Tissue-specific extracellular matrix coatings for the promotion of cell proliferation and maintenance of cell phenotype

Zhang, Yuanyuan; He, Yujiang; Bharadwaj, Shantaram; Hammam, Nevin; Carnagey, Kristen M.; Myers, Regina L.; Atala, Anthony; Dyke, Mark Van

doi:10.1016/j.biomaterials.2009.04.005

Cited by 222 publications

(193 citation statements)

References 27 publications

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“…SMC-ECM induced a higher number of adherent ECs and higher proliferation rate than EC-ECM did, which was different from Wicha et al 17 and Zhang et al 18 The reports showed that cells from special tissue obtained optimal growth and phenotype when they were cultured on the ECM from the same origin. 17,18 However, in our study, ECs grown on SMC-ECM showed elongated morphology and secreted much less NO than those on EC-ECM. Most importantly, the Ti CVIs modified by EC-ECM obtained complete in vivo endothelialization, but not by SMC-ECM.…”

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confidence: 57%

“…17 Zhang et al further confirmed the tissue specificity of the ECM by studying the influences of ECM origin on growth of the corresponding cells, and found that the cells of specific origin obtained a higher proliferation and differentiation rate when they were cultured on the matched ECM. 18 Accordingly, we hypothesized that the ECM of endothelial origin would perform better in sustaining ECs growth and functions, thus perform better in accelerating endothelialization of CVIs. To warrant the hypothesis, ECMs deposited in situ by ECs and smooth muscle cells (SMCs) (referred to as EC-EMC, SMC-ECM, respectively) were applied to modify Ti CVIs, and their performances in improving hemocompatibility and cytocompatibility and in accelerating the in vivo endothelialization rate of CVIs were systematically compared.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Tissue Specificity on the Performance of Extracellular Matrix in Improving Endothelialization of Cardiovascular Implants

Yang

Zhu

et al. 2013

Tissue Engineering Part A

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Natural extracellular matrix (ECM) deposited in situ by cultured endothelial cells (ECs) has been proven effective in accelerating endothelialization of titanium (Ti) cardiovascular implants (CVIs) in our previous studies. In this study, the ECM deposited by smooth muscle cells (SMCs) was used in comparison to investigate the effects of tissue specificity of the ECM on the ability to accelerate endothelialization of CVIs. The results demonstrated that the ECM deposited by ECs and SMCs (EC-ECM, SMC-ECM, respectively) differed considerably in components and fibril morphology. Surface modification of Ti CVIs with both types of natural ECM was effective in improving their in vitro hemocompatibility and cytocompatibility simultaneously. However, the endothelialization of ECM-modified Ti CVIs in a canine model demonstrated a high tissue specificity of the ECM. Although the ECM deposited by SMCs (SMC-ECM) induced fewer platelet adhesion and sustained better growth and viability of ECs in vitro, its performance in accelerating in vivo endothelialization of Ti CVIs was extremely poor. In contrast, the ECM deposited by ECs (EC-ECM) led to complete endothelium formation in vivo.

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confidence: 57%

Section: Introductionmentioning

confidence: 99%

Effect of Tissue Specificity on the Performance of Extracellular Matrix in Improving Endothelialization of Cardiovascular Implants

Yang

Zhu

et al. 2013

Tissue Engineering Part A

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“…These biomaterials can be processed in such a way as to retain growth factors, such as basic fibroblast growth factor (FGF-2) and transforming growth factor-beta (TGF-β), [17,18] as well as glycosaminoglycans such as heparin and dermatan sulphate [19,20]. They provide adequate microenvironment for cells that allows attachment, migration, proliferation and differentiation [21][22][23]. These naturally occurring biomaterials have been shown to integrate seamlessly with the host's tissues, induce the deposition of cells and additional extra cellular matrix (ECM), and promote rapid angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

One and four layer acellular bladder matrix for fascial tissue reconstruction

Eberli

Atala

Yoo

2011

J Mater Sci: Mater Med

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To determine whether the use of multiple layers of acellular bladder matrix (ABM) is more suitable for the treatment of abdominal wall hernia than a single layered ABM. The feasibility, biocompatibility and mechanical properties of both materials were assessed and compared. Biocompatibility testing was performed on 4 and 1 layered ABM. The matrices were used to repair an abdominal hernia model in 24 rabbits. The animals were followed for up to 3 months. Immediately after euthanasia, the implant site was inspected and samples were retrieved for histology, scanning electron microscopy and biomechanical studies. Both acellular biomaterials demonstrated excellent biocompatibility. At the time of retrieval, there was no evidence of infection. The matrices demonstrated biomechanical properties comparable to native tissue. Three hernias (25%) were found in the single layer ABM group and only 1 hernia (8%) was found in the 4 layer ABM group. Histologically, the matrix structure was intact and the cell density within the matrices decreased with time. The dominant cell type present within the matrices shifted from lymphocytes to fibroblasts over time. Both ABMs maintained adequate strength over time when used for hernia repair, and there was an extremely low incidence of adhesion formation. The single layer ABM showed enhanced cellular integration, while the 4 layer ABM reduced hernia formation. Either of these matrices may be useful as an off-the-shelf biomaterial for patients requiring fascial repair. TITLE:ONE To determine whether the use of multiple layers of acellular bladder matrix (ABM) is more suitable for the treatment of abdominal wall hernia than a single layered ABM. The feasibility, biocompatibility and mechanical properties of both materials were assessed and compared.Design and Method: Biocompatibility testing was performed on 4 and 1 layered ABM.The matrices were used to repair an abdominal hernia model in 24 rabbits. The animals were followed for up to 3 months. Immediately after euthanasia, the implant site was inspected and samples were retrieved for histology, scanning electron microscopy and biomechanical studies.Results: Both acellular biomaterials demonstrated excellent biocompatibility. At the time of retrieval, there was no evidence of infection. The matrices demonstrated biomechanical properties comparable to native tissue. Three hernias (25%) were found in the single layer ABM group and only 1 hernia (8%) was found in the 4 layer ABM group.Histologically, the matrix structure was intact and the cell density within the matrices decreased with time. The dominant cell type present within the matrices shifted from lymphocytes to fibroblasts over time.

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“…Recent work has described the potential benefits of ECM scaffold materials derived from homologous tissue versus heterologous tissue when used in selected anatomic locations. 2,[4][5][6][7][8][9][10][11] The homologous ECM can preferentially maintain tissuespecific cell phenotypes, [4][5][6][7] promote cell proliferation, 6,8 induce tissue-specific differentiation, 9 and enhance the chemotaxis of stem cells. 2,10,12 However, the preference or necessity for tissue-specific ECM has not been shown for all therapeutic applications.…”

mentioning

confidence: 99%