Titrations in nonaqueous solvents

Kratochvil, Byron

doi:10.1021/ac00242a011

Cited by 7 publications

(1 citation statement)

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“…Comparing the obtained results with those calculated according to the molecular weight required by the structures of 1 and 6 , we would have had confirmation of their mass and structure. In this regard, the emergence of non-aqueous titrations in the middle of the last century has enabled the possibility of determining both weak acids and bases not measurable in aqueous media [ 33 , 34 , 35 ]. Especially the titration of weak basic drugs with perchloric acid in glacial acetic acid medium is widely used.…”

Section: Resultsmentioning

confidence: 99%

Anti Gram-Positive Bacteria Activity of Synthetic Quaternary Ammonium Lipid and Its Precursor Phosphonium Salt

Bacchetti,

Schito,

Milanese

et al. 2024

IJMS

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Organic ammonium and phosphonium salts exert excellent antimicrobial effects by interacting lethally with bacterial membranes. Particularly, quaternary ammonium lipids have demonstrated efficiency both as gene vectors and antibacterial agents. Here, aiming at finding new antibacterial devices belonging to both classes, we prepared a water-soluble quaternary ammonium lipid (6) and a phosphonium salt (1) by designing a synthetic path where 1 would be an intermediate to achieve 6. All synthesized compounds were characterized by Fourier-transform infrared spectroscopy and Nuclear Magnetic Resonance. Additionally, potentiometric titrations of NH3+ groups 1 and 6 were performed to further confirm their structure by determining their experimental molecular weight. The antibacterial activities of 1 and 6 were assessed first against a selection of multi-drug-resistant clinical isolates of both Gram-positive and Gram-negative species, observing remarkable antibacterial activity of both compounds against Gram-positive isolates of Enterococcus and Staphylococcus genus. Further investigations on a wider variety of strains of these species confirmed the remarkable antibacterial effects of 1 and 6 (MICs = 4–16 and 4–64 µg/mL, respectively), while 24 h-time-killing experiments carried out with 1 on different S. aureus isolates evidenced a bacteriostatic behavior. Moreover, both compounds 1 and 6, at the lower MIC concentration, did not show significant cytotoxic effects when exposed to HepG2 human hepatic cell lines, paving the way for their potential clinical application.

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Section: Resultsmentioning

confidence: 99%