Tissue spray ionization mass spectrometry for rapid recognition of human lung squamous cell carcinoma

Wei, Yiping; Chen, Li–Ru; Zhou, Wei; Chingin, Konstantin; Ouyang, Yongzhong; Zhu, Tenggao; Wen, Hua; Ding, Jianhua; Xu, Jianjun; Chen, Huanwen

doi:10.1038/srep10077

Cited by 48 publications

(49 citation statements)

References 33 publications

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“…In human tissues, a consistent observation of elevated phospholipids has been observed in cancer compared with normal tissues. This includes breast tumors and invasive ductal carcinomas compared with carcinoma in situ, gastric cancers, renal cell carcinomas, human lung squamous cell carcinoma, and colorectal carcinoma . The most frequent observation is of increased PtdCho in tumors, but increases in PtdEtn, PtdIns, and PA have also been observed .…”

Section: Imaging Metabolic Lipid Changesmentioning

confidence: 99%

Imaging of cancer lipid metabolism in response to therapy

et al. 2019

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Lipids represent a diverse array of molecules essential to the cell's structure, defense, energy, and communication. Lipid metabolism can often become dysregulated during tumor development. During cancer therapy, targeted inhibition of cell proliferation can likewise cause widespread and drastic changes in lipid composition. Molecular imaging techniques have been developed to monitor altered lipid profiles as a biomarker for cancer diagnosis and treatment response. For decades, MRS has been the dominant non-invasive technique for studying lipid metabolite levels. Recent insights into the oncogenic transformations driving changes in lipid metabolism haverevealed new mechanisms and signaling molecules that can be exploited using optical imaging, mass spectrometry imaging, and positron emission tomography. These novel imaging modalities have provided researchers with a diverse toolbox to examine changes in lipids in response to a wide array of anticancer strategies including chemotherapy, radiation therapy, signal transduction inhibitors, gene therapy, immunotherapy, or a combination of these strategies. The understanding of lipid metabolism in response to cancer therapy continues to evolve as each therapeutic method emerges, and this review seeks to summarize the current field and areas of unmet needs.

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Section: Imaging Metabolic Lipid Changesmentioning

confidence: 99%

Imaging of cancer lipid metabolism in response to therapy

et al. 2019

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“…To create a high electric field, the sharp tip is needed which could be either natural or cut in a leaf or other part of the plant. Tissues from animal and human also have been used for direct chemical analysis [43][44][45][46].…”

Section: Leaf Spraymentioning

confidence: 99%

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

2016

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Paper spray mass spectrometry (MS) is part of a cohort of ambient ionization or direct analysis methods that seek to analyze complex samples without prior sample preparation. Extraction and electrospray ionization occur directly from the paper substrate upon which a dried matrix spot is stored. Paper spray MS is capable of detecting drugs directly from dried blood, plasma, and urine spots at the low ng/mL to pg/mL levels without sample preparation. No front end separation is performed, so tandem mass spectrometry (MS/MS) or high resolution MS is required. Here, we discuss paper spray methodology, give a comprehensive literature review of the use of paper spray MS for bioanalysis, discuss technological advancements and variations on this technique, and discuss some of its limitations. _________________________________________________________________________________ AbstractPaper spray mass spectrometry (MS) is part of a cohort of ambient ionization or direct analysis methods that seek to analyze complex samples without prior sample preparation. Extraction and electrospray ionization occur directly from the paper substrate upon which a dried matrix spot is stored. Paper spray MS is capable of detecting drugs directly from dried blood, plasma, and urine spots at the low ng/mL to pg/mL levels without sample preparation. No front end separation is performed, so tandem mass spectrometry (MS/MS) or high resolution MS is required. Here, we discuss paper spray methodology, give a comprehensive literature review of the use of paper spray MS for bioanalysis, discuss technological advancements and variations on this technique, and discuss some of its limitations.

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“…Therefore, the signal detected at m/z 756 was attributed to dipalmitoyl phosphatidylcholine sodium salt, a main component of pulmonary surfactant. [29][30][31]…”

Section: Qualitative Analysis Of Sputum By Nd-eesi-msmentioning

confidence: 99%

Direct Analysis of Human Sputum for Differentiating Non-small Cell Lung Cancer by Neutral Desorption Extractive Electrospray Ionization Mass Spectrometry

2018

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Human sputum, a typical highly viscous biosample, was directly characterized at the molecular level using neutral desorption extractive electrospray ionization mass spectrometry (ND-EESI-MS) without multi-step sample pretreatment, in an attempt to provide a method for constructing the pattern recognition of rapid diagnosis of lung cancer. Under the optimal experiment conditions, glucose, amino acids, phosphoric lipids and other typical analytes in the sputum sample could be used to conduct qualitative or quantitative (in arginine) analysis. More interestingly, the full scan mass spectra from 50 patients of non-small cell lung cancer, recording the mass spectral fingerprints of sputum samples, were differentiated from the control group (50 healthy individuals) through principal component analysis (PCA). These findings suggest that valuable molecular information concealed in human sputum could be easily revealed and applied for conducting qualitative or quantitative analysis by direct ND-EESI-MS analysis.

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Tissue spray ionization mass spectrometry for rapid recognition of human lung squamous cell carcinoma

Cited by 48 publications

References 33 publications

Imaging of cancer lipid metabolism in response to therapy

Imaging of cancer lipid metabolism in response to therapy

Analysis of Biofluids by Paper Spray MS: Advances and Challenges

Direct Analysis of Human Sputum for Differentiating Non-small Cell Lung Cancer by Neutral Desorption Extractive Electrospray Ionization Mass Spectrometry

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