TMC-1 attenuates C. elegans development and sexual behaviour in a chemically defined food environment

Zhang, Liusuo; Gualberto, Daisy G.; Guo, Xiaoyan; Correa, Paola; Jee, Chang Hoon; García, L. René

doi:10.1038/ncomms7345

Cited by 39 publications

(53 citation statements)

References 69 publications

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“…The finding that we can detect alkali-activated currents in ADL and ADF soma rather than their sensory endings suggests that TMC-1 is indeed functionally expressed in these neurons but probably not dedicated to detecting alkaline pH in the environment. This is also consistent with the notion that TMC-1 may have other functions (Zhang et al, 2015). Future effort is needed to determine the physiological roles of TMC-1 in these neurons.…”

Section: Resultssupporting

confidence: 91%

“…Two TMC family genes, tmc-1 and tmc-2 , are present in C. elegans , of which tmc-1 has been suggested to encode a Na + -sensitive channel required for salt chemosensation and dietary signaling (Chatzigeorgiou et al, 2013; Zhang et al, 2015). We have characterized tmc-1 gene in the current study, and found that it is required for worms to sense noxious alkaline environment mediated by the ASH nociceptive neurons.…”

Section: Introductionmentioning

confidence: 99%

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TMC-1 Mediates Alkaline Sensation in C. elegans through Nociceptive Neurons

Wang

Liu

et al. 2016

Neuron

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Noxious pH triggers pungent taste and nocifensive behavior. While the mechanisms underlying acidic pH sensation has been extensively characterized, little is known about how animals sense alkaline pH in the environment. TMC genes encode a family of evolutionarily conserved membrane proteins, whose functions are largely unknown. Here, we characterize C. elegans TMC-1 which was suggested to form a Na+-sensitive channel mediating salt chemosensation. Interestingly, we find that TMC-1 is required for worms to avoid noxious alkaline environment. Alkaline pH evokes an inward current in nociceptive neurons, which is primarily mediated by TMC-1 and to a lesser extent by the TRP channel OSM-9. However, unlike OSM-9 which is sensitive to both acidic and alkaline pH, TMC-1 is only required for alkali-activated current, revealing a specificity for alkaline sensation. Ectopic expression of TMC-1 confers alkaline sensitivity to alkali-insensitive cells. Our results identify an unexpected role for TMCs in alkaline sensation and nociception.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

TMC-1 Mediates Alkaline Sensation in C. elegans through Nociceptive Neurons

Wang

Liu

et al. 2016

Neuron

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“…Neuromodulators are involved in metabolic homeostasis and energy regulation in both mammals and Drosophila (Loftus et al 2000;Leibel et al 1995;Obici et al 2002;Harris-Warrick & Marder 1991;Pfaff et al 2008). We asked, therefore, whether manipulation of neuromodulatory circuits might affect respiration in a nutritional status-dependent manner (Zhang et al 2015). Flies were either allowed to feed ad libitum or were starved for 24 h prior to warm-induced Kir2.1 expression.…”

Section: Silencing Aminergic Circuits Affects Co2 Production During Dmentioning

confidence: 99%

Neuromodulatory circuit effects onDrosophilafeeding behaviour and metabolism

Eriksson

Raczkowska

Navawongse

et al. 2016

Preprint

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Animals have evolved to maintain homeostasis in a changing external environment by adapting their internal metabolism and feeding behaviour. Metabolism and behaviour are coordinated by neuromodulation; a number of the implicated neuromodulatory systems are homologous between mammals and the vinegar fly, an important neurogenetic model. We investigated whether silencing fly neuromodulatory networks would elicit coordinated changes in feeding, behavioural activity and metabolism. We employed transgenic lines that allowed us to inhibit broad cellular sets of the dopaminergic, serotonergic, octopaminergic, tyraminergic and neuropeptide F systems. The genetically-manipulated animals were assessed for changes in their overt behavioural responses and metabolism by monitoring eleven parameters: activity; climbing ability; individual feeding; group feeding; food discovery; both fed and starved respiration; fed and starved lipid content; and fed/starved body weight. The results from these 55 experiments indicate that individual neuromodulatory system effects on feeding behaviour, motor activity and metabolism are dissociated.neuromodulation of fly feeding

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“…It is the first multicellular organism to have its genome completely sequenced (C. elegans Sequencing Consortium, 1998), which facilitates bioinformatics analysis. Besides, its genetic tractability, ease of handling and maintenance also make C. elegans become a powerful model system to study development, neurobiology and stress responses (Brenner, 1974;Corsi et al, 2015;Zhang et al 2015b;Hunt, 2016). Only two studies were performed to study pH response in C. elegans.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of the NematodeCaenorhabditis elegansin acidic stress environments

Cong¹,

Yang

Zhang³

et al. 2020

Preprint

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Ocean acidification and acid rain, caused by modern industrial fossil fuels burning, lead to decrease of living environmental pH, which results in a series of negative effects on many organisms. However, the underlying mechanisms of animals'response to acidic pH stress are largely unknown. In this study, we used the nematode Caenorhabditis elegans as an animal model to explore the regulatory mechanisms of organisms'response to pH decline. Two major stress-responsive pathways were found through transcriptome analysis in acidic stress environments. Firstly, when the pH dropped from 6.33 to 4.33, the worms responded to the pH stress by up-regulation of the col, nas and dpy genes, which are required for cuticle synthesis and structure integrity. Secondly, when the pH continued to decrease from 4.33, the metabolism of xenobiotics by cytochrome P450 pathway genes (cyp, gst, ugt, and ABC transporters) played a major role in protecting the nematodes from the toxic substances probably produced by the more acidic environment. At the same time, cuticle synthesis slowed down might due to its insufficient protective ability. Moreover, the systematic regulation pattern we found in nematodes, might also be applied to other invertebrate and vertebrate animals to survive in the changing pH environments. Thus, our data might lay the foundation to identify the master gene(s) responding and adaptation to acidic pH stress in further studies, and might also provide new solutions to improve assessment and monitoring of ecological restoration outcomes, or generate novel genotypes via genome editing for restoring in challenging environments especially in the context of acidic stress through global climate change.

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TMC-1 attenuates C. elegans development and sexual behaviour in a chemically defined food environment

Cited by 39 publications

References 69 publications

TMC-1 Mediates Alkaline Sensation in C. elegans through Nociceptive Neurons

TMC-1 Mediates Alkaline Sensation in C. elegans through Nociceptive Neurons

Neuromodulatory circuit effects onDrosophilafeeding behaviour and metabolism

Transcriptome analysis of the NematodeCaenorhabditis elegansin acidic stress environments

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