TLC-densitometric analysis of clotrimazole and metronidazole in combined dosage forms

Meshram, Dhananjay; Bagade, Shashikant B.; Tajne, Madhukar R.

doi:10.1556/jpc.21.2008.4.10

Cited by 7 publications

(5 citation statements)

References 15 publications

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“…The antifungal agent clotrimazole and antibiotic metronidazole were determined in tablet and cream formulations with the toluene−EtAc−MeOH−HAc (55:20:6:1) mobile phase. Respective linear response ranges were 0.4−0.8 and 2−4 μg/band; RSD values were <1% for intraday, interday, and robustness precision studies; and recoveries were 99.5−99.2% .…”

Section: Quantitative Analysismentioning

confidence: 99%

Planar Chromatography

Sherma

2010

Anal. Chem.

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Chemical Abstracts (SciFinder Scholar) cited 2583 references containing the search phrase "thin layer chromatography" during this review period, compared to 2290 citations for the last review period, November 1, 2005 to November 1, 2007; the actual totals are higher than these numbers because all worldwide journals publishing papers on TLC are not surveyed by this service, and all papers abstracted by it may not be recovered using only this L-tyrosine; and lipophilicity determination of some angiotensin converting enzyme (ACE) inhibitors.Papers in another special issue on TLC of the same journal [2009, 32 (9)], also guest edited by Sherma and Fried, had papers on TLC-bioautogram analysis of vitamin B 12 compounds from Japanese anchovy products; determination of the fatty acid

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Section: Quantitative Analysismentioning

confidence: 99%

Planar Chromatography

Sherma

2010

Anal. Chem.

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“…Scientific literature describes methods using thin-layer chromatography for the determination of selected 5-nitoimidazole in the presence of another drug, e.g. metronidazole and diiodohydroxychinoline [16], diiodohydroxyquine [17], spiramycin [18], furazolidone [19,20], 2 loperamide [19], tetracycline hydrochloride [21], ciprofloxacin [22], diloxamide furoate [23], clotrimazole [24] as well as tinidazole and clotrimazole [25,26], omeprazole [27], clarithromycin [27], fluconazole [28,29], norfloxacin [30][31][32], ciprofloxacin [33].…”

Section: Introductionmentioning

confidence: 99%

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

Pyka-Pająk

2024

Preprint

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An easy and short-time TLC-densitometric method was developed for the separation of metronidazole, secnidazole, ornidazole, tinidazole, and 2-methyl-5-nitroimidazole and for the determination of metronidazole and tinidazole in Metronidazole Polpharma and Tinidazolum Polpharma tablets. Analyzes were performed on chromatographic plates precoated with silica gel 60F254 using the optimal mobile phase: chloroform + methanol + diethylamine in a volume ratio of 9:1:1. The method has been validated. The intraday and interday precision values for the three different concentrations ranged from 0.99% to 1.48% and 0.89% to 1.76%, and the precision values ranged from 1.13% to 2.48% and 0.95% to 2.49% for metronidazole and tinidazole, respectively. The limit of quantification (LOQ) was 0.036 and 0.066 µg/spot for metronidazole and tinidazole, respectively. The mean recovery was 103.1% and 100.6% for metronidazole and tinidazole, respectively. The content of metronidazole and tinidazole in tablets in relation to the content declared by the manufacturer was 101.3% and 99.8%, respectively. The obtained results were verified using the pharmacopoeial method. The presented method is fast, sensitive, precise, selective, accurate and robust. It allows for the analysis of several samples on one chromatography plate at the same time.

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“…Metronidazole (M), secnidazole (S), ornidazole (O), and tinidazole (T) are drugs commonly used to treat protozoan infestations and infections caused by anaerobic bacteria. The scientific literature describes using thin-layer chromatography methods for the determination of selected 5-nitoimidazole in the presence of another drug, e.g., metronidazole and di-iodohydroxychinoline [16], diiodohydroxyquine [17], spiramycin [18], furazolidone [19,20], loperamide [19], tetracycline Processes 2024, 12, 643 2 of 17 hydrochloride [21], ciprofloxacin [22], diloxamide furoate [23], and clotrimazole [24] as well as tinidazole and clotrimazole [25,26], omeprazole [27], clarithromycin [27], fluconazole [28,29], norfloxacin [30][31][32], and ciprofloxacin [33]. TLC methods presented in the scientific literature are often not fully validated.…”

Section: Introductionmentioning

confidence: 99%

Sensitive and Cost-Effective TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Tablets

Pyka-Pająk

2024

Processes

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A sensitive, easy-to-use, fast, and cost-effective TLC-densitometric method was developed for the separation of metronidazole, secnidazole, ornidazole, tinidazole, and 2-methyl-5-nitroimidazole and for the determination of metronidazole and tinidazole in Metronidazole Polpharma and Tinidazolum Polpharma tablets. Analyses were performed on chromatographic plates precoated with silica gel 60F254 using chloroform + methanol + diethylamine in a volume ratio of 9:1:1 as the optimal mobile phase. The method has been validated. The intraday and interday precision values for the three different concentrations ranged from 0.99% to 1.48% and 0.89% to 1.76%, and the precision values ranged from 1.13% to 2.48% and 0.95% to 2.49% for metronidazole and tinidazole, respectively. The limit of quantification (LOQ) was 0.036 and 0.066 µg/spot for metronidazole and tinidazole, respectively. The mean recovery was 103.1% and 100.6% for metronidazole and tinidazole, respectively. The content of metronidazole and tinidazole in tablets in relation to the content declared by the manufacturer was 101.3% and 99.8%, respectively. The obtained results were verified using the pharmacopeial method. The presented method is fast, sensitive, precise, selective, accurate, and robust. It allows for the analysis of several samples on one chromatography plate at the same time.

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TLC-densitometric analysis of clotrimazole and metronidazole in combined dosage forms

Cited by 7 publications

References 15 publications

Planar Chromatography

Planar Chromatography

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

Sensitive and Cost-Effective TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Tablets

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