Tissue tRNA‐Derived Fragments (tRFs) as Potential Candidates Correlate with Invasiveness in Colorectal Carcinomas

Abstract

<jats:title>Abstract</jats:title> <jats:p>Background Colorectal cancer (CRC), which is one of lethal and invasive cancer with metastasis at the time of diagnosis. Despite, advanced therapy and surgical techniques, Patients with metastases CRC have a poor survival rate. Enhanced predictive biomarkers such as non-coding RNAs (ncRNAs) are needed at the time of diagnosis to better therapies. The tRNA- derived fragments (tRF) are non-protein-coding sequences that probably function as oncogenes or tumor suppressor genes, tRFs produced under physiological or stress conditions from enzymatically cleavage in tRNAs. Methods Documenting the tRNA-derived fragments (tRF) in colorectal cancer and showed that it is significantly down-regulated in colorectal tissue tumors compared to normal tissue samples. Since data distribution was not normal, Mann-Whitney, Kruskal-Wallis, and Spearman correlation coefficient tests were used for analysis. Results Among several tRF, quantitative real-time PCR analyses declared that tRF/miR-1280, tRNA-derived fragment-Asp, tRNA-derived fragment-Val down-regulated in CRC. Notably, its expression was correlated with invasive stage and metastases (p &lt; 0.05). Conclusions This research can elucidate the association between tRFs with the invasion stage and CRC clinical pathology, our results not only expand better understanding about tRNA-derived fragments species in CRC but also highlight the potential usages of tRFs as a promising biomarker in the prognosis of metastatic cancer.</jats:p>