Titanium Culture Vessel Capable of Controlling Culture Temperature for Evaluation of Cell Thermotolerance

Abstract

Surgery, radiation therapy, and chemical therapy have been reported as the main treatments for cancer, which is one of the deadliest reported diseases. However, because of the high invasiveness of patients, cancer hyperthermia has been studied as a non-invasive treatment. Hyperthermia uses a difference in thermal tolerance between normal and cancer cells and provides an affected part thermal stimulation to kill cancer cells selectively. To develop effective conditions for hyperthermia, an in vitro study to evaluate the thermal tolerance is required. However, because the existing cell culture vessels cannot control the culture temperature, genuine thermal tolerance of cells cannot be investigated appropriately. To reveal the critical temperature proper for hyperthermia, we developed a culture device controlling culture temperature. With the developed device, culture temperature was regulated considerably more quickly than the conventional method with the existing vessels, which enabled us to study the thermal tolerance of cells appropriately. For the control of culture temperature, the device has a titanium culture substrate, where a Peltier element is adhered. Because the biocompatibility of the device was confirmed, the difference in thermal tolerance between normal and cancer cells was investigated using the developed device as well as normal human dermal fibroblasts and Michigan Cancer Foundation-7 as model cell species. Therefore, it was confirmed that cancer cells were more sensitive to thermal stimulation than normal cells, which was qualitatively consistent with previous studies. Thus, our developed device can be used to investigate the thermal tolerance of each cell species, which will contribute to the development of cancer hyperthermia.

The impact of acute and chronic aerobic and resistance exercise on stem cell mobilization: A review of effects in healthy and diseased individuals across different age groups

“…A multitude of studies conducted in the field of biological and medical research have yielded significant results, expanding our understanding of numerous facets of these disciplines [ [104] , [105] , [106] , [107] , [108] ]. One important area that has been extensively explored is the regulation of RNA expression [ [109] , [110] , [111] , [112] , [113] ]. Through meticulous investigations, researchers have elucidated the intricate mechanisms governing the control and modulation of RNA expression, unravelling the complex interplay between transcriptional and post-transcriptional processes [ 114 ].…”

Titanium Culture Vessel Presenting Temperature Gradation for the Thermotolerance Estimation of Cells

“…However, in such experiments, the thermal stimulation of cells cannot be immediately and accurately regulated because the temperature the cells are exposed to is indirectly regulated via the incubator atmosphere. Thus, we fabricated a culture system in which the temperature to which cells were exposed could be accurately controlled using a metallic culture vessel and Peltier element to construct a database with precise information on the thermotolerance of each cell type [ 18 , 19 ]. The advantage of a metallic culture vessel, owing to its capability of accurate temperature regulation, has been demonstrated experimentally, resulting in an effective culture system for investigating the thermal tolerance of cells.…”