TL-DNA transformation decreases ABA level

Julliard, Jacques; Pelese, Florence; Sotta, Bruno; Maldiney, Régis; Primard-Brisset, Catherine; Jouanin, Lise; Pelletier, Georges; Miginiac, Émile

doi:10.1034/j.1399-3054.1993.880418.x

Cited by 4 publications

(7 citation statements)

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“…In the present study, the highest frequency of roots (mean number of roots per shoots) was achieved in 1/2 MS, but with the increasing concentration of NAA in the medium, there is no improvement in rooting frequency of differentiated shoots from hairy roots-derived P. cablin calluses when NAA was added to the rooting medium. Reports have shown that when the T-DNA fragment or even a single rol gene of Ri plasmid of A. rhizogenes was integrated and expressed into the genome of plant cells, it not only resulted in changes of cell endogenous phytohormone levels (Juiliard et al 1993;Shi et al2000), but also influenced or enhanced the hormone sensitivity in plant cells (Nilsson et al1993). The changes in sensitivity and content of endogenous growth regulators in plant cells undoubtedly affected the response effect of plant cells to exogenous growth regulators.…”

Section: Discussionmentioning

confidence: 98%

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Shi

Yong-Yue

Tie-Shan

et al. 2011

Plant Cell Tiss Organ Cult

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An efficient transformation system for the medicinal and aromatic plant, Pogostemon cablin Benth was developed by using agropine-type Agrobacterium rhizogenes ATCC15834. Hairy roots formed directly from the cut edges of leaf explants or via callus stage 8 days after inoculation with the bacterium. The highest frequency of leaf explant transformation by Agrobacterium rhizogenes ATCC15834 was about 80% after infection for 25 days. Hairy roots grew rapidly on plant growth regulators (PGRs)-free Murashige and Skoog (MS) or 6,7-V medium and had characteristics of transformed roots such as fast growth and high lateral branching. The PCR amplification showed that rol genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. The hairy root line, PL6, grew very slowly in the first 8 days, then grew very quickly between day 8 and day 24. The optimum medium for callus induction of hairy roots consisted of 2.0 mg l -1 benzyladenine (BA) and 0.1 mg/l a-naphthaleneacetic acid (NAA); while optimum medium for adventitious shoot regeneration from these cultures consisted of 0.1 mg l -1 BA and 0.1 mg l -1 NAA. Adventitious shoots could be rooted on 1/2MS. Southern blot analysis confirmed that rol genes of TL-DNA of Ri plasmid was integrated with at least three copies into the genome of hairy rootsregenerated P. cablin plants. The results presented provide a solid foundation for production of patchouli essential oil from hairy roots or its regenerated plants and also provide possibilities for utilization of artifical polyploidization or chemical mutation of hairy roots for improving germplasm and breeding of a new cultivar of P. cablin.

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Section: Discussionmentioning

confidence: 98%

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Shi

Yong-Yue

Tie-Shan

et al. 2011

Plant Cell Tiss Organ Cult

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“…ABA measurement was performed on dry seeds and 26‐day‐old plantlets as described in Julliard et al. (1993).…”

Section: Methodsmentioning

confidence: 99%

“…ABA measurement was performed on dry seeds and 26-day-old plantlets as described in Julliard et al (1993). ABA extractions were performed at 4°C in darkness for 60 h from about 100 mg of tissue powder in 5 ml of 80% aqueous methanol supplemented with butylhydroxytoluene (40 mg l )1 ) as an antioxidant.…”

Section: Aba Measurementmentioning

confidence: 99%

The phs1‐3 mutation in a putative dual‐specificity protein tyrosine phosphatase gene provokes hypersensitive responses to abscisic acid in Arabidopsis thaliana

et al. 2006

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SummaryThe plant hormone abscisic acid (ABA) controls numerous physiological traits: dormancy and germination of seeds, senescence and resistance to abiotic stresses. In order to get more insight into the role of protein tyrosine phosphatase (PTP) in ABA signalling, we obtained eight homozygous T-DNA insertion lines in Arabidopsis thaliana PTP genes. One mutant, named phs1-3, exhibited a strong ABA-induced inhibition of germination as only 26% of its seeds germinated after 3 days instead of 92% for the Columbia (Col-0) line. Genetic and molecular analyses of phs1-3 showed that it bears a unique T-DNA insertion in the promoter of the gene and that the mutation is recessive. PHS1 expression in the mutant is about half that of the Col-0 line. The upregulation of two ABA-induced genes (At5g06760, RAB18) and the downregulation of two ABA-repressed genes (AtCLC-A, ACL) are enhanced in the phs1-3 mutant compared with the wild-type. The 'in planta' aperture of phs1-3 stomata is reduced and the inhibition of the light-induced opening of stomata by ABA is stronger in phs1-3 leaves than in Col-0 leaves. Finally, PHS1 expression is upregulated in the presence of ABA in both phs1-3 and Col-0 but more intensively in the mutant. Thus, phs1-3 is hypersensitive to ABA. Taken together, these results show that PHS1, which encodes a dual-specificity PTP, is a negative regulator of ABA signalling.

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“…In order to simplify the notation, we refer to it as the ABA‐BSA concentration. The chemical stability of the conjugate was controlled by C18‐HPLC fractionation and ELISA ( Julliard et al . 1993 ).…”

Section: Methodsmentioning

confidence: 99%

“…In order to simplify the notation, we refer to it as the ABA-BSA concentration. The chemical stability of the conjugate was controlled by C18-HPLC fractionation and ELISA (Julliard et al, 1993). 3 HABA uptake Ten ml of the cell suspension were equilibrated at pH 5, 6, 7 or 8 as described above, and 10 -5 M ABA with 2 µCi 3 HABA was added.…”

Section: Synthesis Purification and Characterization Of The Aba-bsa mentioning

confidence: 99%

Induction of RAB18 gene expression and activation of K⁺ outward rectifying channels depend on an extracellular perception of ABA in Arabidopsis thaliana suspension cells

et al. 1999

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SummaryImportant progress has been made regarding the characterization of the ABA signalling components using genetic and molecular approaches (Leung and Giraudat, 1998). However, we do not yet know the mechanism of ABA perception. Conflicting results concerning the site of ABA perception have been published. The prevailing view is that since ABA controls many responses, different sites of perception for ABA might exist. In order to establish the cellular localisation of the ABA receptors in Arabidopsis thaliana suspension cells, we developed two physiological tests based upon the capacity of impermeant ABA-BSA conjugate to mimic permeant free ABA effects. We show that purified ABA-BSA conjugate is able to trigger RAB18 gene expression and that this response is strictly due to the natural (⍣)-ABA enantiomer. The rate of RAB18 gene expression was independent of the level of ABA uptake by the cells. Using the voltage-clamp technique we show that ABA-BSA, similarly to ABA, evokes a membrane depolarization and activates time-and voltage-dependent outward rectifying currents (ORC). We demonstrate that these ORC are due to a K ⍣ efflux as assessed by tail currents and specific inhibition by both tetraethylammonium (TEA) and Ba 2⍣ . These observations provide evidence in favour of an extracellular site for ABA perception.

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TL-DNA transformation decreases ABA level

Cited by 4 publications

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Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

The phs1‐3 mutation in a putative dual‐specificity protein tyrosine phosphatase gene provokes hypersensitive responses to abscisic acid in Arabidopsis thaliana

Induction of RAB18 gene expression and activation of K⁺ outward rectifying channels depend on an extracellular perception of ABA in Arabidopsis thaliana suspension cells

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TL-DNA transformation decreases ABA level

Cited by 4 publications

References 0 publications

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon Cablin

The phs1‐3 mutation in a putative dual‐specificity protein tyrosine phosphatase gene provokes hypersensitive responses to abscisic acid in Arabidopsis thaliana

Induction of RAB18 gene expression and activation of K+ outward rectifying channels depend on an extracellular perception of ABA in Arabidopsis thaliana suspension cells

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Induction of RAB18 gene expression and activation of K⁺ outward rectifying channels depend on an extracellular perception of ABA in Arabidopsis thaliana suspension cells