Titanium Dioxide Nanoparticles Alter Pulmonary Surfactant Ultrastructure and Biophysical Function.

Schleh, Carsten; Mühlfeld, Christian; Pulskamp, Karin; Schmiedl, Andreas; Braun, Al.; Nassimi, Matthias; Lauenstein, Hans-Dieter; Krug, Norbert; Erpenbeck, VJ; Hohlfeld, JM

doi:10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a5255

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“…The authors suggest that the sequence of particle and monolayer addition may influence nanoparticle−lipid interactions. 16 Adverse effects of metallic nanoparticle−lung surfactant lipid interactions on the surface-tension-lowering ability was reported for TiO 2 nanoparticles of size ∼5 nm but not for microparticles of TiO 2 , 17,18 suggesting that the size of these metallic nanoparticles contributes to their interactions with lipids. Along similar lines, Kodama et al recently presented evidence of the existence of a critical particle size range that effects the phospholipid domain packing of a model lung surfactant.…”

Section: ■ Introductionmentioning

confidence: 99%

Phospholipid Composition Modulates Carbon Nanodiamond-Induced Alterations in Phospholipid Domain Formation

et al. 2015

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The focus of this work is to elucidate how phospholipid composition can modulate lipid nanoparticle interactions in phospholipid monolayer systems. We report on alterations in lipid domain formation induced by anionically engineered carbon nanodiamonds (ECNs) as a function of lipid headgroup charge and alkyl chain saturation. Using surface pressure vs area isotherms, monolayer compressibility, and fluorescence microscopy, we found that anionic ECNs induced domain shape alterations in zwitterionic phosphatidylcholine lipids, irrespective of the lipid alkyl chain saturation, even when the surface pressure vs area isotherms did not show any significant changes. Bean-shaped structures characteristic of dipalmitoylphosphatidylcholine (DPPC) were converted to multilobed, fractal, or spiral domains as a result of exposure to ECNs, indicating that ECNs lower the line tension between domains in the case of zwitterionic lipids. For membrane systems containing anionic phospholipids, ECN-induced changes in domain packing were related to the electrostatic interactions between the anionic ECNs and the anionic lipid headgroups, even when zwitterionic lipids are present in excess. By comparing the measured size distributions with our recently developed theory derived by minimizing the free energy associated with the domain energy and mixing entropy, we found that the change in line tension induced by anionic ECNs is dominated by the charge in the condensed lipid domains. Atomic force microscopy images of the transferred anionic films confirm that the location of the anionic ECNs in the lipid monolayers is also modulated by the charge on the condensed lipid domains. Because biological membranes such as lung surfactants contain both saturated and unsaturated phospholipids with different lipid headgroup charges, our results suggest that when studying potential adverse effects of nanoparticles on biological systems the role of lipid compositions cannot be neglected.

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Section: ■ Introductionmentioning

confidence: 99%