TMEM100 negatively regulated by microRNA-106b facilitates cellular apoptosis by repressing survivin expression in NSCLC

Abstract

Background Non-small-cell lung cancer (NSCLC) is a common malignant tumor. Nevertheless, the 5-year survival rate of NSCLC patients remains poor. Thus, finding critical factors involved in regulating the progression of NSCLC is important for providing potential treatment targets. Methods CCK-8, colony formation, caspase-3 activity, luciferase reporter assay, and western blot assays were utilized in this study. Results We found that TMEM100 was significantly downregulated in NSCLC tissues compared with paired peritumoral tissues. Decreased TMEM100 expression was associated with poor clinical outcomes in NSCLC patients. Moreover, TMEM100 overexpression inhibited colony formation and facilitated apoptosis via repressing survivin expression in NSCLC cells, whereas TMEM100 knockdown had the opposite effect. In addition, miR-106b, a microRNA with controversial roles in different human cancers, was upregulated in NSCLC and directly downregulated TMEM100 expression. The roles of miR-106b in cell survival were mitigated by the restoration of TMEM100. Conclusions The above results indicate that TMEM100 induces cell apoptosis and inhibits cell survival by serving as a tumor suppressor and that miR-106b-degraded TMEM100 expression defines a potentially oncogenic pathway in NSCLC.

GATA binding protein 5-mediated transcriptional activation of transmembrane protein 100 suppresses cell proliferation, migration and epithelial-to-mesenchymal transition in prostate cancer DU145 cells

“…TMEM100 is highly conserved among vertebrates, including mammals, zebrafish and chickens, and it is structurally unique from any known family of proteins [ 9 ]. It has been reported that TMEM100 is associated with cancer tumorigenesis and progression [ 10 ]. Han et al [ 11 ] showed that the expression of TMEM100 was significantly decreased in non-small cell lung cancer (NSCLC) tissues, while TMEM100 overexpression in NSCLC cell lines could inhibit cell proliferation, invasion and migration.…”