Titanium dioxide ceramics control the differentiated phenotype of cardiac muscle cells in culture

Polonchuk, Ljudmila; Elbel, Joerg; Eckert, L.; Blum, J.; Wintermantel, E.; Eppenberger, Hans M.

doi:10.1016/s0142-9612(99)00189-1

Cited by 42 publications

(18 citation statements)

References 28 publications

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“…Another cue is a unique surface topography due to the fibrillar structure of FDM compared to very smooth gelatin and fibronectin. Surface topography would provide an advanced microenvironment for maintenance of differentiated state of cardiomyocyte 36 and also a regulatory cue in stem cell differentiation. 37 All of these innate properties of FDM give improved cell behaviors compared to those on gelatin or fibronectin.…”

Section: Discussionmentioning

confidence: 99%

Cardiomyoblast (H9c2) Differentiation on Tunable Extracellular Matrix Microenvironment

Suhaeri

Subbiah

Van

et al. 2015

Tissue Engineering Part A

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Extracellular matrices (ECM) obtained from in vitro-cultured cells have been given much attention, but its application in cardiac tissue engineering is still limited. This study investigates cardiomyogenic potential of fibroblast-derived matrix (FDM) as a novel ECM platform over gelatin or fibronectin, in generating cardiac cell lineages derived from H9c2 cardiomyoblasts. As characterized through SEM and AFM, FDM exhibits unique surface texture and biomechanical property. Immunofluorescence also found fibronectin, collagen, and laminin in the FDM. Cells on FDM showed a more circular shape and slightly less proliferation in a growth medium. After being cultured in a differentiation medium for 7 days, H9c2 cells on FDM differentiated into cardiomyocytes, as identified by stronger positive markers, such as a-actinin and cTnT, along with more elevated gene expression of Myl2 and Tnnt compared to the cells on gelatin and fibronectin. The gap junction protein connexin 43 was also significantly upregulated for the cells differentiated on FDM. A successive work enabled matrix stiffness tunable; FDM crosslinked by 2wt% genipin increased the stiffness up to 8.5 kPa, 100 times harder than that of natural FDM. The gene expression of integrin subunit a5 was significantly more upregulated on FDM than on crosslinked FDM (X-FDM), whereas no difference was observed for b1 expression. Interestingly, X-FDM showed a much greater effect on the cardiomyoblast differentiation into cardiomyocytes over natural one. This study strongly indicates that FDM can be a favorable ECM microenvironment for cardiomyogenesis of H9c2 and that tunable mechanical compliance induced by crosslinking further provides a valuable insight into the role of matrix stiffness on cardiomyogenesis.

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Section: Discussionmentioning

confidence: 99%

Cardiomyoblast (H9c2) Differentiation on Tunable Extracellular Matrix Microenvironment

Suhaeri

Subbiah

Van

et al. 2015

Tissue Engineering Part A

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“…6 This complicated process is commonly referred to as bidirectional signal transduction, in which cellular adhesion and material surface properties correlate intimately. In tissue engineering, the surface properties of biological materials usually impose crucial impacts on cell culture, 7 healing of wounds, 8 and tissue restoration and reconstruction. 9 Specifically, cell behavior can be manipulated by altering their material properties, including chemical, 10,11 nonmechanical physical, 12,13 and mechanical properties in vitro.…”

Section: Introductionmentioning

confidence: 99%

Nanoscale TiO2 nanotubes govern the biological behavior of human glioma and osteosarcoma cells

Tian¹,

Qin²,

Wu³

et al. 2015

IJN

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Cells respond to their surroundings through an interactive adhesion process that has direct effects on cell proliferation and migration. This research was designed to investigate the effects of TiO 2 nanotubes with different topographies and structures on the biological behavior of cultured cells. The results demonstrated that the nanotube diameter, rather than the crystalline structure of the coatings, was a major factor for the biological behavior of the cultured cells. The optimal diameter of the nanotubes was 20 nm for cell adhesion, migration, and proliferation in both glioma and osteosarcoma cells. The expression levels of vitronectin and phosphor-focal adhesion kinase were affected by the nanotube diameter; therefore, it is proposed that the responses of vitronectin and phosphor-focal adhesion kinase to the nanotube could modulate cell fate. In addition, the geometry and size of the nanotube coating could regulate the degree of expression of acetylated α-tubulin, thus indirectly modulating cell migration behavior. Moreover, the expression levels of apoptosis-associated proteins were influenced by the topography. In conclusion, a nanotube diameter of 20 nm was the critical threshold that upregulated the expression level of Bcl-2 and obviously decreased the expression levels of Bax and caspase-3. This information will be useful for future biomedical and clinical applications.

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“…Various attempts were made modifying the speed of the reciprocatory shaker, using glass bed of different size but the results were poor. The choice of titanium dioxide as support for the growth was due to its well-known biocompatibility (Polonchuck et al 2000) and low water solubility that formed a fine suspension. Moreover, in the dark, titanium dioxide has no appreciable toxicity for the cells (Blake et al 1999).…”

Section: Optimization Of Culture Conditions For Biotransformationmentioning

confidence: 99%

Enantioselective Baeyer–Villiger Oxidation of Bicyclo[3.2.0]hept-2-en-6-One with Fungi: Optimization of Biotransformation and Use of TiO2 as Support of Cell Growth

et al. 2006

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Fungi from Amazonian forest soil (Ecuador) and an Italian factory were screened for Baeyer-Villiger (BV) oxidation of bicyclo [3.2.0]hept-2-en-6-one to 2-oxabicyclo[3.3.0]oct-6-en-3-one (Corey's lactone). Isolates of Fusarium sp. and F. solani produced the (+)-(1R,5S)-lactone while isolates of Aspergillus terricola and A. amazonicus afforded the (-)-(1S,5R)-lactone. Highest conversions (85% yield and 70% enantiomeric excess) were obtained with A. amazonicus grown in presence of 2.7 mM titanium dioxide.

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Titanium dioxide ceramics control the differentiated phenotype of cardiac muscle cells in culture

Cited by 42 publications

References 28 publications

Cardiomyoblast (H9c2) Differentiation on Tunable Extracellular Matrix Microenvironment

Cardiomyoblast (H9c2) Differentiation on Tunable Extracellular Matrix Microenvironment

Nanoscale TiO2 nanotubes govern the biological behavior of human glioma and osteosarcoma cells

Enantioselective Baeyer–Villiger Oxidation of Bicyclo[3.2.0]hept-2-en-6-One with Fungi: Optimization of Biotransformation and Use of TiO2 as Support of Cell Growth

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