Tissue-specific murine neutrophil activation states in health and inflammation

Chadwick, Jeffrey W.; Fine, Noah; Khoury, William; Tasevski, Nikola; Sun, Cong; Boroumand, Parastoo; Klip, Amira; Glogauer, Michael

doi:10.1002/jlb.4ab1020-248rrr

Cited by 13 publications

(12 citation statements)

References 39 publications

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“…Compared with neutrophils in other tissues, neutrophils in the oral cavity expressed more CD11a and CD66a. Ligature-induced periodontitis upregulated the expression of CD11b, implying that neutrophils could be transformed into a pro-inflammatory immunophenotype in the oral cavity of healthy and inflamed mice [129].…”

Section: Neutrophilsmentioning

confidence: 99%

Application of Ligature-Induced Periodontitis in Mice to Explore the Molecular Mechanism of Periodontal Disease

Lin

Niimi

Ohsugi

et al. 2021

IJMS

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Periodontitis is an inflammatory disease characterized by the destruction of the periodontium. In the last decade, a new murine model of periodontitis has been widely used to simulate alveolar bone resorption and periodontal soft tissue destruction by ligation. Typically, 3-0 to 9-0 silks are selected for ligation around the molars in mice, and significant bone loss and inflammatory infiltration are observed within a week. The ligature-maintained period can vary according to specific aims. We reviewed the findings on the interaction of systemic diseases with periodontitis, periodontal tissue destruction, the immunological and bacteriological responses, and new treatments. In these studies, the activation of osteoclasts, upregulation of pro-inflammatory factors, and excessive immune response have been considered as major factors in periodontal disruption. Multiple genes identified in periodontal tissues partly reflect the complexity of the pathogenesis of periodontitis. The effects of novel treatment methods on periodontitis have also been evaluated in a ligature-induced periodontitis model in mice. This model cannot completely represent all aspects of periodontitis in humans but is considered an effective method for the exploration of its mechanisms. Through this review, we aimed to provide evidence and enlightenment for future studies planning to use this model.

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Section: Neutrophilsmentioning

confidence: 99%

Application of Ligature-Induced Periodontitis in Mice to Explore the Molecular Mechanism of Periodontal Disease

Lin

Niimi

Ohsugi

et al. 2021

IJMS

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“…4G). No significant differences were observed in the expression of the activation markers CD206 96–99 and CD62L 100–103 by OVA + and OVA ‐ innate immune cells (Fig. 4H‐K).…”

Section: Resultsmentioning

confidence: 92%

Specific innate immune cells uptake fetal antigen and display homeostatic phenotypes in the maternal circulation

Arenas‐Hernandez

Romero

Gershater

et al. 2021

Journal of Leukocyte Biology

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Pregnancy represents a period when the mother undergoes significant immunological changes to promote tolerance of the fetal semi‐allograft. Such tolerance results from the exposure of the maternal immune system to fetal antigens (Ags), a process that has been widely investigated at the maternal‐fetal interface and in the adjacent draining lymph nodes. However, the peripheral mechanisms of maternal‐fetal crosstalk are poorly understood. Herein, we hypothesized that specific innate immune cells interact with fetal Ags in the maternal circulation. To test this hypothesis, a mouse model was utilized in which transgenic male mice expressing the chicken ovalbumin (OVA) Ag under the beta‐actin promoter were allogeneically mated with wild‐type females to allow for tracking of the fetal Ag. Fetal Ag‐carrying Ly6G+ and F4/80+ cells were identified in the maternal circulation, where they were more abundant in the second half of pregnancy. Such innate immune cells displayed unique phenotypes: while Ly6G+ cells expressed high levels of MHC‐II and CD80 together with low levels of pro‐inflammatory cytokines, F4/80+ cells up‐regulated the expression of CD86 as well as the anti‐inflammatory cytokines IL‐10 and TGF‐β. In vitro studies using allogeneic GFP+ placental particles revealed that maternal peripheral Ly6G+ and F4/80+ cells phagocytose fetal Ags in mid and late murine pregnancy. Importantly, cytotrophoblast‐derived particles were also engulfed in vitro by CD15+ and CD14+ cells from women in the second and third trimester, providing translational evidence that this process also occurs in humans. Collectively, this study demonstrates novel interactions between specific maternal circulating innate immune cells and fetal Ags, thereby shedding light on the systemic mechanisms of maternal‐fetal crosstalk.

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“…9c) as expression levels of murine activation markers CD11b, CD62L and CD63 (ref. 21 ), while again LPS significantly increased CD11b and CD63 on the cell surface of murine neutrophils (Extended Data Fig. 9b).…”

Section: Hnp Pfc Uptake and Impact On Human Neutrophil Functionmentioning

confidence: 79%

Non-invasive mapping of systemic neutrophil dynamics upon cardiovascular injury

et al. 2023

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Neutrophils play a complex role during onset of tissue injury and subsequent resolution and healing. To assess neutrophil dynamics upon cardiovascular injury, here we develop a non-invasive, background-free approach for specific mapping of neutrophil dynamics by whole-body magnetic resonance imaging using targeted multimodal fluorine-loaded nanotracers engineered with binding peptides specifically directed against murine or human neutrophils. Intravenous tracer application before injury allowed non-invasive three-dimensional visualization of neutrophils within their different hematopoietic niches over the entire body and subsequent monitoring of their egress into affected tissues. Stimulated murine and human neutrophils exhibited enhanced labeling due to upregulation of their target receptors, which could be exploited as an in vivo readout for their activation state in both sterile and nonsterile cardiovascular inflammation. This non-invasive approach will allow us to identify hidden origins of bacterial or sterile inflammation in patients and also to unravel cardiovascular disease states on the verge of severe aggravation due to enhanced neutrophil infiltration or activation.

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Tissue-specific murine neutrophil activation states in health and inflammation

Cited by 13 publications

References 39 publications

Application of Ligature-Induced Periodontitis in Mice to Explore the Molecular Mechanism of Periodontal Disease

Application of Ligature-Induced Periodontitis in Mice to Explore the Molecular Mechanism of Periodontal Disease

Specific innate immune cells uptake fetal antigen and display homeostatic phenotypes in the maternal circulation

Non-invasive mapping of systemic neutrophil dynamics upon cardiovascular injury

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