Tissue specific gene activities and proteins in the Chironomus salivary gland

Wobus, Ulrich; Panitz, Reinhard; Serfling, Edgar

doi:10.1007/bf00268696

Cited by 29 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BEERMANN (1961) and GROSSBACK (1968) working in Chironomus found strong correlation between certain puffs and cell products. WoBus, PANITZ and SERFLING (1970) and WoBus, SERFLING and PANITZ ( 1971 ), in their intensive analysis of the polypeptide at different stages of development of the salivary gland of Chironomidae, found variations in the polypeptides and also changes in puff activity, however, they could not find any direct correlation between any specific puff with a specific polypeptide.…”

Section: Discussionmentioning

confidence: 96%

DNA and RNA Puffs inRhynchosciara

Guevara

Basile

1973

Caryologia

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 96%

DNA and RNA Puffs inRhynchosciara

Guevara

Basile

1973

Caryologia

View full text Add to dashboard Cite

“…This may be due to a continuation of the translational activity of the messenger for several hours (approximately 4 -6 h) after the deactivation of the ring. The relatively low molecular weight of this polypeptide comes in contrast with the size of BR 2 or the size of the translation products of the BRs in Chironornus larvae (Grossbach, 1968(Grossbach, , 1969Wobus et al, 1970Wobus et al, , 1971Grossbach, 1977;Serfling et aL, 1983). There is a strong evidence however (Scouras & Kastritsis, 1984;Kastritsis et al, 1986), that both BR 1 and BR 2 exist within the limits of duplications and thus they could consist of several small functional units coding for polypeptides o flower molecular weight than the one expected from the size of this giant puff.…”

Section: Discussionmentioning

confidence: 97%

“…In a sense, therefore, the larval profile of the BRs is both different from and similar to the profiles of the intermoult puffs; it is different at the origin but identical at the end of their activity (Scouras & Kastritsis, 1984). Consequently, looking at the larval profiles, it is possible to expect that the rings are involved in the production of a major secretory product, as is the case with the BRs of chironomids (Beerman, 1961;Grossbach, 1968Grossbach, , 1969Grossbach, , 1977Wobus et al, 1970;Wobus et al, 1971;Serfling et al, 1983), and the intermoult puffs of D. melanogaster and other species (Beckendorf & Kafatos, 1976;Korge, 1977a, b;Velissariou & Ashburner, 1980. burner, 1972, 1973, 1974; A s h b u r n e r & Richards, 1976; A s h b u r n e r et al., 1974) and this is certainly the case with the intermoult puffs of D. auraria.…”

Section: Discussionmentioning

confidence: 99%

Possible correlations of polypeptides and Balbiani rings in the salivary glands of Drosophila auraria Peng.

Manousis

Kastritsis

1987

Genetica

View full text Add to dashboard Cite

Salivary glands of various stocks of Drosophila auraria and some of its close relatives were examined with a variety of electrophoretic techniques both from larval and prepupal stages, and after ecdysterone treatments, in an effort to detect possible translation products of the two Balbiani rings (BR1 and BR2) found in the salivary gland chromosomes of these species. Two polypeptides (P2 and P1), with molecular weights of 12,000 and 53,000, respectively, have been detected, the appearance of which coincides with the presence of BR2. The results do not allow the correlation of BR1 action with any specific polypeptide(s).

show abstract

“…In Chironomus, the developmental changes in the amounts of chromosomal and salivary gland proteins have been studied using various techniques (7,24,42) . However, the chromosomal distribution of these proteins was not determined .…”

Section: Discussionmentioning

confidence: 99%

Localization of chromosomal protein HMG-1 in polytene chromosomes of Chironomus thummi.

Kurth¹,

Bustin²

1981

The Journal of Cell Biology

View full text Add to dashboard Cite

The distribution of accessible antigenic sites in the chromosomal protein high mobility group one (HMG-1) in Chironomus thummi polytene chromosomes is visualized by immunofluorescence . The results indicate that (a) HMG-1 is distributed in a distinct banding pattern along the entire length of the chromosomes; (b) the banding pattern obtained with fluorescent antibody does not strictly correspond to that observed by phase-contrast microscopy ; and (c) the amount of HMG-1 increases, and the fluorescent banding pattern changes, during the development of the organism . Our findings suggest that the protein may be involved in the modulation of the structure of selected loci in the chromosome .The nonhistone chromosomal proteins are an integral part of the eucaryotic genome . Although the function of these proteins is not well understood, there is evidence that some proteins belonging to this group are important in maintaining the structure (1, 36) and regulating the function (9, 35, 41) of chromatin and chromosomes. The difficulties in purifying homogeneous molecular species from this group of proteins are a major obstacle in elucidating their function. Chromosomal protein high mobility group one (HMG-1) is one of the few nonhistone proteins that has been purified to homogeneity (11). This protein is ubiquitous in its distribution, as it is found in several eucaryotic kingdoms (15,28,37,38,40). Sequence studies revealed that it is unusually rich in charged amino acid residues, and that the negatively and positively charged residues are clustered on the polypeptide chain (39) . The proteins bind to histones and DNA and can induce changes in the DNA helical structure (17,19,44). Evidence has been presented that this protein is associated with isolated nucleosomes (13) .Antisera, elicited by HMG-1 protein purified from calf thymus, cross react immunologically with HMG-1 derived from several species (33) . The antibodies bind to chromatin (4), allowing these antisera to serve as useful cytological tools to study the cellular function of this protein.In the present study, we investigate the distribution of protein HMG-1 in polytene chromosomes of Chironomus thummi. Polytene chromosomes have the same fundamental chromatin fiber structure as that present in all eucaryotic systems (43) . Their large size offers the advantage of amplification in studying the location of a particular chromosomal protein and in following structural alterations associated with functional changes in the genome. Antisera to RNA polymerase (18), nonhistone proteins (36), histones (5), and fluorescent concanavalin A (22) have already been successfully used for such studies. The present study is the first investigation on the chromosomal localization of a structurally defined nonhistone protein that has been detected in several eucaryotic kingdoms . Affinity-purified antibodies are used to demonstrate that Chironomus contains a protein that is indistinguishable from its homologue purified from calf thymus . Its organization in the chromosome...

show abstract

Tissue specific gene activities and proteins in the Chironomus salivary gland

Cited by 29 publications

References 20 publications

DNA and RNA Puffs inRhynchosciara

DNA and RNA Puffs inRhynchosciara

Possible correlations of polypeptides and Balbiani rings in the salivary glands of Drosophila auraria Peng.

Localization of chromosomal protein HMG-1 in polytene chromosomes of Chironomus thummi.

Contact Info

Product

Resources

About