Tissue-specificNIA1andNIA2expression inArabidopsis thaliana

Olas, Justyna Jadwiga; Wahl, Vanessa

doi:10.1080/15592324.2019.1656035

Cited by 23 publications

(17 citation statements)

References 18 publications

(21 reference statements)

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“…WRKY45 is a TF downstream of ERF054 that has been shown to promote both flowering time and LS (Chen, Xiang, et al, 2017). ERF054 is also predicted to be upstream of genes related to flowering time (AGL2/SEP1, AGL8), nitrogen signaling and metabolism (NLP3, NIA1), and stress and LS signaling (LEA5/SAG21, DTX50, WRKY28) (Figures 9 and 10) (Gu et al, 1998;Mohn et al, 2019;Olas & Wahl, 2019;Pan et al, 2019;Pelaz et al, 2000;Salleh et al, 2012;van Verk et al, 2011;Zhang et al, 2014). ERF054 may be a candidate regulatory gene that links stress, LS, nitrogen metabolism, and flowering time signaling.…”

Section: Grnsmentioning

confidence: 99%

Gene expression changes occurring at bolting time are associated with leaf senescence in Arabidopsis

Brusslan

2020

Plant Direct

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In plants, the vegetative to reproductive phase transition (termed bolting in Arabidopsis) generally precedes age‐dependent leaf senescence (LS). Many studies describe a temporal link between bolting time and LS, as plants that bolt early, senesce early, and plants that bolt late, senesce late. The molecular mechanisms underlying this relationship are unknown and are potentially agriculturally important, as they may allow for the development of crops that can overcome early LS caused by stress‐related early‐phase transition. We hypothesized that leaf gene expression changes occurring in synchrony with bolting were regulating LS. ARABIDOPSIS TRITHORAX (ATX) enzymes are general methyltransferases that regulate the adult vegetative to reproductive phase transition. We generated an atx1, atx3, and atx4 (atx1,3,4) triple T‐DNA insertion mutant that displays both early bolting and early LS. This mutant was used in an RNA‐seq time‐series experiment to identify gene expression changes in rosette leaves that are likely associated with bolting. By comparing the early bolting mutant to vegetative WT plants of the same age, we were able to generate a list of differentially expressed genes (DEGs) that change expression with bolting as the plants age. We trimmed the list by intersection with publicly available WT datasets, which removed genes from our DEG list that were atx1,3,4 specific. The resulting 398 bolting‐associated genes (BAGs) are differentially expressed in a mature rosette leaf at bolting. The BAG list contains many well‐characterized LS regulators (ORE1, WRKY45, NAP, WRKY28), and GO analysis revealed enrichment for LS and LS‐related processes. These bolting‐associated LS regulators may contribute to the temporal coupling of bolting time to LS.

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Section: Grnsmentioning

confidence: 99%

Gene expression changes occurring at bolting time are associated with leaf senescence in Arabidopsis

Brusslan

2020

Plant Direct

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“…An unexpected finding of our study, supported by experimental evidence, is that primary carbohydrate metabolism plays a key role in establishing thermomemory in the SAM. function during the fluctuating conditions, a behaviour similar to that observed for NITRATE REDUCTASE 1 and 2 which complement their expression in the SAM (33). Moreover, cytoplasmic aldolases like FBA6 and FBA8 are essential for gluconeogenesis and glycolysis to generate ATP and building blocks for anabolism (27), thereby allowing the cytosolic glycolytic network to provide metabolic flexibility that facilitates acclimation of plants to environmental stresses.…”

Section: Discussionmentioning

confidence: 72%

Primary carbohydrate metabolism genes participate in heat stress memory at the shoot apical meristem ofArabidopsis thaliana

Olas

Apelt

Annunziata

et al. 2020

Preprint

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Although we have a good understanding of the development of shoot apical meristems (SAM) in higher plants, and the function of the stem cells (SCs) embedded in the SAM, there is surprisingly little known of its molecular responses to abiotic stresses. Here, we show that the SAM of Arabidopsis thaliana senses heat stress (HS) and retains an autonomous molecular memory of a previous non-lethal HS, allowing the SAM to regain growth after exposure to an otherwise lethal HS several days later. Using RNA-seq, we identified genes participating in establishing a SAM-specific HS memory. The genes include HEAT SHOCK TRANSCRIPTION FACTORs (HSFs), of which HSFA2 is essential, but not sufficient, for full HS memory in the SAM, the SC regulators CLAVATA1 (CLV1) and CLV3, and several primary carbohydrate metabolism genes, including FRUCTOSE-BISPHOSPHATE ALDOLASE 6 (FBA6). We found that expression of FBA6 during HS at the SAM complements that of FBA8 in the same organ. Furthermore, we show that sugar availability at the SAM is essential for survival at high-temperature HS. Collectively, plants have evolved a sophisticated protection mechanism to maintain SCs and, hence, their capacity to re-initiate shoot growth after stress release.

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“…NIA1 and NIA2 maintain tissue-specific gene expression profiles, with NIA1 expression generally complementing that of NIA2 in the same organ. NIA1 was predominantly found in leaves, while NIA2 was predominantly found in meristematic tissue (Olas & Wahl, 2019). We analyzed whole Arabidopsis rosettes before bolting, of which developing leaves and apical meristematic tissue comprises only a small amount of total tissue.…”

Section: Resultsmentioning

confidence: 99%

Diurnal Dynamics of the Arabidopsis Rosette Proteome and Phosphoproteome

Uhrig

Eschevarria-Zomeno

Schlaepfer

et al. 2020

Preprint

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Plant growth depends on the diurnal regulation of cellular processes, but it is not well understood if and how transcriptional regulation controls diurnal fluctuations at the protein-level. Here we report a high-resolution Arabidopsis thaliana (Arabidopsis) leaf rosette proteome acquired over a 12 h light : 12 h dark diurnal cycle and the phosphoproteome immediately before and after the light-to-dark and dark-to-light transitions. We quantified nearly 5000 proteins and 800 phosphoproteins, of which 288 fluctuated in their abundance and 226 fluctuated in their phosphorylation status. Of the phosphoproteins, 60% were quantified for changes in protein abundance. This revealed six proteins involved in nitrogen and hormone metabolism that had concurrent changes in both protein abundance and phosphorylation status. The diurnal proteome and phosphoproteome changes involve proteins in key cellular processes, including protein translation, light perception, photosynthesis, metabolism and transport. The phosphoproteome at the light-dark transitions revealed the dynamics at phosphorylation sites in either anticipation of or response to a change in light regime. Phosphorylation site motif analyses implicate casein kinase II and calcium/calmodulin dependent kinases among the primary light-dark transition kinases. The comparative analysis of the diurnal proteome and diurnal and circadian transcriptome established how mRNA and protein accumulation intersect in leaves during the diurnal cycle of the plant.Summary StatementThe manuscript provides quantitative information of diurnal changes in the accumulation and phosphorylation of proteins in Arabidopsis thaliana rosettes grown in a 12 h photoperiod. The highly resolved time-scale of the datasets offer new proteome-level insights for future targeted studies.

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Tissue-specificNIA1andNIA2expression inArabidopsis thaliana

Cited by 23 publications

References 18 publications

Gene expression changes occurring at bolting time are associated with leaf senescence in Arabidopsis

Gene expression changes occurring at bolting time are associated with leaf senescence in Arabidopsis

Primary carbohydrate metabolism genes participate in heat stress memory at the shoot apical meristem ofArabidopsis thaliana

Diurnal Dynamics of the Arabidopsis Rosette Proteome and Phosphoproteome

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