Tissue‐specific expression of <i>barnase</i> in tobacco delays axillary shoot development after topping

Grundmann, Lena; Känel, Andrea; Muth, Jost; Beinecke, Farina; Jekat, Marion; Shen, Yanxin; Chengalrayan, K.; Xu, Dongmei; Yang, Jingjing; Warek, Ujwala; Strickland, James A.; Prüfer, Dirk; Noll, Gundula A.

doi:10.1111/pbi.13759

Cited by 4 publications

(2 citation statements)

References 6 publications

(4 reference statements)

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“…Topping, the decapitation or removal of the apical bud, is necessary to enhance tobacco leaf development or maturation before harvesting. However, axillary shoot outgrowth induced by topping is undesirable since it reallocates resources to axillary buds, reducing the yield and quality of the main leaves 2 – 5 . Axillary shoot outgrowth can be inhibited by manually removing the suckers and applying fatty alcohols, flumetralin, or maleic hydrazide.…”

Section: Introductionmentioning

confidence: 99%

Integrative proteome and metabolome unveil the central role of IAA alteration in axillary bud development following topping in tobacco

Zou,

Zhang,

Liu

et al. 2024

Sci Rep

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Axillary bud is an important aspect of plant morphology, contributing to the final tobacco yield. However, the mechanisms of axillary bud development in tobacco remain largely unknown. To investigate this aspect of tobacco biology, the metabolome and proteome of the axillary buds before and after topping were compared. A total of 569 metabolites were differentially abundant before and 1, 3, and 5 days after topping. KEGG analyses further revealed that the axillary bud was characterized by a striking enrichment of metabolites involved in flavonoid metabolism, suggesting a strong flavonoid biosynthesis activity in the tobacco axillary bud after topping. Additionally, 9035 differentially expressed proteins (DEPs) were identified before and 1, 3, and 5 days after topping. Subsequent GO and KEGG analyses revealed that the DEPs in the axillary bud were enriched in oxidative stress, hormone signal transduction, MAPK signaling pathway, and starch and sucrose metabolism. The integrated proteome and metabolome analysis revealed that the indole-3-acetic acid (IAA) alteration in buds control dormancy release and sustained growth of axillary bud by regulating proteins involved in carbohydrate metabolism, amino acid metabolism, and lipid metabolism. Notably, the proteins related to reactive oxygen species (ROS) scavenging and flavonoid biosynthesis were strongly negatively correlated with IAA content. These findings shed light on a critical role of IAA alteration in regulating axillary bud outgrowth, and implied a potential crosstalk among IAA alteration, ROS homeostasis, and flavonoid biosynthesis in tobacco axillary bud under topping stress, which could improve our understanding of the IAA alteration in axillary bud as an important regulator of axillary bud development.

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Section: Introductionmentioning

confidence: 99%

Integrative proteome and metabolome unveil the central role of IAA alteration in axillary bud development following topping in tobacco

Zou,

Zhang,

Liu

et al. 2024

Sci Rep

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show abstract

“…Transcription factors regulate gene expression by binding to various cis-regulatory elements in the promoter. Currently, the most extensively studied promoters in plants are constitutive promoters, [12][13][14][15][16] tissue-specific promoters, 11,[17][18][19][20] and abiotic stress-inducible promoters. [21][22][23][24][25] Among stressinducible promoters, pathogen-inducible promoters are more often studied.…”

Section: Introductionmentioning

confidence: 99%

Cloning of the promoter of rice brown planthopper feeding‐inducible gene OsTPS31 and identification of related cis‐regulatory elements

Chang

Yang

et al. 2023

Pest Management Science

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Background: Brown planthopper (BPH; Nilaparvata lugens) is one of the most serious pests of rice in the world. Insect-resistant genetic engineering is a very effective technology to control BPH. The promoters and cis-regulatory elements inducible by plant-feeding insects are critical for genetic engineering of insect-resistant crops.Results: In this study, we cloned a promoter Ptps31 and a 7 bp cis-regulatory sequence that up-regulated downstream genes induced by BPH feeding. The promoter of OsTPS31 (Ptps31) unresponsive to physical damage but responsive to BPH feeding was cloned and functionally verified. The results showed that expression of the OsBPH14 gene driven by the promoter region from −510 to −246 bp in rice could significantly improve the resistance to BPH. The promoter region from −376 to −370 bp (TAGTGTC) was identified as a cis-regulatory sequence related to BPH feeding induction of downstream gene expression. Conclusion:The findings provide a new promoter and a new cis-regulatory sequence tool for the research on and application of rice BPH resistance genes, as well as a new perspective for functional analysis of the OsTPS31 gene.

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The role of strigolactone analog (GR24) in endogenous hormone metabolism and hormone-related gene expression in tobacco axillary buds

Tian,

Tang,

Fan

et al. 2023

Plant Cell Rep

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Tissue‐specific expression of barnase in tobacco delays axillary shoot development after topping

Cited by 4 publications

References 6 publications

Integrative proteome and metabolome unveil the central role of IAA alteration in axillary bud development following topping in tobacco

Integrative proteome and metabolome unveil the central role of IAA alteration in axillary bud development following topping in tobacco

Cloning of the promoter of rice brown planthopper feeding‐inducible gene OsTPS31 and identification of related cis‐regulatory elements

The role of strigolactone analog (GR24) in endogenous hormone metabolism and hormone-related gene expression in tobacco axillary buds

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