Titanium(IV) oxide nanoparticles functionalized with various meso-porphyrins for efficient photocatalytic degradation of ibuprofen in UV and visible light

Krakowiak, Rafał; Frankowski, Robert; Mylkie, Kinga; Kotkowiak, Michał; Mlynarczyk, Dariusz T.; Dudkowiak, Alina; Stanisz, Beata; Zgoła‐Grześkowiak, Agnieszka; Ziegler-Borowska, Marta; Gośliński, Tomasz

doi:10.1016/j.jece.2022.108432

Cited by 17 publications

(3 citation statements)

References 76 publications

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Section: Discussionmentioning

confidence: 99%

Spectral Characterization of a Blue Light-Emitting Micro-LED Platform and Microbial Chromophores for Therapeutic Applications in Skin Conditions

Serrage,

Eling,

Alves

et al. 2024

Preprint

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The therapeutic application of blue light (380 to 500nm) has garnered considerable attention in recent years as it offers a non invasive approach for the management of prevalent skin conditions including acne vulgaris and atopic dermatitis. These conditions are often characterised by an imbalance in the microbial communities that colonise our skin, termed the skin microbiome. In conditions including acne vulgaris, blue light is thought to address this imbalance through the selective photoexcitation of microbial species expressing wavelength-specific chromophores, differentially affecting skin commensals and thus altering the relative species composition. However, the abundance and diversity of these chromophores across the skin microbiota remains poorly understood. Similarly, devices utilised for studies are often bulky and poorly characterised which if translated to therapy could result in reduced patient compliance. Here, we present a clinically viable micro-LED illumination platform with peak emission 450 nm (17 nm FWHM) and adjustable irradiance output to a maximum 0.55 W/cm2, dependent upon the concentration of titanium dioxide nanoparticles applied to an accompanying flexible light extraction substrate. Utilising spectrometry approaches, we characterised the abundance of prospective blue light chromophores across skin commensal bacteria isolated from healthy volunteers. Of the strains surveyed 62.5% exhibited absorption peaks within the blue light spectrum, evidencing expression of carotenoid pigments (18.8%, 420 to 483 nm; Micrococcus luteus, Kocuria spp.), porphyrins (12.5%, 402 to 413 nm; Cutibacterium spp.) and potential flavins (31.2%, 420 to 425 nm; Staphylococcus and Dermacoccus spp.). We also present evidence of the capacity of these species to diminish irradiance output when combined with the micro-LED platform and in turn how exposure to low dose blue light causes shifts in observed absorbance spectra peaks. Collectively these findings highlight a crucial deficit in understanding how microbial chromophores might shape response to blue light and in turn evidence of a micro-LED illumination platform with potential for clinical applications.

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Section: Discussionmentioning

confidence: 99%

Spectral Characterization of a Blue Light-Emitting Micro-LED Platform and Microbial Chromophores for Therapeutic Applications in Skin Conditions

Serrage,

Eling,

Alves

et al. 2024

Preprint

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show abstract

“…It also offers a dual advantage, in which photocatalysis of TiO 2 has proven to induce the production of reactive oxygen species (ROS). This property may prove beneficial for potential decontamination applications [ 40 ]. Photoactivated titanium nanoparticles are now being introduced into paints and coatings to decontaminate hospital surfaces [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

Spectral characterization of a blue light-emitting micro-LED platform on skin-associated microbial chromophores

Serrage,

Eling,

Alves

et al. 2024

Biomed. Opt. Express

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The therapeutic application of blue light (380 – 500nm) has garnered considerable attention in recent years as it offers a non-invasive approach for the management of prevalent skin conditions including acne vulgaris and atopic dermatitis. These conditions are often characterised by an imbalance in the microbial communities that colonise our skin, termed the skin microbiome. In conditions including acne vulgaris, blue light is thought to address this imbalance through the selective photoexcitation of microbial species expressing wavelength-specific chromophores, differentially affecting skin commensals and thus altering the relative species composition. However, the abundance and diversity of these chromophores across the skin microbiota remains poorly understood. Similarly, devices utilised for studies are often bulky and poorly characterised which if translated to therapy could result in reduced patient compliance. Here, we present a clinically viable micro-LED illumination platform with peak emission 450 nm (17 nm FWHM) and adjustable irradiance output to a maximum 0.55 ± 0.01 W/cm2, dependent upon the concentration of titanium dioxide nanoparticles applied to an accompanying flexible light extraction substrate. Utilising spectrometry approaches, we characterised the abundance of prospective blue light chromophores across skin commensal bacteria isolated from healthy volunteers. Of the strains surveyed 62.5% exhibited absorption peaks within the blue light spectrum, evidencing expression of carotenoid pigments (18.8%, 420–483 nm; Micrococcus luteus, Kocuria spp.), porphyrins (12.5%, 402–413 nm; Cutibacterium spp.) and potential flavins (31.2%, 420–425 nm; Staphylococcus and Dermacoccus spp.). We also present evidence of the capacity of these species to diminish irradiance output when combined with the micro-LED platform and in turn how exposure to low-dose blue light causes shifts in observed absorbance spectra peaks. Collectively these findings highlight a crucial deficit in understanding how microbial chromophores might shape response to blue light and in turn evidence of a micro-LED illumination platform with potential for clinical applications.

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“…The supernatant was removed, and the precipitate was resuspended in distilled water. The entire protocol was performed according to the literature [24][25][26].…”

Section: Chemical Compoundsmentioning

confidence: 99%

Photodynamic Activity of TMPyP4/TiO2 Complex under Blue Light in Human Melanoma Cells: Potential for Cancer-Selective Therapy

et al. 2023

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The combination of TiO2 nanoparticles (NPs) and photosensitizers (PS) may offer significant advantages in photodynamic therapy (PDT) of melanoma, such as improved cell penetration, enhanced ROS production, and cancer selectivity. In this study, we aimed to investigate the photodynamic effect of 5,10,15,20-(Tetra-N-methyl-4-pyridyl)porphyrin tetratosylate (TMPyP4) complexes with TiO2 NPs on human cutaneous melanoma cells by irradiation with 1 mW/cm2 blue light. The porphyrin conjugation with the NPs was analyzed by absorption and FTIR spectroscopy. The morphological characterization of the complexes was performed by Scanning Electron Microscopy and Dynamic Light Scattering. The singlet oxygen generation was analyzed by phosphorescence at 1270 nm. Our predictions indicated that the non-irradiated investigated porphyrin has a low degree of toxicity. The photodynamic activity of the TMPyP4/TiO2 complex was assessed on the human melanoma Mel-Juso cell line and non-tumor skin CCD-1070Sk cell line treated with various concentrations of the PS and subjected to dark conditions and visible light-irradiation. The tested complexes of TiO2 NPs with TMPyP4 presented cytotoxicity only after activation by blue light (405 nm) mediated by the intracellular production of ROS in a dose-dependent manner. The photodynamic effect observed in this evaluation was higher in melanoma cells than the effect observed in the non-tumor cell line, demonstrating a promising potential for cancer-selectivity in PDT of melanoma.

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Titanium(IV) oxide nanoparticles functionalized with various meso-porphyrins for efficient photocatalytic degradation of ibuprofen in UV and visible light

Cited by 17 publications

References 76 publications

Spectral Characterization of a Blue Light-Emitting Micro-LED Platform and Microbial Chromophores for Therapeutic Applications in Skin Conditions

Spectral Characterization of a Blue Light-Emitting Micro-LED Platform and Microbial Chromophores for Therapeutic Applications in Skin Conditions

Spectral characterization of a blue light-emitting micro-LED platform on skin-associated microbial chromophores

Photodynamic Activity of TMPyP4/TiO2 Complex under Blue Light in Human Melanoma Cells: Potential for Cancer-Selective Therapy

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