Tissue-specific regulation of rice molybdenum cofactor sulfurase gene in response to salt stress and ABA

Huang, None Ping-Min; Chen, Jiayi; Wang, Shu‐Jen

doi:10.1007/s11738-008-0264-1

Cited by 13 publications

(8 citation statements)

References 25 publications

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“…Physico-chemical analysis showed that the predicted length, molecular weight, and pI values of HvMCSU are 831 amino acids, 92.21 kDa, and 6.84, respectively (Table 1). These findings were similar to those obtained from Arabidopsis, tomato, and rice orthologs (Xiong et al, 2001;Sagi et al, 2002;Huang et al, 2009). MOSC domain could be an essential component in the formation of diverse metal-sulfur clusters containing conserved cysteine residues (Anantharaman and Aravind, 2002).…”

Section: Primary Sequence Analysessupporting

confidence: 88%

“…Genes for ma-1 (GenBank AF162681; (Amrani et al, 2000)) and for similar loci in Aspergillus nidulans (hxB, GenBank AF128114; (Amrani et al, 2000)), cattle (MCSU, GenBank AB036422; (Watanabe et al, 2000)), humans (hmcs, AA689476; (Amrani et al, 2000); GenBank XP 008726; (Ichida et al, 2001)), and Arabidopsis (aba3, GenBank AF325457 (Seo et al, 2000) and AY034895 (Xiong et al, 2001)) were identified. Recently, Huang et al (2009) identified the rice MCSU cDNA (OsMCSU), which is the first MCSU gene cloned in monocot species. According to the functional domain analysis of the predicted amino acid sequence, the OsMCSU protein contains a Nifs domain at its N-terminus and a MOSC domain at the C-terminus.…”

Section: Introductionmentioning

confidence: 99%

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Barley molybdenum cofactor sulfurase (MCSU): sequencing, modeling, and its comparison to other higher plants

Filiz¹,

Distelfeld²,

Fahima³

et al. 2015

Turk J Agric For

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Molybdenum cofactor sulfurases (MCSUs) are important enzymes for plant development and response to environmental queues, including processes such as nitrogen metabolism and regulation of the abscisic acid levels in plant tissues. We cloned and sequenced MCSU gene from barley and performed in silico comparison with rice, tomato, and Arabidopsis. Physico-chemical properties and subcellular predictions were found to be similar in different plant species. All MCSUs had three critical domains: aminotransferase class-V (Pfam: PF00266), MOSC N-terminal beta barrel (Pfam: PF03476), and MOSC (Pfam: PF03473). Secondary structure analysis revealed that random coils were the most abundant, followed by α-helices and extended strands. Predicted binding sites of MCSUs were different in barley and Arabidopsis, whereas rice and tomato showed the same pattern. A conserved triple-cysteine motif was detected in all MCSUs with cys438-cys440-cys445, cys431-cys433-cys438, cys428-cys430-cys435, and cys425-cys427-cys432 in barley, rice, Arabidopsis, and tomato, respectively. Furthermore, a 3D structure analysis indicated that structural divergences were present in all MCSUs, even in the core domain structure. Phylogenetic analysis of MCSUs revealed that monocot-dicot divergence was clearly observed with high bootstrap values. The results of this study will contribute to the understanding of MCSU genes and proteins in plants. The data of this study will also constitute a scientific basis for wet-lab and in silico studies of MCSUs.

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Section: Primary Sequence Analysessupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Barley molybdenum cofactor sulfurase (MCSU): sequencing, modeling, and its comparison to other higher plants

Filiz¹,

Distelfeld²,

Fahima³

et al. 2015

Turk J Agric For

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“…This indicates again that dehydrin proteins have no significant effect on the cold tolerance of cauliflower microshoots since molybdenum was confirmed earlier in this thesis to significantly improve the cold tolerance of the microshoots (Rihan et al, 2014). Molybdenum has an essential role on the synthesis of ABA (Huang et al, 2009) and ABA was confirmed to have positive effects on the accumulation of dehydrin in cauliflower microshoots. Molybdenum has a negative effect on the accumulation of these proteins.…”

Section: Discussionsupporting

confidence: 59%

An analysis of the development of cauliflower seed as a model to improve the molecular mechanism of abiotic stress tolerance in cauliflower artificial seeds

Rihan

Al-Issawi

Fuller

2017

Plant Physiology and Biochemistry

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The development stages of conventional cauliflower seeds were studied and the accumulation of dehydrin proteins through the maturation stages was investigated with the aim of identifying methods to improve the viability of artificial seeds of cauliflower. While carbohydrate, ash and lipids increased throughout the development of cauliflower traditional seeds, proteins increased with the development of seed and reached the maximum level after 75 days of pollination, however, the level of protein started to decrease after that. A significant increase in the accumulation of small size dehydrin proteins (12, 17, 26 KDa) was observed during the development of cauliflower seeds. Several experiments were conducted in order to increase the accumulation of important dehydrin proteins in cauliflower microshoots (artificial seeds). Mannitol and ABA (Absisic acid) increased the accumulation of dehydrins in cauliflower microshoots while cold acclimation did not have a significant impact on the accumulation of these proteins. Molybdenum treatments had a negative impact on dehydrin accumulation. Dehydrins have an important role in the drought tolerance of seeds and, therefore, the current research helps to improve the accumulation of these proteins in cauliflower artificial seeds. This in turns improves the quality of these artificial seeds. The current results suggest that dehydrins do not play an important role in cold tolerance of cauliflower artificial seeds. This study could have an important role in improving the understanding of the molecular mechanism of abiotic stress tolerance in plants.

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“…In this research, parent gene of circRNA Chr2:226834|229,565, annotated as type II inositol 1,4,5-trisphosphate 5-phosphatase FRA3-like, has been reported to plays an essential role in the secondary wall synthesis in fiber cells and xylem vessels [49]. Another gene, annotated as molybdenum cofactor sulfurase (parent gene of circRNA Chr6:9785683|9,788,312), were found to be an important factor in regulating the ABA levels in plant tissues in response to drought, salt, or ABA treatment [50–52].…”

Section: Resultsmentioning

confidence: 99%

Identification of cucumber circular RNAs responsive to salt stress

et al. 2019

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Background Circular RNAs (circRNAs) are 3′-5′ head-to-tail covalently closed non-coding RNA that have been proved to play essential roles in many cellular and developmental processes. However, no information relate to cucumber circRNAs is available currently, especially under salt stress condition. Results In this study, we sequenced circRNAs in cucumber and a total of 2787 were identified, with 1934 in root and 44 in leaf being differentially regulated under salt stress. Characteristics analysis of these circRNAs revealed following features: most of them are exon circRNAs (79.51%) and they prefer to arise from middle exon(s) of parent genes (2035/2516); moreover, most of circularization events (88.3%) use non-canonical-GT/AG splicing signals; last but not least, pairing-driven circularization is not the major way to generate cucumber circRNAs since very few circRNAs (18) contain sufficient flanking complementary sequences. Annotation and enrichment analysis of both parental genes and target mRNAs were launched to uncover the functions of differentially expressed circRNAs induced by salt stress. The results showed that circRNAs may be paly roles in salt stress response by mediating transcription, signal transcription, cell cycle, metabolism adaptation, and ion homeostasis related pathways. Moreover, circRNAs may function to regulate proline metabolisms through regulating associated biosynthesis and degradation genes. Conclusions The present study identified large number of cucumber circRNAs and function annotation revealed their possible biological roles in response to salt stress. Our findings will lay a solid foundation for further structure and function studies of cucumber circRNAs. Electronic supplementary material The online version of this article (10.1186/s12870-019-1712-3) contains supplementary material, which is available to authorized users.

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Tissue-specific regulation of rice molybdenum cofactor sulfurase gene in response to salt stress and ABA

Cited by 13 publications

References 25 publications

Barley molybdenum cofactor sulfurase (MCSU): sequencing, modeling, and its comparison to other higher plants

Barley molybdenum cofactor sulfurase (MCSU): sequencing, modeling, and its comparison to other higher plants

An analysis of the development of cauliflower seed as a model to improve the molecular mechanism of abiotic stress tolerance in cauliflower artificial seeds

Identification of cucumber circular RNAs responsive to salt stress

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