TLC-densitometric analysis of allantoin in Symphytum officinale L. roots

Abstract

A TLC-densitometric method for determination of allantoin in Symphytum officinale root was developed. Densitometric quantification of allantoin was carried out on TLC Si60 plates with butanol-50 % methanol/formic acid, 66.5:33.2:0.3 (V/V/V) as developing solvent, at a wavelength of 190 nm. The method was preliminarily validated in terms of specificity, linearity, precision, limit of detection, limit of quantification, recovery and robustness. The results of TLC quantification were compared with HPLC analysis carried out on a HILIC Luna NH 2 100A column, with mobile phase consisting of acetonitrile/water 80:20 (V/V) and UV detection at 190 and 210 nm. Allantoin content was determined in two herbal products and it varied from 0.94 to 2.09 %, depending on the producer, and was in agreement with literature reports.

Allantoin from Valuable Romanian Animal and Plant Sources with Promising Anti-Inflammatory Activity as a Nutricosmetic Ingredient

“…The best results were identified for UME plant extracts and FS1 water soluble snail fraction. These samples showed high antioxidant activities due to the fact that in the ultrasound methanolic extract (UME) from the roots as well as in the water-soluble fraction of snail mucus (FS1) were observed a considerable amount of allantoin but also the presence of other bioactive compounds, these results agreeing with the data from the literature [7,20,24,53]. Figure 7 shows that the antioxidant capacity is lower for A, EUE and FM samples.…”

“…The extraction methods from plants and snails mucus both in our study and in other studies in the literature [5,22,53], led to the extraction of allantoin but also of other bioactive compounds, with a higher content of them in the samples extracted with methanol from S. officinale roots (UME) and the water-soluble fraction of snail mucus (FS1), therefore, this may be the reason why the two samples showed the best antioxidant properties in the iron ion chelating method. The extraction methods from plants and snails mucus both in our study and in other studies in the literature [5,22,53], led to the extraction of allantoin but also of other bioactive compounds, with a higher content of them in the samples extracted with methanol from S. officinale roots (UME) and the water-soluble fraction of snail mucus (FS1), therefore, this may be the reason why the two samples showed the best antioxidant properties in the iron ion chelating method.…”

“…Human albumin denaturation test was carried out for this purpose for the selected allantoin rich samples (Figure 9). The extraction methods from plants and snails mucus both in our study and in other studies in the literature [5,22,53], led to the extraction of allantoin but also of other bioactive compounds, with a higher content of them in the samples extracted with methanol from S. officinale roots (UME) and the water-soluble fraction of snail mucus (FS1), therefore, this may be the reason why the two samples showed the best antioxidant properties in the iron ion chelating method.…”

“…Fu et al reported a concentration of allantoin in snail mucus of 47-52 mg/L (HA) [10]. In Symphytum officinale root, the allantoin content reported by Kimel et al was of 0.94-2.10% in raw material in alcoholic extracts [53]. ainability 2021, 13, x FOR PEER REVIEW Allantoin concentration and distribution in mucus fractions varied grea allantoin content was obtained in FS1 (22.051 ± 0.64 μg/mL) compared to the cessed fraction FS2 (4.102 ± 0.08 μg/mL).…”

Determination of Allantoin Levels as a Result of Optimization of Ionic Liquid-Ultrasound Assisted Extraction of Comfrey Leaves by TLC-Densitometry

“…Therefore the importance of Comfrey in treatment with allantoin compounds. TLC densitometry is often used because analysis is fast, easy, simple, and has a high sensitivity [16], [17].…”