Titanium dioxide nanoparticles induce an adaptive inflammatory response and invasion and proliferation of lung epithelial cells in chorioallantoic membrane

Medina‐Reyes, Estefany I.; Déciga-Alcaraz, Alejandro; Freyre-Fonseca, Verónica; Delgado-Buenrostro, Norma L.; Flores-Flores, José O.; Gutiérrez-López, Gustavo F.; Sánchez-Pérez, Yesennia; García-Cuéllar, Claudia M.; Pedraza‐Chaverrí, José; Chirino, Yolanda I.

doi:10.1016/j.envres.2014.10.016

Cited by 23 publications

(12 citation statements)

References 52 publications

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“…24 In addition, it has been shown that titanium dioxide NP result in an inflammatory cytokine release in lung epithelial cells. 25 Unlike these nanosystems, HYA-PHMB-NC and PLLA-OCT-NP do not induce a significant inflammatory response. These findings in the present study indicate that vascular inflammatory processes during wound healing will probably not be enhanced or generally interfered with by the presence of HYA-PHMB-NC and PLLA-OCT-NP.…”

Section: Discussionmentioning

confidence: 99%

Enzyme-responsive nanocomposites for wound infection prophylaxis in burn management: in vitro evaluation of their compatibility with healing processes

Grützner

Unger

Baier³

et al. 2015

IJN

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Responsive, theranostic nanosystems, capable of both signaling and treating wound infections, is a sophisticated approach to reduce the most common and potentially traumatizing side effects of burn wound treatment: slowed wound healing due to prophylactic anti-infective drug exposure as well as frequent painful dressing changes. Antimicrobials as well as dye molecules have been incorporated into biodegradable nanosystems that release their content only in the presence of pathogens. Following nanocarrier degradation by bacterial enzymes, any infection will thus emit a visible signal and be effectively treated at its source. In this study, we investigated the effect of fluorescent-labeled hyaluronan nanocapsules containing polyhexanide biguanide and poly-L-lactic acid nanoparticles loaded with octenidine on primary human dermal microvascular endothelial cells, which play a major role in cutaneous wound healing. Microscopic and flow cytometric analysis indicated a time-dependent uptake of both the nanocapsules and the nanoparticles. However, enzyme immunoassays showed no significant influence on the expression of pro-inflammatory cell adhesion molecules and cytokines by the endothelial cells. Under angiogenic-stimulating conditions, the potential to form capillary-like structures in co-culture with dermal fibroblasts was not inhibited. Furthermore, cytotoxicity studies (the MTS and crystal violet assay) after short- and long-term exposure to the materials demonstrated that both systems exhibited less toxicity than solutions of the antiseptic agents alone in comparable concentrations. The results indicate that responsive antimicrobial nanocomposites could be used as an advanced drug delivery system and a promising addition to current best practice wound infection prophylaxis with few side effects.

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Section: Discussionmentioning

confidence: 99%

Enzyme-responsive nanocomposites for wound infection prophylaxis in burn management: in vitro evaluation of their compatibility with healing processes

Grützner

Unger

Baier³

et al. 2015

IJN

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“…The morphology of NPs is crucial to their toxicity. The effects of NPs on organisms might be regulated by morphological structure [115][116][117][118][119]. However, how different shapes (mainly including nanobelts, nanorods, nanotubes, and nanospheres) of TiO 2 NPs modulate their transportation to the brain, how they get excretion from the CNS, and how they have toxic effects on neurons or glia cells are still largely unknown.…”

Section: Shape and Surface Modificationmentioning

confidence: 99%

A review on potential neurotoxicity of titanium dioxide nanoparticles

Song¹,

Liu²,

Feng³

et al. 2016

Nano Online

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As the rapid development of nanotechnology in the past three decades, titanium dioxide nanoparticles (TiO 2 NPs), for their peculiar physicochemical properties, are widely applied in consumer products, food additives, cosmetics, drug carriers, and so on. However, little is known about their potential exposure and neurotoxic effects. Once NPs are unintentionally exposed to human beings, they could be absorbed, and then accumulated in the brain regions by passing through the blood-brain barrier (BBB) or through the nose-to-brain pathway, potentially leading to dysfunctions of central nerve system (CNS). Besides, NPs may affect the brain development of embryo by crossing the placental barrier. A few in vivo and in vitro researches have demonstrated that the morphology and function of neuronal or glial cells could be impaired by TiO 2 NPs which might induce cell necrosis. Cellular components, such as mitochondrial, lysosome, and cytoskeleton, could also be influenced as well. The recognition ability, spatial memory, and learning ability of TiO 2 NPs-treated rodents were significantly impaired, which meant that accumulation of TiO 2 NPs in the brain could lead to neurodegeneration. However, conclusions obtained from those studies were not consistent with each other as researchers may choose different experimental parameters, including administration ways, dosage, size, and crystal structure of TiO 2 NPs. Therefore, in order to fully understand the potential risks of TiO 2 NPs to brain health, figure out research areas where further studies are required, and improve its bio-safety for applications in the near future, how TiO 2 NPs interact with the brain is investigated in this review by summarizing the current researches on neurotoxicity induced by TiO 2 NPs.International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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“…Some of these toxicity mechanisms manifest only after prolonged exposure, which is often neglected during short-term in vitro studies but crucial for better understanding of prolonged environmental or medical exposure, which can result in an organ accumulation of NPs [ 11 , 12 , 13 , 14 , 15 ]. However, due to the variety of protocols, lack of standardized physicochemical characterization of NPs [ 3 , 4 ], and only a limited number of long-term studies [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ], there is no general agreement about the potential long-term toxicity of several types of specific NPs. Long-term TiO 2 NPs exposure has been shown to alter proliferation [ 38 ] as well as induce stress [ 38 ] and inflammatory responses [ 19 , 30 , 38 ].…”

Section: Introductionmentioning

confidence: 99%

“…Due to the lack of the appropriate models of differentiating and/or differentiated cells that would more accurately represent the in vivo condition, only a limited number of studies evaluated the long-term cytotoxic effects of NPs [ 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. Thus, the great majority of long-term studies is performed on proliferative cells, which require repeated subcultivation [ 16 , 17 , 18 , 20 , 23 , 26 , 27 , 28 , 29 , 32 , 34 , 35 , 36 , 38 ] or are grown in bioreactors [ 31 , 32 ].…”

Section: Introductionmentioning

confidence: 99%

Proposing Urothelial and Muscle In Vitro Cell Models as a Novel Approach for Assessment of Long-Term Toxicity of Nanoparticles

Skočaj

Bizjak

Strojan

et al. 2020

IJMS

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Many studies evaluated the short-term in vitro toxicity of nanoparticles (NPs); however, long-term effects are still not adequately understood. Here, we investigated the potential toxic effects of biomedical (polyacrylic acid and polyethylenimine coated magnetic NPs) and two industrial (SiO2 and TiO2) NPs following different short-term and long-term exposure protocols on two physiologically different in vitro models that are able to differentiate: L6 rat skeletal muscle cell line and biomimetic normal porcine urothelial (NPU) cells. We show that L6 cells are more sensitive to NP exposure then NPU cells. Transmission electron microscopy revealed an uptake of NPs into L6 cells but not NPU cells. In L6 cells, we obtained a dose-dependent reduction in cell viability and increased reactive oxygen species (ROS) formation after 24 h. Following continuous exposure, more stable TiO2 and polyacrylic acid (PAA) NPs increased levels of nuclear factor Nrf2 mRNA, suggesting an oxidative damage-associated response. Furthermore, internalized magnetic PAA and TiO2 NPs hindered the differentiation of L6 cells. We propose the use of L6 skeletal muscle cells and NPU cells as a novel approach for assessment of the potential long-term toxicity of relevant NPs that are found in the blood and/or can be secreted into the urine.

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Titanium dioxide nanoparticles induce an adaptive inflammatory response and invasion and proliferation of lung epithelial cells in chorioallantoic membrane

Cited by 23 publications

References 52 publications

Enzyme-responsive nanocomposites for wound infection prophylaxis in burn management: in vitro evaluation of their compatibility with healing processes

Enzyme-responsive nanocomposites for wound infection prophylaxis in burn management: in vitro evaluation of their compatibility with healing processes

A review on potential neurotoxicity of titanium dioxide nanoparticles

Proposing Urothelial and Muscle In Vitro Cell Models as a Novel Approach for Assessment of Long-Term Toxicity of Nanoparticles

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Titanium dioxide nanoparticles induce an adaptive inflammatory response and invasion and proliferation of lung epithelial cells in chorioallantoic membrane

Cited by 23 publications

References 52 publications

Enzyme-responsive nanocomposites for wound infection prophylaxis in burn management: in&nbsp;vitro evaluation of their compatibility with healing processes

Enzyme-responsive nanocomposites for wound infection prophylaxis in burn management: in&nbsp;vitro evaluation of their compatibility with healing processes

A review on potential neurotoxicity of titanium dioxide nanoparticles

Proposing Urothelial and Muscle In Vitro Cell Models as a Novel Approach for Assessment of Long-Term Toxicity of Nanoparticles

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Product

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Enzyme-responsive nanocomposites for wound infection prophylaxis in burn management: in vitro evaluation of their compatibility with healing processes

Enzyme-responsive nanocomposites for wound infection prophylaxis in burn management: in vitro evaluation of their compatibility with healing processes