Tissues expression analysis, novel SNPs of the bovine Angptl4 gene and its effects on bovine bioeconomic traits

Ma, Yun; Chen, Ningbo; Li, Rongrong; Yang, Ting; Xu, Yongjie; Li, Fen; Gao, Hanting; Zheng, Xianrui; Li, Shuyuan; Zhang, Huali; Huang, Yingwei; Bai, Fang; Wang, Junfang; Li, Yafang; Wang, Xinzhuang; Li, Junya

doi:10.1016/j.livsci.2012.06.027

Cited by 4 publications

(1 citation statement)

References 30 publications

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“…In bovine, 533 SNPs of TNF were found in the dbSNP database, but the validation status for most of them is largely unknown. Although published data on the ANGPTL8 gene in domestic animals remain limited, accumulating evidence indicates that ANGPTL family members are significantly associated with bovine growth traits (Chen et al., ; Li et al., ; Ma et al., ). Noticeably, these studies demonstrated a genetic link between these three important adipokine genes and the regulation of lipid and energy metabolisms.…”

Section: Introductionmentioning

confidence: 99%

Tissue expression and variation analysis of three bovine adipokine genes revealed their effect on growth traits in native Chinese cattle

Chen

Han

et al. 2018

Reprod Domestic Animals

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Adipokines play a crucial role in the regulation of energy homeostasis; however, little is known about genetic alterations in this family that may contribute to economic traits in cattle. Therefore, this study conducts transcript profiles, variations and association studies of three major adipokines, leptin (LEP), tumour necrosis factor (TNF) and angiopoietin-like protein 8 (ANGPTL8), to evaluate their effects on native Chinese cattle. Using quantitative real-time PCR, the study revealed that the bovine LEP was expressed primarily in the back and visceral fat, while TNF was predominantly expressed in spleen and ANGPTL8 was mainly expressed in back fat and liver. Five single nucleotide polymorphisms (SNPs) including two missense SNPs (SNP1: g.12254T>C and SNP2: g.14177C>T) in LEP, a synonymous SNP (SNP3: g.2130A>G) in TNF and two SNPs (SNP4: g.629G>A and SNP5: g.884T>C) in the 5'UTR of ANGPTL8 were identified and genotyped in 537 individuals from six Chinese cattle breeds. Bioinformatics analysis revealed that SNP1 might disrupt the efficient binding of LEP to its receptor, SNP3 might affect translation efficiency of TNF, and SNP4 and SNP5 were likely to affect stability, splicing and nuclear export of ANGPTL8 mRNA. Consistently, association studies indicated that three SNPs (SNP1, SNP3 and SNP5) were significantly associated with body weight, heart girth, average daily gain, hip width and body length in 100 Nanyang cattle (p < 0.05). Overall, our results support the view that LEP, TNF and ANGPTL8 could be used as biomarkers to improve the growth performance in Chinese cattle selection programmes.

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Section: Introductionmentioning

confidence: 99%

Tissue expression and variation analysis of three bovine adipokine genes revealed their effect on growth traits in native Chinese cattle

Chen

Han

et al. 2018

Reprod Domestic Animals

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LXRα gene expression, genetic variation and association analysis between novel SNPs and growth traits in Chinese native cattle

Chen

et al. 2013

J Appl Genetics

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Liver X receptor α (LXRα) has emerged as an important regulator of lipid and energy metabolism. In this study, to better understand the effects of LXRα gene on growth traits in cattle, the mRNA tissue expression patterns and the polymorphisms of some exons of LXRα were revealed. The expression profile of the bovine LXRα gene was detected by quantitative real-time polymerase chain reaction (qRT-PCR) in 11 different Jiaxian cattle tissues and was found mainly expressed in spleen, liver, fat tissue, kidney, muscle, and lung. Meanwhile, it showed that four single nucleotide polymorphisms (SNPs), named g.1028 T>C, g.1514 T>C, g.2929G>A, and g.3493 T>C, were detected and 12 different haplotypes were constructed. Haplotype with CCGT was dominant with frequency of 40.8 %. There was a strong link between g.1028 T>C and g.1514 T>C (r (2) = 0.374). Association analysis of SNPs with growth- and body-related traits was carried out in 445 Chinese native cattle. The results displayed that the heterozygous genotypes of g.1028 T>C and g.1514 T>C showed a molecular heterosis on four performance traits related to body size: height at withers, body length, hipbone width, and hip width (P < 0.05). The multiple effects of four sites showed that the height at withers, body length, hipbone width, and hip width of individuals of TC-TC-GG-TT combined genotypes were significantly higher than other genotypes (P < 0.05). The effects of the four loci genotype combination on conformation traits were consistent with the effects of g.1028 T>C and g.1514 T>C loci. The SNPs of g.1028 T>C and g.1514 T>C of the bovine LXRα gene could be potential genetic markers for growth traits in cattle. These results suggest that LXRα gene is expressed in many tissues and may provide primary molecular information for further studies on body size traits in Chinese indigenous cattle.

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GR and Foxa1 promote the transcription of ANGPTL4 in bovine adipocytes

Ning

Wang

et al. 2019

Molecular and Cellular Probes

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Tissues expression analysis, novel SNPs of the bovine Angptl4 gene and its effects on bovine bioeconomic traits

Cited by 4 publications

References 30 publications

Tissue expression and variation analysis of three bovine adipokine genes revealed their effect on growth traits in native Chinese cattle

Tissue expression and variation analysis of three bovine adipokine genes revealed their effect on growth traits in native Chinese cattle

LXRα gene expression, genetic variation and association analysis between novel SNPs and growth traits in Chinese native cattle

GR and Foxa1 promote the transcription of ANGPTL4 in bovine adipocytes

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