Tissue-specific knockout inDrosophilaneuromuscular system reveals ESCRT’s role in formation of synapse-derived extracellular vesicles
Xinchen Chen,
Sarah Perry,
Bei Wang
et al.
Abstract:Tissue-specific gene knockout by CRISPR/Cas9 is a powerful approach for characterizing gene functions in animal development. However, this approach has been successfully applied in only a small number of Drosophila tissues. The Drosophila motor nervous system is an excellent model system for studying the biology of neuromuscular junction (NMJ). To expand tissue-specific CRISPR to the Drosophila motor system, here we present a CRISPR-mediated tissue-restricted mutagenesis (CRISPR-TRiM) toolkit for knocking out … Show more
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