Tissue typing amniotic fluid cells: potential use for detection of contaminating maternal cells.

Niazi, Meena; Coleman, D. V.; Mowbray, J. F.; Blunt, S.

doi:10.1136/jmg.16.1.21

Cited by 15 publications

(2 citation statements)

References 5 publications

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“…Different techniques of HLA typing of amniotic fluid cells have been described (Jonasson et al,. 1972;Mattiuz et al, 1974;Niazi et al, 1979;Pollack et al, 1979b). The selection of techniques giving clearcut identification of HLA antigens is the crucial point.…”

Section: Discussionmentioning

confidence: 99%

Prenatal diagnosis of congenital adrenal hyperplasia (21‐OH deficiency type) by HLA typing

et al. 1981

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The close genetic linkage between HLA-B and congential adrenal hyperplasia due to 21-hydroxylase deficiency permits prenatal diagnosis of an affected fetus by HLA typing of amniotic fluid cells in pregnancies at risk. Some families at risk, especially those with an affected girl with ambiguous genitalia, will only plan another pregnancy if a prenatal diagnosis is possible. After HLA typing of the index case, parents and eventually grandparents, the family were informed of the possibility of a prenatal diagnosis. Fibroblast cell lines were initiated from skin biopsies of the index cases and parents and were used as controls in the tests. HLA typing of the fetus was done on amniotic fluid cells grown in vitro using first, a microcytotoxicity test and second quantitative microabsorption test. Ten prenatal diagnoses are reported. In two cases the HLA genotype indicated an affected fetus, examination of the aborted fetuses was in agreement with the diagnosis. In one case an affected male fetus was diagnosed, the pregnancy is in progress. In seven cases an unaffected infant was predicted (four carriers and three homozygous normal infants).

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Section: Discussionmentioning

confidence: 99%

Prenatal diagnosis of congenital adrenal hyperplasia (21‐OH deficiency type) by HLA typing

et al. 1981

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“…Cells from these amniotic fluid specimens were cultured in Carrel flasks for cytogenetic studies. Cultures were sent to St. Mary's Hospital Medical School Department of Pathology, where HLA typing was carried out using a technique developed by Niazi et al (1979). The flasks were trypsinized and the cells were plated out into the wells of a Falcon-Terasaki microtest 0197-385 1/82/0403 19-03 $01 -00 0 1982 by John Wiley & Sons, Ltd.…”

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confidence: 99%

A case of prenatal paternity discrimination

Roberts

Coleman

1982

Prenatal Diagnosis

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Technical and theoretical considerations in the HLA typing of amniotic fluid cells for prenatal diagnosis and paternity testing

Pollack

Braun

et al. 1981

Prenatal Diagnosis

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HLA typing of amniotic fluid cells has been used for the prenatal diagnosis of the HLA linked diseases congenital adrenal hyperplasia (21-OH-deficiency (21-OH-def) type) and complement C4 deficiency and it has also been used for the prenatal determination of paternity. There are, however, technical difficulties in this test associated with the weak expression of some B locus antigens on amniotic fluid cells, and theoretical difficulties related to associations between particular HLA antigens and the 21-OH-def allele. Since certain HLA-B locus antigens are found in significantly increased frequencies among patients with 21-OH-def, there is a relatively high incidence of HLA-B homozygosity among the patients and over 40 per cent of the parents of these patients share one or more HLA-B locus antigens. Results of some prenatal HLA typing tests may thus be difficult to interpret, and supplementary tests should be used whenever possible. HLA typing of amniotic cells is, however, the only available procedure for prenatal diagnosis of C4 deficiency and it is the best available procedure for prenatal determination of paternity. A modification of our original procedure allows HLA typing to be performed with increased numbers of HLA typing sera, and sera with optimum reactivity for amniotic fluid cells have now been selected for the definition of most of the more commonly expressed HLA antigens. Although amniotic fluid cells do not express DR Antigens, amniotic fluid cells can be typed for the HLA-linked marker glyoxalase I (GLO) and this may be the informative for prenatal diagnosis in some cases.

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Tissue typing amniotic fluid cells: potential use for detection of contaminating maternal cells.

Cited by 15 publications

References 5 publications

Prenatal diagnosis of congenital adrenal hyperplasia (21‐OH deficiency type) by HLA typing

Prenatal diagnosis of congenital adrenal hyperplasia (21‐OH deficiency type) by HLA typing

A case of prenatal paternity discrimination

Technical and theoretical considerations in the HLA typing of amniotic fluid cells for prenatal diagnosis and paternity testing

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