Titanium(IV) immobilized affinity chromatography facilitated phosphoproteomics analysis of salivary extracellular vesicles for lung cancer

Wahid, Amir; Sohail, Amir; Wang, Huiyu; Guo, Miao; Zhang, Lu; Ji, Yin; Wang, Peng; Xiao, Hua

doi:10.1007/s00216-022-04013-7

Cited by 6 publications

(3 citation statements)

References 34 publications

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“…The phospholipid membrane of EVs has a negatively charged phosphate group, and there is a strong affinity between positively charged metal ions or metal oxides and negatively charged phosphate groups; such materials have been widely used for phosphopeptide enrichment. 77 Similarly, by using the covalent bonding between a solid phase material and the phosphate group of the EV phospholipid film, EVs can be stably adsorbed on the surface of material, so as to realize isolation of EVs.…”

Section: Material-based Isolation Methods For Evsmentioning

confidence: 99%

Recent research on material-based methods for isolation of extracellular vesicles

Chen,

Li,

Lin

et al. 2024

Anal. Methods

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Section: Material-based Isolation Methods For Evsmentioning

confidence: 99%

Recent research on material-based methods for isolation of extracellular vesicles

Chen,

Li,

Lin

et al. 2024

Anal. Methods

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“…Some bottom-up studies suggested that the analysis of the phospho-proteome of salivary extracellular vesicles could offer a possibility for either the diagnosis of lung cancer [ 88 ] or to distinguish oral squamous cell carcinoma patients from healthy individuals [ 89 ]. Our studies were able to evidence that during the last months of foetal growth, the phosphorylation of secretory salivary proteins, and therefore the activity of Fam20C, is very low, if not completely absent [ 90 ].…”

Section: Phosphorylationmentioning

confidence: 99%

The Post-Translational Modifications of Human Salivary Peptides and Proteins Evidenced by Top-Down Platforms

Messana

Manconi

Cabras

et al. 2023

IJMS

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In this review, we extensively describe the main post-translational modifications that give rise to the multiple proteoforms characterized to date in the human salivary proteome and their potential role. Most of the data reported were obtained by our group in over twenty-five years of research carried out on human saliva mainly by applying a top-down strategy. In the beginning, we describe the products generated by proteolytic cleavages, which can occur before and after secretion. In this section, the most relevant families of salivary proteins are also described. Next, we report the current information concerning the human salivary phospho-proteome and the limited news available on sulfo-proteomes. Three sections are dedicated to the description of glycation and enzymatic glycosylation. Citrullination and N- and C-terminal post-translational modifications (PTMs) and miscellaneous other modifications are described in the last two sections. Results highlighting the variation in the level of some proteoforms in local or systemic pathologies are also reviewed throughout the sections of the manuscript to underline the impact and relevance of this information for the development of new diagnostic biomarkers useful in clinical practice.

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“…A total of 857 unique phosphopeptides corresponding to 721 saliva phosphoproteins were identified. Among the identified phosphorylation sites, 37 sites and 217 sites were, respectively, upregulated and downregulated in patients, holding potential for the early detection of lung cancer via salivary diagnostics [131].…”

Section: Lung Cancermentioning

confidence: 99%

Salivary Extracellular Vesicles: Biomarkers and Beyond in Human Diseases

Wu,

Liu,

Jia

et al. 2023

IJMS

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Extracellular vesicles, as bioactive molecules, have been extensively studied. There are abundant studies in the literature on their biogenesis, secretion, structure, and content, and their roles in pathophysiological processes. Extracellular vesicles have been reviewed as biomarkers for use in diagnostic tools. Saliva contains many extracellular vesicles, and compared with other body fluids, it is easier to obtain in a non-invasive way, making its acquisition more easily accepted by patients. In recent years, there have been numerous new studies investigating the role of salivary extracellular vesicles as biomarkers. These studies have significant implications for future clinical diagnosis. Therefore, in this paper, we summarize and review the potential applications of salivary extracellular vesicles as biomarkers, and we also describe their other functions (e.g., hemostasis, innate immune defense) in both oral and non-oral diseases.

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Titanium(IV) immobilized affinity chromatography facilitated phosphoproteomics analysis of salivary extracellular vesicles for lung cancer

Cited by 6 publications

References 34 publications

Recent research on material-based methods for isolation of extracellular vesicles

Recent research on material-based methods for isolation of extracellular vesicles

The Post-Translational Modifications of Human Salivary Peptides and Proteins Evidenced by Top-Down Platforms

Salivary Extracellular Vesicles: Biomarkers and Beyond in Human Diseases

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