Tityus serrulatus venom peptidomics: Assessing venom peptide diversity

Rates, Breno; Ferraz, Karla K.F.; Borges, Márcia Helena; Richardson, Michael; Lima, Maria Elena de; Pimenta, Adriano M.C.

doi:10.1016/j.toxicon.2008.07.010

Cited by 64 publications

(55 citation statements)

References 49 publications

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“…Butantoxin, on the other hand, is a potassium channel blocker purified from T. serrulatus, T. bahiensis and T. stigmurus (Holaday et al, 2000). Peptides and enzymatic proteins present in the composition of the venom from some scorpion species have been investigated by different approaches such as transcriptomic and proteomic methods (Kalapothakis et al, 2001;Pimenta et al, 2001;Goudet et al, 2002;Schwartz et al, 2007;Ma et al, 2009Ma et al, , 2010Ruiming et al, 2010;Morgenstern et al, 2011;D'Suze et al, 2009;Batista et al, 2006Batista et al, , 2007Bringans et al, 2008;Rates et al, 2008;Ma et al, 2012;Diego-Garcia et al, 2012;Almeida et al, 2012;Alvarenga et al, 2012;Rend on-Anaya et al, 2012;Abdel-Rahman et al, 2013;He et al, 2013;Luna-Ramírez et al, 2013;Valdez-Vel azquez et al, 2013). However, no large-scale effort has been carried out for T. bahiensis and only a few reports of sequencing specific toxins are found in the literature (Becerril et al, 1996(Becerril et al, , 1997Trequattrini et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

The transcriptome recipe for the venom cocktail of Tityus bahiensis scorpion

Oliveira

Cândido

Dorce

et al. 2015

Toxicon

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Scorpion venom is a mixture of peptides, including antimicrobial, bradykinin-potentiating and anionic peptides and small to medium proteins, such as ion channel toxins, metalloproteinases and phospholipases that together cause severe clinical manifestation. Tityus bahiensis is the second most medically important scorpion species in Brazil and it is widely distributed in the country with the exception of the North Region. Here we sequenced and analyzed the transcripts from the venom glands of T. bahiensis, aiming at identifying and annotating venom gland expressed genes. A total of 116,027 long reads were generated by pyrosequencing and assembled in 2891 isotigs. An annotation process identified transcripts by similarity to known toxins, revealing that putative venom components represent 7.4% of gene expression. The major toxins identified are potassium and sodium channel toxins, whereas metalloproteinases showed an unexpected high abundance. Phylogenetic analysis of deduced metalloproteinases from T. bahiensis and other scorpions revealed a pattern of ancient and intraspecific gene expansions. Other venom molecules identified include antimicrobial, anionic and bradykinin-potentiating peptides, besides several putative new venom components. This report provides the first attempt to massively identify the venom components of this species and constitutes one of the few transcriptomic efforts on the genus Tityus.

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Section: Introductionmentioning

confidence: 99%

The transcriptome recipe for the venom cocktail of Tityus bahiensis scorpion

Oliveira

Cândido

Dorce

et al. 2015

Toxicon

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“…One such area of investigation studies the peptides present in the venom of species such as the venomous snakes and scorpions and also the bioactive peptides released in skin secretions from organisms such as New World Frogs (Rates et al, 2008;Conlon et al, 2009). Again, size fractionation and reverse phase fractionation are commonly utilized as initial separation methods for sample cleanup followed by HPLC separation of the samples and mass spectrometric analysis.…”

Section: Toxic and Biologically Active Peptide Analysismentioning

confidence: 99%

Targeted metabolomics and mass spectrometry

Dudley

Yousef

Wang

et al. 2010

Advances in Protein Chemistry and Structural Biology

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“…Proteomic, peptidomic, transcriptomic and functional studies have revealed that TsV possesses neurotoxins, metallopeptidases, hyaluronidase, biogenic amines and peptides, some of which have yet to be isolated and sequenced and/or have unknown function [4][5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…The scientific community has shown an increasing interest in the study of such small peptides present in animal venoms because of their evolutionarily preserved characteristics, which point to the existence of important functional targets (see [14] for a review). Nevertheless, only a limited number of small peptide sequences (<3 kDa) in TsV have been determined thus far, even with the use of sensitive mass spectrometric techniques [6,10,15].…”

Section: Introductionmentioning

confidence: 99%