Tissue-specific genome instability in synthetic interspecific hybrids of Pennisetum purpureum (Napier grass) and Pennisetum glaucum (pearl millet) is caused by micronucleation

Reis, Gabriela Barreto dos; Ishii, Takayoshi; Fuchs, Joerg; Houben, Andreas; Davide, Lisete Chamma

doi:10.1007/s10577-016-9521-0

Cited by 10 publications

(4 citation statements)

References 45 publications

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“…capillaris [3]. In addition to mutagenesis, this strategy has been also applied by dos Reis et al in their study of tissue-specific instability in synthetic interspecific Pennisetum hybrids [48]. In the case of Brachypodium, 7% of the micronuclei with one telomeric and one centromeric signal were observed.…”

Section: Resultsmentioning

confidence: 99%

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

2017

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Brachypodium distachyon (Brachypodium) is now intensively utilized as a model grass species in various biological studies. Its favorable cytological features create a unique foundation for a convenient system in mutagenesis, thereby potentially enabling the ‘hot spots’ and ‘cold spots’ of DNA damage in its genome to be analyzed. The aim of this study was to analyze the involvement of 5S rDNA, 25S rDNA, the Arabidopsis-type ()n telomeric sequence and the Brachypodium-originated centromeric BAC clone CB33J12 in the micronuclei formation in Brachypodium root tip cells that were subjected to the chemical clastogenic agent maleic hydrazide (MH). To the best of our knowledge, this is the first use of a multicolor fluorescence in situ hybridization (mFISH) with four different DNA probes being used simultaneously to study plant mutagenesis. A quantitative analysis allowed ten types of micronuclei, which were characterized by the presence or absence of specific FISH signal(s), to be distinguished, thus enabling some specific rules governing the composition of the MH-induced micronuclei with the majority of them originating from the terminal regions of chromosomes, to be identified. The application of rDNA sequences as probes showed that 5S rDNA-bearing chromosomes are involved in micronuclei formation more frequently than the 25S rDNA-bearing chromosomes. These findings demonstrate the promising potential of Brachypodium to be a useful model organism to analyze the effects of various genotoxic agents on the plant nuclear genome stability, especially when the complex FISH-based and chromosome-specific approaches such as chromosome barcoding and chromosome painting will be applied in future studies.

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Section: Resultsmentioning

confidence: 99%

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

2017

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“…), failures in spindle fibers formation and absence of metaphase plate were noted in the induced polyploids, as well as micro nuclei were observed in subsequent generation showing abnormalities in meiotic divisions [72]. Homoploid Pennisetum purpureum (L.) Brown × Pennisetum glaucum (L.) Brown showed alteration in karyotypes, as chromosomal rearrangement and loss of DNA sequences, thus evidencing that interspecific hybrids showing intergenomic conflicts could lose genomic DNA [73], including genes and DNA sequences of the centromere and telomere. Contrary to these findings, induced polysomic tetraploids of Italian or annual ryegrass (Festuca perennis Lam.…”

Section: Stability Of Induced Polyploidymentioning

confidence: 96%

Induced Polyploidy: A Tool for Forage Species Improvement

et al. 2021

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Polyploidy means having more than two basic sets of chromosomes. Polyploid plants may be artificially obtained through chemical, physical and biological (2n gametes) methods. This approach allows an increased gene scope and expression, thus resulting in phenotypic changes such as yield and product quality. Nonetheless, breeding new cultivars through induced polyploidy should overcome deleterious effects that are partly contributed by genome and epigenome instability after polyploidization. Furthermore, shortening the time required from early chromosome set doubling to the final selection of high yielding superior polyploids is a must. Despite these hurdles, plant breeders have successfully obtained polyploid bred-germplasm in broad range of forages after optimizing methods, concentration and time, particularly when using colchicine. These experimental polyploids are a valuable tool for understanding gene expression, which seems to be driven by dosage dependent gene expression, altered gene regulation and epigenetic changes. Isozymes and DNA-based markers facilitated the identification of rare alleles for particular loci when compared with diploids, and also explained their heterozygosity, phenotypic plasticity and adaptability to diverse environments. Experimentally induced polyploid germplasm could enhance fresh herbage yield and quality, e.g., leaf protein content, leaf total soluble solids, water soluble carbohydrates and sucrose content. Offspring of experimentally obtained hybrids should undergo selection for several generations to improve their performance and stability.

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“…However, the gene balance hypothesis raised by Birchler et al (31,32) indicates that genes on chromosomal segments can show dosage, compensation or inverse dosage effects, which obscured the general expectation of dosage effect. Moreover, it is important to note that aneuploidy contributes to phenotypic variations and the rapid morphological divergence of plants in the progeny population derived from new polyploidization (33). Studies have suggested that aneuploidy has a significant impact on phenotypic variability, including sterility (34), flowering time (35), and disease resistance (36), by introducing alterations in gene expression via gene dosage effects/compensation and by break-associated position effects (28).…”

Section: Introductionmentioning

confidence: 99%

Variations in Homoeologous Dosage and Epigenomics Mark the Early Evolution of SyntheticBrassicaTetraploids

Zhang

Yang

Zhang

et al. 2023

Preprint

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Polyploidization is important in plant evolution and is becoming increasingly important in crop breeding and material creation. Studies have provided evidence for structural variations and epigenomic repatterning in synthetic polyploidizations, but the relationships between structural and epigenomic variations, as well as their effects on gene expression and phenotypic variations are unknown. Here, we investigated the genome-wide copy number variations of fragments (CVFs) and genomic methylation dynamics, in the leaf organ as a representative, of progenies from eight generations that derived from the synthetic tetraploidization between Brassica rapa and Brassica oleracea. We found that half or complete deletion/duplication of fragments ranging in size from 400 kb to 65.85 Mb, with a mean size of 5.70 Mb, occurred frequently from the first generation of selfing and thereafter. The genes located in these CVFs expressed at levels expected for a positive dosage effect, as indicated by the positive association between expression and the copy number of these genes. Plants containing these CVFs also showed distinct phenotypic variations. The whole genome methylation level experienced significant fluctuations in different generations and eventually decreased in the latter generations. Moreover, the CVFs did not show methylation changes from other individuals of the same generation, and the local regions with methylation alterations did not affect gene expression. Together, the dosage effect of CVFs on gene expression was likely the causal factor underlying phenotypic variations in synthetic tetraploids. Our findings provide new insights into the early evolution of polyploid genomes and guide the use of synthetic polyploidizations in breeding.

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Tissue-specific genome instability in synthetic interspecific hybrids of Pennisetum purpureum (Napier grass) and Pennisetum glaucum (pearl millet) is caused by micronucleation

Cited by 10 publications

References 45 publications

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

Brachypodium distachyon – A Useful Model in the Qualification of Mutagen-Induced Micronuclei Using Multicolor FISH

Induced Polyploidy: A Tool for Forage Species Improvement

Variations in Homoeologous Dosage and Epigenomics Mark the Early Evolution of SyntheticBrassicaTetraploids

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