genes including YUC8 Gangappa and Kumar, 2017). Both HY5 and PIF4 expression levels and protein abundance are tightly regulated by a plethora of factors (reviewed in (Lau and Deng, 2012;Quint et al., 2016)). Among those, CONSTITUTIVE PHOTOMORPHOGENESIS PROTEIN1 (COP1) and DEETIOLATED1 (DET1) trigger HY5 degradation and promote both PIF4 expression and protein stabilization (Gangappa and Kumar, 2017;Osterlund et al., 2000;Saijo et al., 2003;Yanagawa et al., 2004). The collective genetic activity of PIF4, HY5, COP1 and DET1 defines an intertwined regulatory module that acts at the interface between light and temperature signalling Gangappa and Kumar, 2017). Interestingly, HY5 protein has also been shown to translocate from the shoot to the root and to coordinate carbon fixation with nitrogen uptake (Chen et al., 2016). Importantly, roots can autonomously sense and respond to temperature (Bellstaedt et al., 2019), which might allow them to reach deeper and cooler layers of the soil under warm surface conditions (Illston and Fiebrich, 2017). However, in contrast to the shoot, the molecular mechanisms underlying plant root thermo-responses have so far remained elusive. Similarly tothe shoot, maintenance of auxin homeostasis is critical for the root response to temperature (Wang et al., 2016). In line with this idea, auxin signaling increases upon perception of higher ambient temperature (Hanzawa et al., 2013;Wang et al., 2016). In this context the auxin efflux transporters PIN2 and PILS6 mediate auxin transport and local accumulation at the root, which in turn triggers developmental response to temperature in the root (Feraru et al., 2019;Hanzawa et al., 2013). Furthermore, the auxin receptors TIR1 and AFB2 are stabilized upon increased ambient temperature by forming a protein complex with HEAT SHOCK PROTEIN 90 (HSP90) and its co-chaperone SUPPRESSOR OF G2 ALLELE SKP1 (SGT1). The accumulation of TIR1 and AFB2 subsequently activates auxin signaling and mediates root thermo-sensory elongation (...

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Section: Hy5-pif Activity Co-regulate Root Thermomorphogenesissupporting

confidence: 55%

Section: Hy5-pif Activity Co-regulate Root Thermomorphogenesismentioning

confidence: 99%

Section: Hy5-pif Activity Co-regulate Root Thermomorphogenesismentioning

confidence: 99%

“…and pCER6:HA-YFP-HA-HY5 in the hy5 background (Lian et al, 2011) as described in (Burko et al, 2020b).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

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Shoot and root thermomorphogenesis are linked by a developmental trade-off

Gaillochet

Burko

Platre

et al. 2020

Preprint

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“…Unlike ARF activators, few reports have demonstrated that ARF repressors are able to interact with other ARF proteins or Aux/IAA proteins (Vernoux et al , 2011). A recent top-notch work revealed a newly identified mechanism where the IAA32 and IAA34 transcriptional repressors are stabilized by the transmembrane kinase 1 (TMK1) at the concave side of the apical hook to regulate ARF gene expression and ultimately inhibit growth (Cao et al , 2019).…”

Section: Introductionmentioning

confidence: 99%

Evolution of Auxin Response Factors in plants characterized by phylogenomic synteny network analyses

Gao

Rui

Oliver

et al. 2019

Preprint

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16Auxin response factors (ARFs) have long been a research focus and represent a class of key regulators of plant growth 17 and development. Previous studies focusing genes from limited number of species were unable to uncover the 18 evolutionary trajectory of this family. Here, more than 3,500 ARFs collected from plant genomes and transcriptomes 19 covering major streptophyte lineages were used to reconstruct the broad-scale family phylogeny, where the early origin 20 and diversification of ARF in charophytes was delineated. Based on the family phylogeny, we proposed a unified six-21 group classification system for angiosperm ARFs. Phylogenomic synteny network analyses revealed the deeply 22 conserved genomic syntenies within each of the six ARF groups and the interlocking syntenic relationships connecting 23 distinct groups. Recurrent duplication events, such as those that occurred in seed plant, angiosperms, core eudicots and 24 grasses contributed the expansion of ARF genes which facilitated functional diversification. Ancestral transposition 25 activities in important plant families, including crucifers, legumes and grasses, were unveiled by synteny network 26 analyses. Ancestral gene duplications along with transpositions have profound evolutionary significance which may 27 have accelerated the functional diversification process of paralogues. Our study provides insights into the evolution of 28 ARFs which will enhance our current understandings for this important transcription factor family. 29 30

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VIK‐Mediated Auxin Signaling Regulates Lateral Root Development in Arabidopsis

Shang,

Wei,

et al. 2024

Advanced Science

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The crucial role of TIR1‐receptor‐mediated gene transcription regulation in auxin signaling has long been established. In recent years, the significant role of protein phosphorylation modifications in auxin signal transduction has gradually emerged. To further elucidate the significant role of protein phosphorylation modifications in auxin signaling, a phosphoproteomic analysis in conjunction with auxin treatment has identified an auxin activated Mitogen‐activated Protein Kinase Kinase Kinase (MAPKKK) VH1‐INTERACTING Kinase (VIK), which plays an important role in auxin‐induced lateral root (LR) development. In the vik mutant, auxin‐induced LR development is significantly attenuated. Further investigations show that VIK interacts separately with the positive regulator of LR development, LATERAL ORGAN BOUNDARIES‐DOMAIN18 (LBD18), and the negative regulator of LR emergence, Ethylene Responsive Factor 13 (ERF13). VIK directly phosphorylates and stabilizes the positive transcription factor LBD18 in LR formation. In the meantime, VIK directly phosphorylates the negative regulator ERF13 at Ser168 and Ser172 sites, causing its degradation and releasing the repression by ERF13 on LR emergence. In summary, VIK‐mediated auxin signaling regulates LR development by enhancing the protein stability of LBD18 and inducing the degradation of ERF13, respectively.

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TMK1-mediated auxin signalling regulates differential growth of the apical hook

Cited by 181 publications

References 40 publications

Shoot and root thermomorphogenesis are linked by a developmental trade-off

Shoot and root thermomorphogenesis are linked by a developmental trade-off

Evolution of Auxin Response Factors in plants characterized by phylogenomic synteny network analyses

VIK‐Mediated Auxin Signaling Regulates Lateral Root Development in Arabidopsis

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