Titrations of Various Mouse Mammary Tumor Viruses in Different Mouse Strains
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Moore, Dan H.; Charney, Jesse; Holben, Jane A.

doi:10.1093/jnci/52.6.1757

Cited by 25 publications

(14 citation statements)

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“…The similarity in nucleic acid sequences of the horizontally transmitted MMTVs of the mouse strains RIII, GR, CMH, and A is reflected in the similarity of virulence and antigenic composition (3,(24)(25)(26)(27). Of particular interest is the similarity in nucleic acid sequence of the MMTV(GR), which is transmitted by both milk and gametes (2), to the horizontally transmitted MMTVs of the other mouse strains studied, and the difference in the genomes of the C3H horizontally and vertically transmitted MMTVs.…”

Section: Competition Hybridizationmentioning

confidence: 99%

“…Variants of the mouse mammary tumor virus (MMTV) have been identified by different biological characteristics such as virulence, host range, mode of transmission, and histological type of tumor induced (1)(2)(3)(4)(5). Proviral sequences of MMTV have been reported (6) to be uniformly omnipresent in the cellular genome of mouse strains with high and low mammary tumor incidence, and to be expressed in all tissues of mouse strains irrespective of their mammary tumor history (7).…”

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confidence: 99%

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Relationship in nucleic acid sequences between mouse mammary tumor virus variants.

Michalides¹,

Schlom²

1975

Proc. Natl. Acad. Sci. U.S.A.

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Primary cultures of mouse mammary carcinomas were used as a source of both radioactively labeled and unlabeled 60 Variants of the mouse mammary tumor virus (MMTV) have been identified by different biological characteristics such as virulence, host range, mode of transmission, and histological type of tumor induced (1-5). Proviral sequences of MMTV have been reported (6) to be uniformly omnipresent in the cellular genome of mouse strains with high and low mammary tumor incidence, and to be expressed in all tissues of mouse strains irrespective of their mammary tumor history (7). Those studies, however, were conducted with cDNA probes that did not represent the entire viral genome (6), and did not consider a possible difference in nucleic acid sequences between vertically and horizontally transmitted MMTVs. We report here, using the 60-70S RNAs of various MMTVs in competitive and direct hybridization studies, that the nucleic acid sequences of the horizontally transmitted MMTV variants from four mouse strains (RIII, C3H-AvY, GR, and A) are at least 95% homologous to one another, and are approximately 25% different from the vertically transmitted MMTV variant from the mouse strain C3H-AVYfB. No Isolation of Nucleic Acids. We isolated radioactively labeled 60-70S RNA from MMTVs as described previously (11,12). Unlabeled virus was purified by polyethylene glycol precipitation (12) and isopycnic sucrose centrifugation. RNA was extracted by use of the sodium dodecyl sulfate Pronase method followed by phenol extraction (11,12). Cellular DNA, isolated (13) and sheared by sonication resulted in DNA fragments with a sedimentation value of 6-8 S, as assayed by alkaline sucrose gradient sedimentation.Hybridization Conditions. A modification of the method used by Wright and Neiman (14) was used for molecular competition hybridizations. The hybridization mixtures, in 100 gl, contained 400 ,ug of cellular DNA fragments, 3H-or 32P-labeled 60-70S MMTV RNA as indicated, and increasing amounts of unlabeled RNA in hybridization mixture I [4 4635

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Section: Competition Hybridizationmentioning

confidence: 99%

mentioning

confidence: 99%

Relationship in nucleic acid sequences between mouse mammary tumor virus variants.

Michalides¹,

Schlom²

1975

Proc. Natl. Acad. Sci. U.S.A.

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“…In particular, RIIIfC57BL neonates have to be nursed for 4 days on RIII mothers in order to obtain 80~o of infected adult females, i.e. producing MMTV in their mammary glands (Moore et al, 1974). Moreover, the susceptibility of a given mouse strain to some MMTV variants may depend on the route of inoculation, whether it is carried out by foster-nursing or by intraperitoneal injection into 4-to 5-week-old animals (Hageman et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

Peroral Infection of Suckling Mice with Milk-borne Mouse Mammary Tumour Virus: Uptake of the Main Viral Antigens by the Gut

Hainaut

François²,

Calberg-Bacq

et al. 1983

Journal of General Virology

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SUMMARYPersistence of mouse mammary tumour virus (MMTV) components in the digestive tract of suckling mice was investigated by immunoperoxidase staining of the main viral antigens and micra-immunoenzyme assays of gp52 and p28; these latter assays were also performed after ingestion of milk enriched in viral antigens using Cr20 3 as a marker for the alimentary bolus migration. When compared to the ingested antigens, the amounts Of gp52 and p28 decreased during transit, p28 being more rapidly digested than gp52. The antigens were, however, destroyed to a much larger extent in the gut of the adult than in that of the newborn mouse. A fraction of the marker remained for a long time in the stomach; a prolonged retention was also observed with gp52 and especially with p28. Significant amounts of viral antigens were detected in the intestinal walls: both p28 and gp52 were found in the duodenum and small intestine. Moreover, the four viral antigens gp52, gp36, p28 and p8 were clearly observed in very large supranuclear vacuoles inside the epithelial cells of the distal part of the gut. Total particles can reach the intestine; the viral material could then be either destroyed or taken up in the epithelial cells by endocytosis, so that the intestinal epithelium might serve as a portal of entry for MMTV in the suckling mouse.

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“…Differences in mouse mammary tumor viruses (MMTVs) have been widely accepted with respect to biological activities, host range, or modes of transmission (1)(2)(3). More recently, nucleic acid hybridization studies have demonstrated genomic differences between various MMTVs (4,5).…”

mentioning

confidence: 99%

Multiple antigenic determinants on the major surface glycoprotein of murine mammary tumor viruses.

Teramoto¹,

Küfe²,

Schlom³

1977

Proc. Natl. Acad. Sci. U.S.A.

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The major external protein of murine mammary tumor viruses (MMWVs) is a glycoprotein of molecular weight 52,000 (gp52). We report here that the gp52s of MMTVs from different mouse strains contain both type-specific and group-specific antigenic determinants. Competitive radioimmunoassays with externally labeled virion preparations were used to demonstrate that MMTV virions from C3H, RIII, and GR mice competed identically in "group-specific" assays but either competed with altered slopes or competed incompletely in the appropriate "type-specific" assays. Similar results were obtained with purified gp52 preparations as competitors.MMTVs of RIII, C3H, and GR mice, grown in felin? cells, competed similarly to the corresponding murineidrived MMTVs, thus demonstrating that the observed type-specific and group-specific antigenic reactivities are viral coded. In competitive radioimmunoassays using an antiserum against MMTV of C3H mice that was preabsorbed with MMTV of GR mice, type-specific reactivities were further magnified; MMTV of C3H mice competed completely whereas no competition was observed with MMTV of GIR mice as a competitor. These studies are in agreement with previous biological and molecular hybridization studies on the diversity of MMTVs from different mouse strains.Differences in mouse mammary tumor viruses (MMTVs) have been widely accepted with respect to biological activities, host range, or modes of transmission (1-3). More recently, nucleic acid hybridization studies have demonstrated genomic differences between various MMTVs (4, 5).Immunological assays including virus neutralization (6), immunization (7), and immunodiffusion (8) studies have indicated antigenic differences among MMTVs isolated from different strains of mice. These serological data are difficult to interpret, however, principally due to the lack of pure cell culture sources of MMTV virions and MMTV proteins, free of contaminating cellular debris, as well as to the lack of MMTVs grown in nonmurine cells.Highly purified preparations of MMTVs have beei obtained from primary cultures of mouse mammary tumor cells and from mouse mammary tumor cell lines. The polypeptide and antigenic structures of MMTVs purified from culture fluids have been described (9, 10). The major surface component of the MMTV virion envelope is a 52,000-dalton glycoprotein (gp52). The other major protein components of the MMTV virion are a 36,000-dalton glycoprotein (gp36), and polypeptides of 28,000 (p28), 14,000 (p14), and 10,000 (plO) daltons.With the development of sensitive radioimmunoassays for the whole MMTV virion (9) and for purified MMTV polypeptides (11)(12)(13)

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Titrations of Various Mouse Mammary Tumor Viruses in Different Mouse Strains 2

Cited by 25 publications

References 0 publications

Relationship in nucleic acid sequences between mouse mammary tumor virus variants.

Relationship in nucleic acid sequences between mouse mammary tumor virus variants.

Peroral Infection of Suckling Mice with Milk-borne Mouse Mammary Tumour Virus: Uptake of the Main Viral Antigens by the Gut

Multiple antigenic determinants on the major surface glycoprotein of murine mammary tumor viruses.

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