Titrimetric and spectrophotometric determination of doxycycline hyclate using bromate-bromide, methyl orange and indigo carmine

Jagannathamurthy, Pavagada; Basavaiah, K.; Ranganath, M. K.; Nagaraju, D.; Kanakapura, Rajendraprasad; Vinay, Basavaiah Kanakapura

doi:10.2298/ciceq091208020r

Cited by 5 publications

(2 citation statements)

References 24 publications

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“…Doxycycline is official in Indian Pharmacopoeia [2], British Pharmacopeia [3], United state Pharmacopeia [4], European Pharmacopeia [5], and Japanese Pharmacopeia [6]. From the literature review, it is observed that many analytical methods have been developed involving potentiometric [7], Differential pulse polarography [8], spectrophotometric [9–11], HPLC [12–15], and LC‐MS [16–17] for the estimation of DOX in pharmaceutical formulations. ORN Figure 2, chemically 1‐chloro‐3‐(2‐methyl‐5‐nitroimidazol‐lyl)Propan‐2‐ol [34].…”

Section: Introductionmentioning

confidence: 99%

Chromatographic separation to determine doxycycline monohydrate and ornidazole

Patel

2023

Separation Science Plus

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High‐performance thin‐layer chromatography offers a variety of benefits that may assist you to save time and money, notably the capacity to run multiple samples at once and its reduced solvent usage. In alignment with the needs of molecular chemistry, it was also employed to modify and derivatize the stationary phase. Using a precise, accurate, as well as affordable high‐performance thin‐layer chromatography approach, the simultaneous measurement method for Doxycycline monohydrate and Ornidazole in the formulation has been developed and validated. Methanol: Iso‐propyl alcohol: Triethylamine (8:1.5:0.5, v/v/v) was used as the mobile phase during the separation, which took place on a stationary phase of pre‐treated derivatized silica gel aluminum plate 60 F254. At 285 nm, separated components were densitometrically measured. The method was validated for Linearity and range, precision, reproducibility, accuracy, the limit of detection, the limit of quantification, and robustness as per the International Council for Harmonization Q2(R1) guideline. Doxycycline monohydrate and Ornidazole regression coefficients (R2) were found to be 0.9967 and 0.9964, accordingly. Doxycycline monohydrate and Ornidazole percentage recoveries were measured to be 99.74 ± 0.65 and 100.8 ± 1.17, respectively. The analytical findings demonstrate that the proposed approach could be used to quantify Doxycycline monohydrate and Ornidazole simultaneously in pharmaceutical formulation.

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Section: Introductionmentioning

confidence: 99%

Chromatographic separation to determine doxycycline monohydrate and ornidazole

Patel

2023

Separation Science Plus

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“…Both ciprofloxacin and doxycycline hyclate are official in the British and United States Pharmacopoeia, and their official method of assay is high-performance liquid chromatography (HPLC) [1, 3]. Several analytical methods such as spectrophotometry [4, 5], fluorimetry [6], ultra-performance liquid chromatographic [7], HPLC [8], high-performance thin-layer chromatography [9], high-performance capillary electrophoresis [10], and capillary electrophoresis [11] have been reported for the determination of ciprofloxacin while spectrophotometry [12, 13] and HPLC [14, 15] are for the determination of doxycycline hyclate alone or in combination with other medicines.…”

Section: Introductionmentioning

confidence: 99%

Chemometric-Assisted Spectrophotometric Method for the Simultaneous Determination of Ciprofloxacin and Doxycycline Hyclate in Pharmaceutical Formulations

Eticha

Kahsay

Asefa

et al. 2018

Journal of Analytical Methods in Chemistry

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Two chemometrics methods—principal component regression and partial least squares—were developed for simultaneous spectrophotometric estimation of ciprofloxacin and doxycycline hyclate in pharmaceutical dosage forms without any pretreatment. The UV spectra of both drugs were recorded at concentrations within their linear ranges between 200 and 400 nm with the intervals λ = 2 nm at 100 wavelengths in distilled water. Beer's law was obeyed for both drugs in the concentration ranges of 1–10 μg/mL for ciprofloxacin and 5–25 μg/mL for doxycycline hyclate. Two sets of standard mixtures, 25 as a calibration set and 9 as a validation set, were prepared. The calibration models were evaluated by cross-validation and external validation over synthetic mixtures. The optimized models were successfully applied for chemometric analysis of ciprofloxacin and doxycycline hyclate in synthetic and pharmaceutical mixtures with satisfactory accuracy (recovery values from 97.50% to 101.87%) and precision (RSD < 2%).

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Development and Validation of Spectrophotometric Methods for the Quantitative Determination of Doxycycline Hyclate in Pure Form and Pharmaceutical Formulations Using Flow‐Injection and Batch procedures: A Comparative Study

Abdulghani

Hassan

2023

ChemistrySelect

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Doxycycline hyclate is an antibiotic drug with a broad‐spectrum activity against a variety of gram‐positive and gram‐negative bacteria and is frequently used as a pharmacological agent and as an effector molecule in an inducible gene expression system. A sensitive, reliable and fast spectrophotometric method for the determination of doxycycline hyclate in pure and pharmaceutical formulations has been developed using flow injection analysis (FIA) and batch procedures. The proposed method is based on the reaction between the chromogenic reagent (V4+) and doxycycline hyclate in a neutral medium, resulting in the formation of a yellow compound that shows maximum absorbance at 396 nm. In a batch procedure, the proposed method was validated over the concentration range of 1.0–80 μg mL−1 with a sampling frequency of 30/h, and commercial pharmaceutical samples were successfully determined. The proposed method was successfully adapted with an FIA system where the peak heights are proportionally connected to doxycycline hyclate over the concentration range of 25–400 μg mL−1 with a sampling frequency of 50/h. The limits of detection (LOD) and quantification (LOQ) were 0.9 and 10.44 μg mL−1 and 3.01 and 34.81 μg mL−1 for batch and FIA respectively. The samples were submitted to an HPLC analysis, and the outcomes demonstrated excellent agreement with the suggested procedures. The adopted FIA procedure allows fast monitoring of doxycycline hyclate in pharmaceutical formulations and it can be used for quality control purposes during the production processes of doxycycline hyclate.

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Titrimetric and spectrophotometric determination of doxycycline hyclate using bromate-bromide, methyl orange and indigo carmine

Cited by 5 publications

References 24 publications

Chromatographic separation to determine doxycycline monohydrate and ornidazole

Chromatographic separation to determine doxycycline monohydrate and ornidazole

Chemometric-Assisted Spectrophotometric Method for the Simultaneous Determination of Ciprofloxacin and Doxycycline Hyclate in Pharmaceutical Formulations

Development and Validation of Spectrophotometric Methods for the Quantitative Determination of Doxycycline Hyclate in Pure Form and Pharmaceutical Formulations Using Flow‐Injection and Batch procedures: A Comparative Study

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