Tissue specificity of 3′-untranslated sequence of myosin light chain gene: Unexpected interspecies homology with repetitive DNA

Saidapet, Chandrika; Khandekar, Pramod; Mendola, Charmaine E.; Siddiqui, M.A.Q.

doi:10.1016/0003-9861(84)90480-6

Cited by 18 publications

(6 citation statements)

References 28 publications

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“…Ventricular MLC2 gene was the experimental marker of choice for elucidation of tissue-specific regulation of gene activity, as it exhibits strict cardiac tissue specificity (2,50,54). Although previous studies have characterized cardiogenesis by immunochemical and/or molecular approaches (6,14,17,23,37,49,58,59), little information is available on molecular events in early stages of development during which the committed precardiac mesoderm enters cardiogenic differentiation.…”

Section: Resultsmentioning

confidence: 99%

Differential expression of the myocyte enhancer factor 2 family of transcription factors in development: the cardiac factor BBF-1 is an early marker for cardiogenesis.

Goswami¹,

Qasba²,

Ghatpande³

et al. 1994

Mol. Cell. Biol.

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In the present study, we have used single chicken blastoderms of defined early developmental stages, beginning with the prestreak stage, stage 1 (V. Hamburger and H. L. Hamilton, J. Morphol. 88:49-92, 1951), to analyze the onset of cardiac myogenesis by monitoring the appearance of selected cardiac muscle tissue-specific gene transcripts and the functional expression of the myocyte enhancer factor 2 (MEF-2) proteins. Using gene-specific oligonucleotide primers in reverse transcriptase PCR assay, we have demonstrated that the cardiac myosin light-chain 2 (MLC2) and alpha-actin gene transcripts appear as early as stage 5, i.e., immediately after the cardiogenic fate assignment at stage 4. Consistent with this observation is the developmental expression pattern of DNA-binding activity of BBF-1, a cardiac muscle-specific member of the MEF-2 protein family, which also begins at stage 5 prior to MEF-2. Differential expression of DNA-binding complexes is also observed with another AT-rich DNA sequence (CArG box) as probe, but the binding pattern with the ubiquitous TATA-binding proteins remains unchanged during the same developmental period. Thus, the cardiogenic commitment and differentiation of the precardiac mesoderm, as exemplified by the appearance of cardiac MEF-2, MLC2, and alpha-actin gene products, occur earlier than previously thought and appear to be closely linked. The onset of skeletal myogenic program follows that of the cardiogenic program with the appearance of skeletal MLC2 at stage 8. We also observed that mRNA for the MEF-2 family of proteins appears as early as stage 2 and that for CMD-1, the chicken counterpart of MyoD, appears at stage 5. The temporal separation of activation of cardiac and skeletal MLC2 genes, which appears immediately after the respective fate assignments, and those of cardiac MEF-2 and CMD-1, which occur before, are consistent with the established appearance of the myogenic programs and with the acquisition pattern of the two tissue-specific morphological characteristics in the early embryo. The preferential appearance of BBF-1 activity in precardiac moesderm, relative to that of MEF-2, indicates that these two protein factors are distinct members of the MEF-2 family and provides a compelling argument in support of the potential role of BBF-1 as a regulator of the cardiogenic cell lineage determination, while cardiac MEF-2 might be involved in maintenance of the cardiac differentiative state.

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Section: Resultsmentioning

confidence: 99%

Differential expression of the myocyte enhancer factor 2 family of transcription factors in development: the cardiac factor BBF-1 is an early marker for cardiogenesis.

Goswami¹,

Qasba²,

Ghatpande³

et al. 1994

Mol. Cell. Biol.

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“…The physiological expression of cardiac myosin light-chain 2 (MLC-2) is restricted to cardiac cells, at least in the late embryonic to adult stages (1,57). However, it is believed that the cardiac MLC isoforms appear in both the cardiac and skeletal tissues during early fetal cell development (71), suggesting that the cardiac MLC-2 gene is capable of expression in early development but is repressed in late embryonic and adult noncardiac muscle tissues.…”

mentioning

confidence: 99%

Tissue-specific transcription of the cardiac myosin light-chain 2 gene is regulated by an upstream repressor element.

Shen¹,

Goswami²,

Mascareno³

et al. 1991

Mol. Cell. Biol.

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Physiological expression of the cardiac muscle myosin light-chain 2 (MLC-2) gene in chickens is restricted to cardiac muscle tissue only, at least during the late embryonic to adult stages of development. The mechanism by which cardiac MLC-2 gene expression is repressed in differentiated noncardiac muscle tissues is unknown. Using sequential 5'-deletion mutants of the cardiac MLC-2 promoter introduced into primary skeletal muscle cells in culture, we have demonstrated that a 89-bp region, designated the cardiac-specific sequence (CSS), is essential for repression of cardiac MLC-2 expression in skeletal muscle. Removal of the CSS sequence alone allows transcription in skeletal muscle cells without affecting the transcriptional activity of the promoter in cardiac muscle cells. DNase I footprinting and gel shift assays indicate that protein binding to sequences in the CSS domain occurs readily in nuclear extracts obtained from skeletal muscle but not in extracts isolated under identical conditions from cardiac muscle. Thus, it appears that a negative regulatory mechanism accounts for the lack of expression of the cardiac MLC-2 gene in skeletal muscle and that the CSS element and its binding proteins are important functional components of the regulatory apparatus which ensures the developmental program for cardiac tissue-specific gene expression.

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“…Cardiac myosin light-chain 2 (MLC-2) is known to be expressed in both cardiac and skeletal muscles in early fetal cells (Saidepet et al 1984). The expression of MLC-2 undergoes transcriptional repression in adult non-cardiac muscle tissues.…”

Section: Discussionmentioning

confidence: 99%

Expression of Myotilin During Chicken Development

Dube

Wang

Pellenz

et al. 2014

The Anatomical Record

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Several missense mutations in the Z‐band protein, myotilin, have been implicated in human muscle diseases such as myofibrillar myopathy, spheroid body myopathy, and distal myopathy. Recently, we have reported the cloning of chicken myotilin cDNA. In this study, we have investigated the expression of myotilin in cross‐striated muscles from developing chicken by qRT‐PCR and in situ hybridizations. In situ hybridization of embryonic stages shows myotilin gene expression in heart, somites, neural tissue, eyes and otocysts. RT‐PCR and qRT‐PCR data, together with in situ hybridization results point to a biphasic transcriptional pattern for MYOT gene during early heart development with maximum expression level in the adult. In skeletal muscle, the expression level starts decreasing after embryonic day 20 and declines in the adult skeletal muscles. Western blot assays of myotilin in adult skeletal muscle reveal a decrease in myotilin protein compared with levels in embryonic skeletal muscle. Our results suggest that MYOT gene may undergo transcriptional activation and repression that varies between tissues in developing chicken. We believe this is the first report of the developmental regulation on myotilin expression in non‐mammalian species. Anat Rec, 297:1596–1603, 2014. © 2014 Wiley Periodicals, Inc.

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Tissue specificity of 3′-untranslated sequence of myosin light chain gene: Unexpected interspecies homology with repetitive DNA

Cited by 18 publications

References 28 publications

Differential expression of the myocyte enhancer factor 2 family of transcription factors in development: the cardiac factor BBF-1 is an early marker for cardiogenesis.

Differential expression of the myocyte enhancer factor 2 family of transcription factors in development: the cardiac factor BBF-1 is an early marker for cardiogenesis.

Tissue-specific transcription of the cardiac myosin light-chain 2 gene is regulated by an upstream repressor element.

Expression of Myotilin During Chicken Development

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