Title: Supercomplex formed by the cross-binding of multiple antibody-single repeat chain complex gives signal amplification of antibody based assay

Abstract

It is a challenging subject of biomedical research to develop more efficient and sensitive assay method. New method for enhancing sensitivity and precision of conventional immunological assays is developed. The antibody binding domain of Streptococcus protein G was used to make a chain of repeated antibody binding domain. The repeat chain was mixed with antibody, and multiple number of antibody bound to the repeat chain to form multiple antibody-repeat chain complex. The cross-binding between the complexes formed supercomplex, and the supercomplex amplified signals without specificity loss and background noise increase.