Tissues from Post-Mortem Donors as Alternative Sources of Stem Cells for Regenerative Medicine

Haring, Gregor; Zupan, Janja

doi:10.1007/5584_2020_492

Cited by 3 publications

(4 citation statements)

References 43 publications

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“…Post mortem tissues represent plentiful alternative sources for such cellular therapies, in particular from young donors without signs of age-associated joint degeneration. Previous studies have shown that primary cells with MSC-like properties can be obtained from various tissues, even several days post mortem [ 15 , 16 , 18 , 22 , 23 , 24 ], and from tissues stored under cryoprotection for years [ 25 ]. However, comprehensive studies that include several tissues from human post mortem donors along with specific times post mortem are scarce.…”

Section: Discussionmentioning

confidence: 99%

“…The tissues harvested from post mortem donors represent an alternative tissue source for isolating MSCs, particularly with the availability and ease of access to large numbers of biopsies [ 15 , 16 ]. Studies have shown that primary cells from various tissues of cadaveric donors can be isolated several hours, or even several days, post mortem [ 15 ]. Reassuringly, studies that have compared the properties of MSCs derived from cadaveric donors with those from living donors have shown comparable results [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…Reassuringly, studies that have compared the properties of MSCs derived from cadaveric donors with those from living donors have shown comparable results [ 17 , 18 ]. However, face-to-face comparisons of the effects of various times post mortem on the properties of primary MSC-like cells derived from different tissues are scarce [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Knee and Peri-Knee Tissues of Post Mortem Donors Are Strategic Sources of Mesenchymal Stem/Stromal Cells for Regenerative Procedures

Haring

Zupan

2022

IJMS

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Tissues of post mortem donors represent valuable alternative sources for the isolation of primary cells with mesenchymal stem/stromal cell (MSC)-like properties. However, the properties of primary cells derived from different tissues and at different post mortem times are poorly recognized. Here, we aim to identify the optimal tissue source between three knee and peri-knee tissues for the isolation of primary cells with MSC-like properties, and to define the influence of the time post mortem on the properties of these cells. We harvested tissues from subchondral bone marrow, synovium and periosteum from 32 donors at various post mortem times. Primary cells were evaluated using detailed in vitro analyses, including colony formation, trilineage differentiation, immunophenotyping and skeletal stem cell marker-gene expression profiling. These data show that the primary cells with MSC-like properties isolated from these three tissues show no differences in their properties, except for higher expression of CD146 in bone-marrow cells. The success rate of the primary cell isolation is dependent on the post mortem time. However, synovium and periosteum cells isolated more than 48 h post mortem show improved osteogenic and chondrogenic potential. This study suggests that knee and peri-knee tissues from donors even 3 days post mortem are strategic sources of MSCs for regenerative procedures.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Knee and Peri-Knee Tissues of Post Mortem Donors Are Strategic Sources of Mesenchymal Stem/Stromal Cells for Regenerative Procedures

Haring

Zupan

2022

IJMS

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“…The scaffold supports the adhesion and proliferation of human adipose-derived stem cells (hADSCs) and prolongs the viability and insulin-producing function of pancreatic islets [ 18 ]. Tissues from postmortem donors were also proposed as valuable alternative sources for the isolation of primary cells with MSC-like properties [ 19 , 20 ]. Haring et al reported the identification of an optimal tissue source among three knee and peri-knee tissues for the isolation of primary cells with MSC-like properties, and described the effect of the postmortem time on the properties of these cells [ 21 ].…”

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confidence: 99%

New Sources, Differentiation, and Therapeutic Uses of Mesenchymal Stem Cells 2.0

Jung

Park

2023

IJMS

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Recovery of proliferative fibroblast cells from refrigerated sheep skin after different postmortem time intervals and their characterization

Singh

2022

Preprint

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Background Postmortem tissues are a potential source of stem/progenitor cells for cellular therapies, preservation of germplasm and revival of endangered and/or dead species by cloning. How long they can be recovered after animal death, however, is not known precisely. The objective of this study was to evaluate the window of postmortem interval (PMI) within which live and proliferative cells can be recovered from refrigerated sheep skin. Ear skin was procured from animals from slaughterhouse and stored at 4°C in the lab. Small explants (2–3 mm2) were then cultured in DMEM media supplemented with 10% FBS, 50 units/mL of penicillin, 50 µg/mL of streptomycin, and 2.5 µg/mL of fungizone after different PMI. Outgrowth of cells around the explants was scored after 10–12 days of culture at 37°C in a CO2 incubator and cells from selected PMI were sub-cultured for 3–5 times and characterized with respect to their growth profiles, genetic stability, cryopreservation ability and gene expression. Results A total of 474 explants adhered to dish surface, of which 369 (77.89%) exhibited outgrowth in various PMI including 34.79% of 65-days postmortem (dpm) interval. We observed recovery of proliferative cells up to a maximum of 65 days of PMI. Percent of explants exhibiting outgrowth as well as relative confluence of outgrowing cells decreased with increasing PMI. Comparative Growth Curves and GFP expression patterns, upon transfection with a GFP plasmid, were not significantly different in 0-dpm and 65-dpm cell populations (p < 0.05). Recovered cells cryopreserved with > 80% post-freezing cell-viability and were passaged up to 35 times in in vitro cultures. The cytogenetic analysis of 65-dpm tissue derived cells exhibited a normal female sheep karyotype without any genetic aberrations. Conclusions These results show that normal proliferative cells can be recovered from sheep skin up to about 2 months postmortem, if tissues are kept refrigerated. To our knowledge this is the first report of recovering proliferative cells from mammalian tissues up to such a long time of > 2 months after death. The discovery has potential applications in preserving veterinary and livestock germplasm after death to revive in future by cloning as well as in cellular therapies in human and veterinary medicine.

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Tissues from Post-Mortem Donors as Alternative Sources of Stem Cells for Regenerative Medicine

Cited by 3 publications

References 43 publications

Knee and Peri-Knee Tissues of Post Mortem Donors Are Strategic Sources of Mesenchymal Stem/Stromal Cells for Regenerative Procedures

Knee and Peri-Knee Tissues of Post Mortem Donors Are Strategic Sources of Mesenchymal Stem/Stromal Cells for Regenerative Procedures

New Sources, Differentiation, and Therapeutic Uses of Mesenchymal Stem Cells 2.0

Recovery of proliferative fibroblast cells from refrigerated sheep skin after different postmortem time intervals and their characterization

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