Tityus serrulatus scorpion venom improves survival and lung inflammation in lethal sepsis induced by CLP in mice

Maciel, Márcia C.G.; Fialho, Eder Magalhães Silva; Guerra, Rosane Nassar Meireles; Borges, Valéria M.; Kwasniewski, Fábio Henrique; Nascimento, Flávia R.F.

doi:10.1016/j.toxicon.2014.06.018

Cited by 17 publications

(6 citation statements)

References 41 publications

(40 reference statements)

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“…[ 24 ] In a recent study, IL‐10 production has been demonstrated to show a protective effect against lethality in a CLP‐induced sepsis model. [ 25 ] Our study results also showed that IL‐10 levels increased in the groups treated with apigenin and reduced the inflammatory effect caused by sepsis. In addition, in the conclusion of our results consistent with the current literature, the administration of apigenin in the case of severe sepsis was found to be an important factor in regulating cytokine responses.…”

Section: Discussionsupporting

confidence: 69%

Investigation of oxidant/antioxidant and anti‐inflammatory effects of apigenin on apoptosis in sepsis‐induced rat lung

Çiçek

Ünsal

Doğaner

et al. 2021

J Biochem & Molecular Tox

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We suppose that apigenin may inhibit the cellular process of sepsis‐induced lung injury, which is considered to be a major cause of morbidity and mortality, and may improve inflammation and oxidative stress. The aim of this study was to investigate the potential protective effect of apigenin in a rat model of polymicrobial sepsis. Eight groups consisting of a total of 64 female Wistar albino rats were used for this study. Pro‐inflammatory (TNF‐α, IL‐1‐β, IL‐6) and anti‐inflammatory (TGF‐β, IL‐10) cytokine levels were measured with the enzyme‐linked immunosorbent assay technique, oxidant/antioxidants parameters were measured using the spectrophotometric method and Bax and Caspase‐3 immunohistochemical methods. TNF‐α, TGF‐β, IL‐1β, and IL‐6 levels significantly increased in the sepsis‐induced group than in the control groups, while IL‐10 levels decreased. Lipid peroxidase (LPO), an oxidative stress marker, increased, while the antioxidant defense parameters of superoxide dismutase (SOD), catalase (CAT) activities, glutathione (GSH) levels decreased. Although Bax and Caspase‐3 immunoreactivity and H score levels significantly increased in the sepsis group, significant decreases were found in the groups treated with apigenin. In conclusion, we are of the opinion that apigenin treatment improves lung injury by inhibiting oxidative stress and inflammatory cell damage.

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Section: Discussionsupporting

confidence: 69%

Investigation of oxidant/antioxidant and anti‐inflammatory effects of apigenin on apoptosis in sepsis‐induced rat lung

Çiçek

Ünsal

Doğaner

et al. 2021

J Biochem & Molecular Tox

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“…IL-6 and MCP-1 presented the highest values in both serum and peritoneal lavage of the F5 group. Other studies have also reported a high production of cytokines in the context of sepsis, such as IL-6, as well as the chemokine MCP-1 [42,43]. The exacerbated cytokines production is indicative of poor prognosis in patients with sepsis, being associated with a higher mortality rate.…”

Section: Discussionmentioning

confidence: 95%

“…The mice were euthanized with an overdose of the anesthetic (150 mg/kg ketamine hydrochloride and 120 mg/kg xylazine hydrochloride), and another half of the animals were maintained alive to evaluate the lifespan. The survival of the animals was evaluated every 12 h until the 5th day after bacteria inoculation [42].…”

Section: Experimental Designmentioning

confidence: 99%

Pic-Producing Escherichia coli Induces High Production of Proinflammatory Mediators by the Host Leading to Death by Sepsis

Dutra

Araújo

Assunção

et al. 2020

IJMS

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Escherichia coli is an important pathogen responsible for a variety of diseases. We have recently shown that Pic, a serine protease secreted by E. coli, mediates immune evasion by the direct cleavage of complement molecules. The aim of this study was to investigate the action of a Pic-producing bacteria in a murine model of sepsis. Mice were infected with Pic-producing E. coli (F5) or F5∆pic mutant. Animal survival was monitored for five days, and a subset of mice was euthanized after 12 h for sample acquisition. The inoculation of Pic-producing bacteria induced 100% death within 24 h. The colony forming units count in the organs was significantly higher in F5. Hematological analysis showed a decrease of total leukocytes. Nitric oxide and cytokines were detected in serum, as well as on peritoneal lavage of the F5 group in higher levels than those detected in the other groups. In addition, immunophenotyping showed a decrease of activated lymphocytes and macrophages in the F5 group. Therefore, Pic represents an important virulence factor, allowing the survival of the bacterium in the bloodstream and several organs, as well as inducing a high production of proinflammatory mediators by the host, and concomitantly a cellular immunosuppression, leading to sepsis and death.

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“…The cytometric bead array (CBA) technique was used for the quantification of TNF-α, MCP-1, IL-6, IL-10, IL-12, and IFN-γ in serum, as described by Maciel et al ( 17 ), using the mouse inflammation cytokine kit (Becton Dickinson Biosciences, San Jose, CA, EUA).…”

Section: Methodsmentioning

confidence: 99%

Prophylactic Treatment With Simvastatin Modulates the Immune Response and Increases Animal Survival Following Lethal Sepsis Infection

et al. 2018

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Chronic use of statins may have anti-inflammatory action, promoting immunomodulation and survival in patients with sepsis. This study aimed to analyze the effects of pretreatment with simvastatin in lethal sepsis induced by cecal ligation and puncture (CLP). Male Swiss mice received prophylactic treatment with simvastatin or pyrogen-free water orally in a single daily dose for 30 days. After this period, the CLP was performed. Naïve and Sham groups were performed as non-infected controls. Animal survival was monitored for 60 h after the CLP. Half of mice were euthanized after 12 h to analyze colony-forming units (CFUs); hematological parameters; production of IL-10, IL-12, IL-6, TNF-α, IFN-γ, and MCP-1; cell counts on peritoneum, bronchoalveolar lavage (BAL), bone marrow, spleen, and mesenteric lymph node; immunephenotyping of T cells and antigen presenting cells and production of hydrogen peroxide (H2O2). Simvastatin induced an increase in survival and a decrease in the CFU count on peritoneum and on BAL cells number, especially lymphocytes. There was an increase in the platelets and lymphocytes number in the Simvastatin group when compared to the CLP group. Simvastatin induced a greater activation and proliferation of CD4+ T cells, as well as an increase in IL-6 and MCP-1 production, in chemotaxis to the peritoneum and in H2O2 secretion at this site. These data suggest that simvastatin has an impact on the survival of animals, as well as immunomodulatory effects in sepsis induced by CLP in mice.

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Tityus serrulatus scorpion venom improves survival and lung inflammation in lethal sepsis induced by CLP in mice

Cited by 17 publications

References 41 publications

Investigation of oxidant/antioxidant and anti‐inflammatory effects of apigenin on apoptosis in sepsis‐induced rat lung

Investigation of oxidant/antioxidant and anti‐inflammatory effects of apigenin on apoptosis in sepsis‐induced rat lung

Pic-Producing Escherichia coli Induces High Production of Proinflammatory Mediators by the Host Leading to Death by Sepsis

Prophylactic Treatment With Simvastatin Modulates the Immune Response and Increases Animal Survival Following Lethal Sepsis Infection

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