TLR4 /NF-ĸB and JAK2/STAT3 signaling pathways: Cellular signaling pathways targeted by cell-conditioned medium therapy in protection against ischemic stroke

Nazarinia, Donya; Dolatshahi, Mojtaba; Faezi, Masoumeh; Maleki, Solmaz Nasseri; Aboutaleb, Nahid

doi:10.1016/j.jchemneu.2021.101938

Cited by 15 publications

(2 citation statements)

References 41 publications

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“…According to a recent investigation, serum CASP3 levels were found to be elevated in cases of intracerebral hemorrhage, and this elevation was found to be associated with both clinical prognosis and severity 42 . The involvement of the JAK2/STAT3 pathway is crucial in the pathological process underlying ischemic stroke 43 , 44 . The increased expression of JAK2/STAT3 observed in individuals suffering from acute ischemic stroke may contribute to cellular inflammation 45 .…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis and experimental validation revealed that Paeoniflorigenone effectively mitigates cerebral ischemic stroke by suppressing oxidative stress and inflammation

Wu,

Tang

2024

Sci Rep

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Inflammation and oxidative stress are becoming more recognized as risk factors for ischemic stroke. Paeoniflorigenone (PA) has diverse pharmacological effects that include anti-inflammatory and antioxidant properties. However, the specific mechanisms by which PA affects cerebral ischemic stroke have not been studied. Our objective was to investigate the potential targets and mechanisms of PA in preventing cerebral ischemic stroke. We obtained the potential targets of PA from the SwissTargetPrediction, Super-PRED, and SEA Search Server databases. The GSE97537 dataset was utilized to identify gene targets related to ischemic stroke. The overlapping targets were imported into the STRING database to construct a protein–protein interaction network, and enrichment analyses were conducted using R software. Rats were pretreated with PA for three weeks before undergoing MCAO and reperfusion. H&E staining, ELISA, and qRT-PCR analyses were then performed to explore the potential mechanisms of PA. In the study, we identified 439 potential targets for PA and 1206 potential targets for ischemic stroke. Out of these, there were 71 common targets, which were found to be primarily associated with pathways related to oxidative stress and inflammation. The results from animal experiments showed that PA was able to improve nerve function and reduce inflammatory cytokines and oxidative stress in the MCAO-induced ischemic stroke model. Additionally, the expression of core genes in the MCAO + HPA group was significantly lower compared to the MCAO group. Our study revealed that the potential mechanisms by which PA prevents ischemic stroke involve oxidative stress and inflammation. These findings provide important theoretical guidance for the clinical use of PA in preventing and managing ischemic stroke.

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Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis and experimental validation revealed that Paeoniflorigenone effectively mitigates cerebral ischemic stroke by suppressing oxidative stress and inflammation

Wu,

Tang

2024

Sci Rep

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show abstract

“…In addition, ROS and inflammatory factors in infarct lesions induce the expression of proinflammatory genes and upregulate inflammation-related cellular signaling pathways such as TLR4/NF-κB, MAPK and JAK/STAT (Dong et al 2019;Nazarinia et al 2021;Satriotomo et al 2006;Yi et al 2007).…”

Section: Pathology Of Ischemic Strokementioning

confidence: 99%

Poststroke lipid droplet accumulation in residing microglia and its influence on inflammation

Wei

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The protective effects of gallic acid and SGK1 inhibitor on cardiac damage and genes involved in Ca2+ homeostasis in an isolated heart model of ischemia/reperfusion injury in rat

Souri,

Badavi,

Dianat

et al. 2024

Naunyn-Schmiedeberg's Arch Pharmacol

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TLR4 /NF-ĸB and JAK2/STAT3 signaling pathways: Cellular signaling pathways targeted by cell-conditioned medium therapy in protection against ischemic stroke

Cited by 15 publications

References 41 publications

Bioinformatics analysis and experimental validation revealed that Paeoniflorigenone effectively mitigates cerebral ischemic stroke by suppressing oxidative stress and inflammation

Bioinformatics analysis and experimental validation revealed that Paeoniflorigenone effectively mitigates cerebral ischemic stroke by suppressing oxidative stress and inflammation

Poststroke lipid droplet accumulation in residing microglia and its influence on inflammation

The protective effects of gallic acid and SGK1 inhibitor on cardiac damage and genes involved in Ca2+ homeostasis in an isolated heart model of ischemia/reperfusion injury in rat

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