TLC detection of chemical interactions of vitamins A and D with drugs

Ramić, Alan; Medić-Šarič, Marica; Turina, S.; Jasprica, Ivona

doi:10.1556/jpc.19.2006.1.5

Cited by 8 publications

(6 citation statements)

References 29 publications

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“…Vitamin A deficiency is rarely found in Western countries; although biochemical interactions between vitamin A and different drugs have often been described [40], only patients with specific gastrointestinal diseases are at risk [5]. Vitamin E has been promoted as a strategy to prevent atheroscleosis, cardiovascular disease and cancer due to its properties such as inhibition of oxidation and inflammation, increasing release of prostacyclin, promoting blood vessel dilatation and reducing platelet aggregation [5,41].…”

Section: Discussionmentioning

confidence: 99%

“…Although the intake of vitamin D was comparable in both groups, the percentage of inadequately supplied subjects increased with the amount of drugs taken per day and was significantly higher (98%) in those elderly taking ≥3 drugs per day than in those who took a maximum of 2 medications daily (82%). Interactions between vitamin D and different drugs have often been described [40], and further data suggest that several therapeutic agents can place subjects at risk for significant bone loss, osteoporosis and fracture [46]. In addition to drug-related adverse effects, vitamin D status could also be affected negatively by other age-related factors, e.g.…”

Section: Discussionmentioning

confidence: 99%

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Intake of Medication and Vitamin Status in the Elderly

Fabian

Bogner²,

Kickinger³

et al. 2011

Ann Nutr Metab

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Background/Aims: An inadequate vitamin status is associated with higher morbidity and frailty in the elderly and might be due to medication. This study aimed to evaluate the status of several vitamins in relation to regular intake of medication in this population. Methods: A total of102 non-institutionalized subjects aged 70–90 years were recruited. Plasma levels of vitamins A, D, E, K and C were determined by HPLC. The functional parameters of vitamins B₁, B₂ and B₆, i.e. the activities of the erythrocyte enzymes transketolase, glutathione reductase and glutamic oxaloacetic transaminase were analyzed photometrically; plasma folate and vitamin B₁₂ were determined by RIA. Results: The status of vitamins A, E and C was generally satisfactory. Eighty-eight percent and 42% of participants were deficient in vitamins D and K, respectively, as were 29% in B₆; up to 10% were deficient in vitamins B₁, B₂, B₁₂ and folate. A considerable percentage of participants was, however, at risk for vitamin deficiencies (vitamins B₁, B₆, B₁₂ and folate: 20–30%; vitamin B₂: 60%). Regular intake of maximally 2 drugs per day was not adversely related to the status of several vitamins; intake of ≧3 drugs per day was significantly negatively associated with the status of vitamins D, K, B₆ and folate. Conclusion: Daily intake of ≧3 drugs was found to be adversely associated with the status of some vitamins in the elderly. Hence, the medication schedule and nutritional status of these subjects should be monitored closely to ensure that the daily micronutrient requirement is fulfilled.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Intake of Medication and Vitamin Status in the Elderly

Fabian

Bogner²,

Kickinger³

et al. 2011

Ann Nutr Metab

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“…The interactions were most common with acetylsalicylic acid. At the moment this has no practical value as further in vivo studies have to demonstrate applicability of this model [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Based on the procedure developed by Ramić et al . [ 21 ] chemical interactions were studied using the optimized chromatographic system for polyphenols. Interactions of 19 polyphenols and over-the-counter (OTC) analgesic (acetylsalicylic acid, paracetamol, diclofenac, ibuprofen and ketoprofen) and vitamins (C and E) were examined.…”

Section: Reviewmentioning

confidence: 99%

From functional food to medicinal product: Systematic approach in analysis of polyphenolics from propolis and wine

Medić-Šarič

Rastija

Bojić

et al. 2009

Nutr J

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In the last decade we have been working on standardization of propolis extract and determination of active constituents of wine those are rich in polyphenolics and have nutritional as well as therapeutic value. Here we are summarizing our results and providing overview on systematic approach how to analyse natural products rich in flavonoids and phenolic acids.Chromatographic methods (thin layer chromatography and high performance liquid chromatography) were used for identification, quantification, and characterization of individual flavonoid or phenolic acid. Total content of active constituents and antioxidant activity were determined by spectrophotometry. Pharmacokinetic parameters were determined by high performance liquid chromatography and using appropriate software. Quantitative structure-activity relationship study of antioxidant activity was conducted, as well as assessment of prolonged propolis supplementation on antioxidative status of organism.Thin layer chromatography-densitometry has been proven as quick and reliable method for standard analysis of propolis and wine; the best mobile phase being chloroform -methanol -formic acid (98-100%) in ratio 44 : 3.5 : 2.5 (v/v). Higher number of polyphenolics was determined by high performance liquid chromatography; 15 compared to 9 by thin layer chromatography. Interactions in situ with acetylsalicylic acid were detected with most of polyphenolics analysed. Plasma protein binding and blood-barrier penetration was greatest for flavone. The interactions with human serum albumin have been grater than 95% for all flavonoids analysed. The prolonged propolis consumption increased superoxide dismutase activity.The necessity of standardization of natural products and their registration as functional nutraceuticals demand easy, quick and inexpensive methods of analysis. In this work we provided overview of analytical part for polyphenolics that could be used as data for possible registration of final products either as functional food or medicinal product. This feature introduces the readers to the authors' research through a concise overview of the selected topic. Reference to important work from others in the field is included.

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“…According to Ramić et al, 7 when one component of the developing solvent mixture comes from the vapour phase onto the plate, which is the case in this work, it changes the characteristics of the mobile phase; it causes the change of distribution coefficient α=σ S /σ M . The concentration of the developing solvent sorbed from the vapour phase into the mobile phase is proportional to the partial pressure, and because the RF value is a function of α, we must control the partial pressure of the solvent.…”

Section: Discussionmentioning

confidence: 86%

A Novel Method to Chromatographically Resolution Of Sulphonamides By Vapour-Programmed Thin-Layer Chromatography

Choukaife

Aljerf²

2017

MOJBOC

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All developing solvent components were reagent grade (Merck). Liquid compositions are given by volume. Solutions of sulphonamides (0.1% w/v) in acetone were used as standards. A coloured substance, 4-nitroaniline, was used as a reference compound. TLC apparatus and proceduresSilica Gel GF 254 (Merck) was used as adsorbent -30g/60ml of demineralised water to prepare 5 plates, 20x20cm, for a layer thickness of 0.25mm. After spreading (Desaga apparatus) the plates were air dried for 15min, heated for 30min at 110°C in an oven with a fan, then cooled and stored in a desiccator. Samples, 3µg, were applied to the plates and dried with the aid of a cold air blower, 2.5cm from the lower edge of the plate and 1cm apart. For VP-TLC strips of the gel, 0.5cm wide at the sides and the lower edge, were removed from the plates. After spotting, the plates were left in the ambient atmosphere for at least 15min to ensure water vapour adsorption in equilibrium with the ambient relative humidity. A VP-chamber (manufactured by Desaga) (Scheme 1) was used for development, while normal tank chambers (N-chambers, Shandon) were used as controls. N-chambers are cuboids twin-trough chambers. The trough chamber was made of rolled brass and the troughs were directly milled out. The top surface of the trough chamber was also milled to obtain a completely flat surface. After milling, the trough chamber and the other brass parts are Ni-and Cr-plated. Brass was chosen to ensure sufficient thermo conductivity. A space of 1mm was needed between the trough chamber and the solvent reservoir to prevent disappearance of solvent by capillary action between these parts. Troughs in the VP-chamber (Scheme 1) were each filled with about 5ml of the appropriate liquid mixture, and then the plate was fixed in position. After a 10min equilibrium period, the solvent reservoir (40cmx1cmx2cm) was filled with 25ml of developing solvent. N-chambers contained 100ml of developing solvent and were saturated with solvent vapour by lining the walls with filter paper. After 45-60min the plate was introduced and development started. The developing solvent was allowed to run 16cm beyond the starting point, whereas in VP-TLC continuous development was used for the time given in minutes. All experiments were carried out at 21±1°C at a relative humidity of 27-41%. Within this range the separations were reproducible. Detection and photographyDetection was carried out in UV light of 254nm (Camag, Universal lamp), followed by photography under two such lamps on Agfacolor CT 18 diapositive film with an Asahi Pentax type SV camera with 49mm UV ghostless filter. Exposure was 3sec, distance 70cm and aperture 5.6.

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TLC detection of chemical interactions of vitamins A and D with drugs

Cited by 8 publications

References 29 publications

Intake of Medication and Vitamin Status in the Elderly

Intake of Medication and Vitamin Status in the Elderly

From functional food to medicinal product: Systematic approach in analysis of polyphenolics from propolis and wine

A Novel Method to Chromatographically Resolution Of Sulphonamides By Vapour-Programmed Thin-Layer Chromatography

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