TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis

Álvarez-Estrada, Álvaro; Rodríguez-Ferri, Elías Fernando; Martínez-Martínez, Sonia; Álvarez, Belén; Fernández-Caballero, Teresa; Domı́nguez, Javier; Gutiérrez-Martín, César B.

doi:10.1016/j.cimid.2019.02.001

Cited by 7 publications

(3 citation statements)

References 29 publications

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“…Sepsis is attenuated by inhibition of ABCC4 in rats (Xia et al, 2019). Reduction of CD63 increases the mortality rate of sepsis in mice through its function in the immune response ( Yang et al, 2016;Alvarez-Estrada et al, 2019). The above evidence strongly supports our view that m 6 A methylation plays an extremely important role in the pathological basis of sepsis.…”

Section: Discussionsupporting

confidence: 72%

Integration Analysis of m6A-SNPs and eQTLs Associated With Sepsis Reveals Platelet Degranulation and Staphylococcus aureus Infection are Mediated by m6A mRNA Methylation

et al. 2020

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Sepsis is a major threat with high mortality rate for critically ill patients. Response to pathogen infection by the host immune system is a key biological process involved in the onset and development of sepsis. Heterogeneous host genome variation, especially single nucleotide polymorphisms (SNPs), has long been suggested to contribute to differences in disease progression. However, the function of SNPs located in noncoding regions remains to be elucidated. Recently, m 6 A mRNA modification levels were revealed to differ at SNPs. As m 6 A is a crucial regulator of gene expression, these SNPs might control genes by changing the m 6 A level on mRNA. To investigate the potential role of m 6 A SNPs in sepsis, we integrated m 6 A-SNP and expression quantitative trait loci (eQTLs) data. Analysis revealed 15,720 m 6 A-cis-eQTLs and 381 m 6 A-trans-eQTLs associated with sepsis. We identified 1321 genes as locations of m 6 A-cis-eQTLs. These were enriched in platelet degranulation and Staphylococcus aureus infection pathways, which are vital for the pathophysiological process of sepsis. We conclude that m 6 A modification of mRNA plays a very important role in sepsis, with m 6 A-cis-eQTLs potentially having the most effect on individual variation in sepsis progression.

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Section: Discussionsupporting

confidence: 72%

Integration Analysis of m6A-SNPs and eQTLs Associated With Sepsis Reveals Platelet Degranulation and Staphylococcus aureus Infection are Mediated by m6A mRNA Methylation

et al. 2020

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“…The expression of five immune-related genes was unfavorably associated with all three qNUR traits (Table 4 ): tec protein tyrosine kinase ( TEC ), TLR2 , adhesion G protein-coupled receptor E1 ( ADGRE1 ), CD163 , and FAM111A (Table 4 ). TLR2 recognizes many bacterial, fungal, viral, and certain endogenous substances, and is involved in activation of innate immunity [ 26 , 27 ]; ADGRE1 is the marker gene for myeloid differentiation in pigs [ 28 ]; expression of CD163 is increased during infection with A. pleuropneumoniae [ 29 ] and H. parasuis [ 30 ]; FAM111A plays a role in inhibiting viral genome replication [ 31 ]. In addition, GSEA showed that genes for which an increase in expression was unfavorably associated with qNUR traits were enriched for GO terms related to immunity (FDR < 0.001; Fig.…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of the blood transcriptome of young healthy pigs and its relationship with subsequent disease resilience

et al. 2021

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Background Disease resilience, which is the ability of an animal to maintain performance under disease, is important for pigs in commercial herds, where they are exposed to various pathogens. Our objective was to investigate population-level gene expression profiles in the blood of 912 healthy F1 barrows at ~ 27 days of age for associations with performance and health before and after their exposure to a natural polymicrobial disease challenge at ~ 43 days of age. Results Most significant (q < 0.20) associations of the level of expression of individual genes in blood of young healthy pigs were identified for concurrent growth rate and subjective health scores prior to the challenge, and for mortality, a combined mortality-treatment trait, and feed conversion rate after the challenge. Gene set enrichment analyses revealed three groups of gene ontology biological process terms that were related to disease resilience: 1) immune and stress response-related terms were enriched among genes whose increased expression was unfavorably associated with both pre- and post-challenge traits, 2) heme-related terms were enriched among genes that had favorable associations with both pre- and post-challenge traits, and 3) terms related to protein localization and viral gene expression were enriched among genes that were associated with reduced performance and health traits after but not before the challenge. Conclusions Gene expression profiles in blood from young healthy piglets provide insight into their performance when exposed to disease and other stressors. The expression of genes involved in stress response, heme metabolism, and baseline expression of host genes related to virus propagation were found to be associated with host response to disease.

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“…In H. parasuis, Siglecs (Sialic acid-binding Ig-like lectins) is another receptor constituting the inhibitory mechanism against an exacerbated innate response initiated by PRRs that could be dangerous to the host [40]. Additionally, CD163 (M130/p155) has also been reported to be a cell surface receptor of peripheral blood mononuclear cells (PBMCs) from pigs, and it plays crucial roles in immune response and bacterial sepsis caused by H. parasuis [41].…”

Section: Introductionmentioning

confidence: 99%

Polyamine Transport Protein PotD Protects Mice against Haemophilus parasuis and Elevates the Secretion of Pro-Inflammatory Cytokines of Macrophage via JNK–MAPK and NF–κB Signal Pathways through TLR4

Dai

Yang

et al. 2019

Vaccines

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The potD gene, belonging to the well-conserved ABC (ATP-binding cassette) transport system potABCD, encodes the bacterial substrate-binding subunit of the polyamine transport system. In this study, we found PotD in Haemophilus (Glaesserella) parasuis could actively stimulate both humoral immune and cellular immune responses and elevate lymphocyte proliferation, thus eliciting a Th1-type immune response in a murine immunity and infection model. Stimulation of Raw 264.7 macrophages with PotD validated that Toll-like receptor 4, rather than 2, participated in the positive transcription and expression of pro-inflammatory cytokines IL–1β, IL–6, and TNF–α using qPCR and ELISA. Blocking signal-regulated JNK–MAPK and RelA(p65) pathways significantly decreased PotD-induced pro-inflammatory cytokine production. Overall, we conclude that vaccination of PotD could induce both humoral and cellular immune responses and provide immunoprotection against H. parasuis challenge. The data also suggest that Glaesserella PotD is a novel pro-inflammatory mediator and induces TLR4-dependent pro-inflammatory activity in Raw 264.7 macrophages through JNK–MAPK and RelA(p65) pathways.

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TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis

Cited by 7 publications

References 29 publications

Integration Analysis of m6A-SNPs and eQTLs Associated With Sepsis Reveals Platelet Degranulation and Staphylococcus aureus Infection are Mediated by m6A mRNA Methylation

Integration Analysis of m6A-SNPs and eQTLs Associated With Sepsis Reveals Platelet Degranulation and Staphylococcus aureus Infection are Mediated by m6A mRNA Methylation

Quantitative analysis of the blood transcriptome of young healthy pigs and its relationship with subsequent disease resilience

Polyamine Transport Protein PotD Protects Mice against Haemophilus parasuis and Elevates the Secretion of Pro-Inflammatory Cytokines of Macrophage via JNK–MAPK and NF–κB Signal Pathways through TLR4

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