TLC densitometric method for the preliminary evaluation of fucoxanthin-based products

Abstract

Studies have evidenced the biological properties of fucoxanthin and the commercialisation of various nutritional supplements based on seaweed extracts that claim high fucoxanthin content is constantly growing. However, it was demonstrated that fucoxanthin is highly susceptible to degradation, and in this context the need for simple and reliable analytical methods for fucoxanthin analysis is evident. This article presents a simple thin-layer chromatography (TLC) densitometric method for the determination of fucoxanthin from Undaria pinnatifida extracts and commercial formulations. Fucoxanthin was separated on TLC silica gel 60 F254 plates with acetone:n-hexane 6:4 v/v as mobile phase. Densitometric analysis was performed using the Image J image-processing program available online. Calibration data revealed good linear relationship (R(2) = 0.9955) between peak area and concentration of fucoxanthin. The repeatability was 2.0 (% CV), and intra-day and inter-day precisions were 1.5 and 2.0 (% RSD), respectively. The proposed method provides an inexpensive tool in preliminary evaluation of fucoxanthin-based products.

Rapid and feasible TLC screening of tea tree oil commercial samples

“…Currently, the most widely used technique for the analysis of EOs is GC-MS. Alike GC-MS, TLC plays an important role in the qualitative and quantitative analysis of compounds present in multicomponent mixtures and even if it is not considered the technique of choice, TLC has in many cases some advantages over other chromatographic techniques [24][25][26] . Indeed, our results show that TLC is an immediate, simplest and feasible analytical tool to check the presence and to identify known compounds and/or adulterants in TTOs.…”

Optimasi Metode dan Penentuan Batas Deteksi untuk Analisis Kadar Fukosantin dalam Sargassum ilicifolium (Turner) C.Agardh Menggunakan KLT-Densitometri

“…Penetapan kadar fukosantin dapat dilakukan dengan berbagai metode seperti Kromatografi Cair Kinerja Tinggi (KCKT) (Crupi et al, 2013;Wrightl, 1987) , HPTLC (Wrightl, 1987),kromatografi Lapis Tipis (KLT)-Densitometri (Piovan et al, 2014) dan Spektrofotometri UV-Vis (Sulistiyani et al, 2021). Kromatografi Lapis tipis (KLT) densitometer merupakan instrumen yang umum digunakan dalam penetapan kadar marker dalam bahan alam.…”