Tissue specificity and responses to abiotic stresses and hormones of PIN genes in rice

Xu, Huawei; Zhang, Yongfeng; Yang, Xiaoyi; Hou, Dan

doi:10.1007/s11756-022-01031-9

Cited by 8 publications

(15 citation statements)

References 82 publications

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“…In wheat, Kumar et al (2021) found multiple PIN genes to be upregulated during drought and heat stress. Xu et al (2022) found induction of OsPIN5B and OsPIN9 under various abiotic stress conditions. Our study revealed the upregulation of PIN5C and PIN9 during salt stress and PIN2, PIN5C, and PIN9 during drought stress.…”

Section: Discussionmentioning

confidence: 87%

Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice

et al. 2022

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The auxin efflux transporter proteins called PINs ferry auxin from its source to sinks in particular directions depending on their polar localizations in the plasma membrane, thus facilitating the development of the entire plant architecture. The rice genome has 12 PIN genes distributed over eight chromosomes. To study their roles in plant development, abiotic stress responsiveness, and shaping an auxin-dependent root architecture, a genome-wide analysis was carried out. Based on phylogeny, cellular localization, and hydrophilic loop domain size, the PINs were categorized into canonical and noncanonical PINs. PINs were found expressed in all of the organs of plants that emphasized their indispensable role throughout the plant’s life cycle. We discovered that PIN5C and PIN9 were upregulated during salt and drought stress. We also found that regardless of its cellular level, auxin functioned as a molecular switch to turn on auxin biosynthesis genes. On the contrary, although PIN expression was upregulated upon initial treatment with auxin, prolonged auxin treatment not only led to their downregulation but also led to the development of auxin-dependent altered root formation in rice. Our study paves the way for developing stress-tolerant rice and plants with a desirable root architecture by genetic engineering.

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Section: Discussionmentioning

confidence: 87%

Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice

et al. 2022

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“…Previously, we have reported that OsPIN9 is suppressed by chilling treatment, while induced by salt and drought treatments as well as ABA, gibberellin (GA), methyl jasmonate (MeJA), and salicylic acid (SA) treatments ( Xu et al, 2022 ). Additionally, cis -element analysis of OsPIN9 promoter also showed that the OsPIN9 promoter contains many cis -elements that are predicted to be responsible to auxin, ABA, GA, MeJA, and SA ( Xu et al, 2022 ), implying that OsPIN9 might have a role under diverse abiotic stresses and be implicated in the interconnection of diverse phytohormones by influencing auxin homeostasis.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-mediated mutation in auxin efflux carrier OsPIN9 confers chilling tolerance by modulating reactive oxygen species homeostasis in rice

Yang

Zhang

et al. 2022

Front. Plant Sci.

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Phytohormone auxin plays a vital role in plant development and responses to environmental stresses. The spatial and temporal distribution of auxin mainly relies on the polar distribution of the PIN-FORMED (PIN) auxin efflux carriers. In this study, we dissected the functions of OsPIN9, a monocot-specific auxin efflux carrier gene, in modulating chilling tolerance in rice. The results showed that OsPIN9 expression was dramatically and rapidly suppressed by chilling stress (4°C) in rice seedlings. The homozygous ospin9 mutants were generated by CRISPR/Cas9 technology and employed for further research. ospin9 mutant roots and shoots were less sensitive to 1-naphthaleneacetic acid (NAA) and N-1-naphthylphthalamic acid (NPA), indicating the disturbance of auxin homeostasis in the ospin9 mutants. The chilling tolerance assay showed that ospin9 mutants were more tolerant to chilling stress than wild-type (WT) plants, as evidenced by increased survival rate, decreased membrane permeability, and reduced lipid peroxidation. However, the expression of well-known C-REPEAT BINDING FACTOR (CBF)/DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 1 (DREB)-dependent transcriptional regulatory pathway and Ca2+ signaling genes was significantly induced only under normal conditions, implying that defense responses in ospin9 mutants have probably been triggered in advance under normal conditions. Histochemical staining of reactive oxygen species (ROS) by 3′3-diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) showed that ospin9 mutants accumulated more ROS than WT at the early stage of chilling stress, while less ROS was observed at the later stage of chilling treatment in ospin9 mutants. Consistently, antioxidant enzyme activity, including catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), improved significantly during the early chilling treatments, while was kept similar to WT at the later stage of chilling treatment, implying that the enhanced chilling tolerance of ospin9 mutants is mainly attributed to the earlier induction of ROS and the improved ROS scavenging ability at the subsequent stages of chilling treatment. In summary, our results strongly suggest that the OsPIN9 gene regulates chilling tolerance by modulating ROS homeostasis in rice.

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“…qRT-PCR was performed according to our previous report [ 18 ]. In brief, total RNA was extracted from the samples of WT and ospin1b mutants using RNAiso Plus (Takara Bio Inc., Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…PIN1 is the first reported auxin efflux carrier that functions in shoot-basipetal auxin transport in Arabidopsis, and pin1 mutants show a classical PIN-like inflorescence [ 15 , 16 ]. Thereafter, the PIN gene family has been extensively investigated in rice ( Oryza sativa L.) [ 17 , 18 , 19 ], maize ( Zea mays L.) [ 20 , 21 , 22 ], sorghum ( Sorghum bicolor ) [ 23 ], soybean ( Glycine max L.) [ 24 , 25 ], cotton ( Gossypium hirsutum ) [ 26 , 27 ], potato ( Solanum tuberosum ) [ 28 ], Chinese cabbage ( Brassica rapa L. ssp. pekinensis) [ 29 ], tomato ( Solanum lycopersicum ) [ 30 ], Medicago truncatula [ 31 ], Populus [ 32 , 33 ], switchgrass ( Panicum virgatum L.) [ 34 ], olive ( Olea europaea ) [ 35 ], pear ( Pyrus pyrifolia ) [ 36 ] and coffee ( Coffea arabica ) [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

Mutation of OsPIN1b by CRISPR/Cas9 Reveals a Role for Auxin Transport in Modulating Rice Architecture and Root Gravitropism

Ouyang

Yang

Zhang

et al. 2022

IJMS

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The distribution and content of auxin within plant tissues affect a variety of important growth and developmental processes. Polar auxin transport (PAT), mainly mediated by auxin influx and efflux transporters, plays a vital role in determining auxin maxima and gradients in plants. The auxin efflux carrier PIN-FORMED (PIN) family is one of the major protein families involved in PAT. Rice (Oryza sativa L.) genome possesses 12 OsPIN genes. However, the detailed functions of OsPIN genes involved in regulating the rice architecture and gravity response are less well understood. In the present study, OsPIN1b was disrupted by CRISPR/Cas9 technology, and its roles in modulating rice architecture and root gravitropism were investigated. Tissue-specific analysis showed that OsPIN1b was mainly expressed in roots, stems and sheaths at the seedling stage, and the transcript abundance was progressively decreased during the seedling stages. Expression of OsPIN1b could be quickly and greatly induced by NAA, indicating that OsPIN1b played a vital role in PAT. IAA homeostasis was disturbed in ospin1b mutants, as evidenced by the changed sensitivity of shoot and root to NAA and NPA treatment, respectively. Mutation of OsPIN1b resulted in pleiotropic phenotypes, including decreased growth of shoots and primary roots, reduced adventitious root number in rice seedlings, as well as shorter and narrower leaves, increased leaf angle, more tiller number and decreased plant height and panicle length at the late developmental stage. Moreover, ospin1b mutants displayed a curly root phenotype cultured with tap water regardless of lighting conditions, while nutrient solution culture could partially rescue the curly root phenotype in light and almost completely abolish this phenotype in darkness, indicating the involvement of the integration of light and nutrient signals in root gravitropism regulation. Additionally, amyloplast sedimentation was impaired in the peripheral tiers of the ospin1b root cap columella cell, while it was not the main contributor to the abnormal root gravitropism. These data suggest that OsPIN1b not only plays a vital role in regulating rice architecture but also functions in regulating root gravitropism by the integration of light and nutrient signals.

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Tissue specificity and responses to abiotic stresses and hormones of PIN genes in rice

Cited by 8 publications

References 82 publications

Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice

Characterization and expression profiling of PIN auxin efflux transporters reveal their role in developmental and abiotic stress conditions in rice

CRISPR/Cas9-mediated mutation in auxin efflux carrier OsPIN9 confers chilling tolerance by modulating reactive oxygen species homeostasis in rice

Mutation of OsPIN1b by CRISPR/Cas9 Reveals a Role for Auxin Transport in Modulating Rice Architecture and Root Gravitropism

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