Tissue Spheroids Encaged Into Microscaffolds with Internal Structure to Increase Cell Viability

Dernowsek, Janaina; Rezende, Rodrigo Alvarenga; Passamai, V.E.; Noritomi, Pedro Yoshito; Kemmoku, Daniel Takanori; Nogueira, Júlia A.; Lara, V.F.; Mironov, Vladimir; Silva, J.V.L. da

doi:10.1016/j.procir.2015.11.010

Cited by 5 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pre-processing stage includes the following stages: image acquisition and processing, project design, 3D modeling and/or predictive mathematical methods (BioCAE), selection of the bioink/biomaterial based on the type of 3D bioprinter and on the properties required for the end product [24].…”

Section: Discussionmentioning

confidence: 99%

Piezoelectric 3D bioprinting for ophthalmological applications: process development and viability analysis of the technology

Hoehne¹,

Carlstron²,

Dernorwsek³

et al. 2020

Biomed. Phys. Eng. Express

View full text Add to dashboard Cite

Piezoelectric inkjet 3D bioprinting technology is a viable technique for ophthalmological applications. It provides versatility, high sensibility and accuracy, required in ophthalmological procedures. A process flow for biofabrication was described in detail and validated, using piezoelectric inkjet technology, for ophthalmological applications, in vitro and in situ, based on complex images. Ophthalmological problems were documented by diagnostic examinations and were fed to the flow as complex images. The Concept Mapping methodology and the Conceptual Design approach were utilized to elaborate the 3D bioprinting process flow. It was developed a bioink with corneal epithelial cells. To simulate an in situ bioprinting process, eyes of pigs were selected as the substrate to print the cells. Print scripts used the digitally treated images. In order to print on predefined locations, alignment devices and sample holders were built. The proposed process flow has shown to be a potential tool for the biofabrication of ophthalmological solutions.

show abstract

Section: Discussionmentioning

confidence: 99%

Piezoelectric 3D bioprinting for ophthalmological applications: process development and viability analysis of the technology

Hoehne¹,

Carlstron²,

Dernorwsek³

et al. 2020

Biomed. Phys. Eng. Express

View full text Add to dashboard Cite

show abstract

“…Modelling and simulation of diffusion process in tissue spheroids CFD [14] Agent-based virtual tissue simulations CC3D [17] Cell compressibility, motility and contact inhibition on the growth of tumor cell clusters CC3D [18] Multiscale modeling of the early CD8 T cell immune response in lymph nodes CC3D [19] Multi-scale knowledge on cardiac development CC3D [20] The cell behavior ontology CC3D [21] Cell differentiation in the transition to multicellularity CC3D [22] Dynamics of cell aggregates fusion CC3D [36] A multi-cell model of tumor evolution CC3D [37] Virtual tissues MAS [38] Disruption of blood vessel development CC3D [39] Tumor growth and angiogenesis CC3D [40] Agent-oriented in silico liver (ILS) MAS [41] Model of thrombus development MAS (TS) [42] Three-dimensional multi-scale tumor model MAS [43] A multi-scale model of dendritic cell education and trafficking in the lung CM [44] Multi-scale model of follicular development CM [45] Multi-scale in silico leukocytes model MAS [46] Multi-scale model of organogenesis CM [47] Limitations of spheroids under inappropriate conditions FE [48] Finite lower levels occur at multiple time scales influencing the behavior of the organ. Multi-scale models are usually based on the continuum modeling and/or MAS approaches which can be decomposed into N single-scale mathematical models and several physical processes.…”

Section: Strategy Referencesmentioning

confidence: 99%

“…Multi-scale models are usually based on the continuum modeling and/or MAS approaches which can be decomposed into N single-scale mathematical models and several physical processes. Various works have already been done which apply many multi-scale methods in several biological systems [14,[17][18][19][20][21][22][36][37][38][39][40][41][42][43][44][45][46][47][48][49]: a good review is proposed in Table 1.…”

Section: Strategy Referencesmentioning

confidence: 99%

BioCAE: A New Strategy of Complex Biological Systems for Biofabrication of Tissues and Organs

Dernowsek¹,

Ra²,

Silva³

2017

J Tissue Sci Eng

View full text Add to dashboard Cite

Next sessions we expose a new approach, based on Information *Corresponding author: Janaina de Andrea Dernowsek, Center for information technology Renato Archer (CTI), 3D Technologies Research Group (NT3D), Campinas, Brazil, AbstractBiofabrication as an interdisciplinary area is fostering new knowledge and integration of areas like nanotechnology, chemistry, biology, physics, materials science, control systems, among many others, necessary to accomplish the challenge of bioengineering functional complex tissues. The emergence of integrated platforms and systems biology to understand complex biological systems in multiscale levels will enable the prediction and creation of biofabricated biological structures. This systematic analysis (meta-analysis) or integrated platforms for estimating biological process have been named as BioCAE, which will become the key for important steps of the biofabrication processes. Biological Computational Aided Engineering (BioCAE) is a new computational approach to understanding and bioengineer complex tissues (biofabrication) using a combination of different methods as multiscale modelling, computer simulations, data mining and systems biology. In addition, multi-agent systems (MAS), which are composed of different interacting computing entities called agents, also provide an interesting way to design and implement simulations of biological systems, integrating them with all steps of the BioCAE. MAS as a part of computational science have become a growing area to manipulate and solve complex problems. This paper presents an approach that will allow predicting the development and behavior of different biological processes such as molecular networks, gene interactions, cells, tissues and organs due to its flexibility, beyond to provide a new outlook in the biofabrication of tissues and organs.

show abstract

Basic Science Perspective on Engineering and Modeling the Large Airways

Gautam

Harriott

Caceres

et al. 2023

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Tissue Spheroids Encaged Into Microscaffolds with Internal Structure to Increase Cell Viability

Cited by 5 publications

References 8 publications

Piezoelectric 3D bioprinting for ophthalmological applications: process development and viability analysis of the technology

Piezoelectric 3D bioprinting for ophthalmological applications: process development and viability analysis of the technology

BioCAE: A New Strategy of Complex Biological Systems for Biofabrication of Tissues and Organs

Basic Science Perspective on Engineering and Modeling the Large Airways

Contact Info

Product

Resources

About