TLR4 gene expression in pig populations and its association with resistance to Escherichia coli F18

Wang, J; Pan, Zhangyuan; Zheng, Xianrui; Wu, Zhihua; Su, Xianmin; Zhu, Guo-Qian; Huang, Xiao-Guo; Wu, Shenglong; Bao, Wenbin

doi:10.4238/2013.july.30.1

Cited by 4 publications

(3 citation statements)

References 24 publications

(24 reference statements)

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“…TLR4 is an important member of TLR, which plays an important role in innate immunity and inflammation by sensing pathogen-associated molecular patterns [ 53 ]. It has been shown that TLR4 signaling in macrophages can activate hundreds of genes that contribute to the protection against bacterial infection [ 54 ]. Furthermore, 2‘-5’ oligoadenylates synthesis (OAS) is an antiviral protein induced by interferon, of which OASL belongs to this class of proteins [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

Dietary Supplementation with Mono-Lactate Glyceride Enhances Intestinal Function of Weaned Piglets

Zhang

Xie

et al. 2023

Animals

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Mono-lactate glyceride (LG) is a short-chain fatty acid ester. It has been shown that short-chain fatty acid esters play an important role in maintaining intestinal structure and function. The aim of this study is to investigate the effects of mono-lactate glyceride on growth performance and intestinal morphology and function in weaned piglets. Sixteen 21-day-old weaned piglets of similar weight were distributed arbitrarily to two treatments: The control group (basal diet) and the LG group (basal diet + 0.6% mono-lactate glyceride). The experiment lasted for 21 days. On day 21 of the trial, piglets were weighed, and blood and intestinal samples were collected for further analysis. Results showed that dietary supplementation with 0.6% mono-lactate glyceride decreased (p < 0.05) the diarrhea rate and the contents of malondialdehyde and hydrogen peroxide in the ileum and jejunum and increased (p < 0.05) the expression of intestinal tight junction protein (Occludin) and the activities of superoxide dismutase and catalase in the ileum and colon. In addition, mono-lactate glyceride supplementation could enhance intestinal mucosal growth by increasing (p < 0.05) the mRNA levels of extracellular regulated protein kinases, promote intestinal mucosal water and nutrient transport and lipid metabolism by increasing (p < 0.05) the mRNA levels of b0,+ amino acid transporter, aquaporin 3, aquaporin 10, gap junction protein alpha 1, intestinal fatty acid-binding protein, and lipoprotein lipase, enhance antiviral and immune function by increasing (p < 0.05) the mRNA levels of nuclear factor kappa-B, interferon-β, mucovirus resistance protein II, 2’-5’-oligoadenylate synthetase-like, interferon-γ, C-C motif chemokine ligand 2, and toll-like receptor 4, and enhance antioxidant capacity by increasing (p < 0.05) the mRNA levels of NF-E2-related factor 2 and glutathione S-transferase omega 2 and decreasing (p < 0.05) the mRNA level of NADPH oxidase 2. These results suggested that dietary supplementation with mono-lactate glyceride could decrease the diarrhea rate by improving intestinal antioxidant capacity, intestinal mucosal barrier, intestinal immune defense function, and intestinal mucosal water and nutrient transport. Collectively, dietary supplementation with 0.6% mono-lactate glyceride improved the intestinal function of weaned piglets.

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Section: Discussionmentioning

confidence: 99%

Dietary Supplementation with Mono-Lactate Glyceride Enhances Intestinal Function of Weaned Piglets

Zhang

Xie

et al. 2023

Animals

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“…In the porcine intestinal epithelial IPEC-J2 cell line, the expression and the regulation of TLR4 after infection with an enteric virus has been reported (32). In 35-day-old weaned piglets, a very low level of expression in the jejunal tissue has been reported in comparison to the lung and the spleen (33). We determined the occurrence of CD14 on cells isolated from the intestinal epithelium of suckling and weaned piglets and found only CD45 + immune cells expressing mCD14.…”

Section: Discussionmentioning

confidence: 99%

Probiotic Treatment Decreases the Number of CD14-Expressing Cells in Porcine Milk Which Correlates with Several Intestinal Immune Parameters in the Piglets

et al. 2015

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Modulating the mucosal immune system of neonates by probiotic treatment of their mothers is a promising approach which can only be investigated through the use of animal models. Here, we used sows and their piglets to investigate the impact of a bacterial treatment on the sow’s milk and on the neonate piglet intestinal immune system. In previous experiments, feed supplementation of sows with the probiotic Enterococcus faecium NCIMB 10415 during pregnancy and lactation had been shown to affect intestinal microbiota and cytokine expression of the offspring during the suckling and weaning periods. We therefore investigated the composition of the milk from treated sows in comparison to samples from a control group. In treated sows, the amount of lactose increased, and the somatic cell numbers were reduced. In all milk samples, the percentage of cells expressing membranous CD14 (mCD14) was greater than the fractions of immune cells, indicating expression of mCD14 on mammary epithelial cells. However, in the milk of E. faecium-treated sows, mCD14+ cells were reduced. Furthermore, the number of CD14+ milk cells was positively correlated with the percentages of B cells and activated T cells in the ileal MLN of the piglets. This study provides evidence for the expression of mCD14 by the porcine mammary epithelium, and suggests an immunological effect of mCD14+ milk cells on the piglets’ intestinal immune system. Our study further suggests that mCD14+ mammary epithelial cell populations can be modulated by probiotic feed supplementation of the sow.

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“…85 Expression of TLR4 on macrophages and dendritic cells is increased upon exposure to E. coli, and subsequently, NF-κB activation and TNF-α secretion are both increased. 138 Higher expression of TLR4 does not always result in more effective resistance against E. coli, 139 but some expression seems to be essential for a reaction to E. coli. For example, a pair of twin infants that were both born with a TLR4 deficiency both quickly contracted a very rare brain abscess caused by E. coli.…”

Section: Tlr Rolementioning

confidence: 99%

Arterial Catheterization and Infection: Toll‐like Receptors in Defense against Microorganisms and Therapeutic Implications

Hambsch

Kerfeld

Kirkpatrick

et al. 2015

Clinical Translational Sci

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Radial artery catheterization has become a preferred route over femoral artery catheterization, in order to monitor the blood pressure of hemodynamically unstable patients or for repeated sampling of arterial blood gases. While the incidence of catheter-related infection is lower in the radial artery than the femoral artery, infection remains a major issue that requires attention. In this review of the literature, we discuss infectious complications of radial artery catheterization, with a focus on various risk factors and establishing the most common causative agents. We also critically review the role of the innate immune system involving Toll-like receptors (TLRs) in host-defense, with the goal of establishing a common pathway used by the innate immune system via TLRs to combat the pathogens that most commonly cause infection in radial artery catheterization. If this pathway can be therapeutically manipulated to preemptively attack pathogenic agents, immunomodulation may be an option in reducing the incidence of infection in this procedure.

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TLR4 gene expression in pig populations and its association with resistance to Escherichia coli F18

Cited by 4 publications

References 24 publications

Dietary Supplementation with Mono-Lactate Glyceride Enhances Intestinal Function of Weaned Piglets

Dietary Supplementation with Mono-Lactate Glyceride Enhances Intestinal Function of Weaned Piglets

Probiotic Treatment Decreases the Number of CD14-Expressing Cells in Porcine Milk Which Correlates with Several Intestinal Immune Parameters in the Piglets

Arterial Catheterization and Infection: Toll‐like Receptors in Defense against Microorganisms and Therapeutic Implications

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