Tissue-Specific Expression of Germin-Like Oxalate Oxidase during Development and Fungal Infection of Barley Seedlings

Dumas, Bernard; Freyssinet, Georges; Pallett, Ken

doi:10.1104/pp.107.4.1091

Cited by 165 publications

(117 citation statements)

References 28 publications

(30 reference statements)

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“…Their hydrogen peroxide (H 2 O 2 )-generating capacity together with their mRNA accumulation patterns and apoplastic location are consistent with a role in cell wall extensibility, restriction of organ growth, and lignification during plant development and defense (Lane 1994). Cell wall modification during development (e.g., germination) or pathogen infection often requires the crosslinking of cell wall polymers (Carpita and Gibeaut 1993;Dumas et al 1995;Lane 1994Lane , 2002. Germins might mediate cell wall modifications by locally generating the H 2 O 2 required for such reactions (Lane 1994).…”

Section: Discussionmentioning

confidence: 70%

Spatial and Temporal Divergence of Expression in Duplicated Barley Germin-Like Protein-Encoding Genes

et al. 2006

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Subfunctionalization is the process by which a pair of duplicated genes, or paralogs, experiences a reduction of individual expression patterns or function while still reproducing the complete expression pattern and function of the ancestral gene. Two germin-like protein (GLP)-encoding genes, GerB and GerF, are paralogs that belong to a small gene family in barley (Hordeum vulgare). Both genes share high nucleotide sequence similarity in coding and noncoding regions and encode identical apoplastic proteins. The use of RNA gel blots, coupled with single-stranded conformation polymorphism (SSCP) analysis of RT–PCR products, elucidated the developmental and tissue-specific expression patterns of each gene. Individual expression patterns provided evidence of both overlapping redundancy and early subfunctionalization. GerB is predominantly expressed in developing shoots, while GerF is predominantly expressed in seedling roots, developing spikes, and pericarp/testa. GerF promoter deletion studies located a region (−356/−97) responsible for high promoter activity and showed the ability of GerB and GerF upstream regions to drive gfp expression in coleoptiles, epicarps, and lemma/palea of developing spikes. The observed expression patterns are consistent with proposed roles in plant development and defense mechanisms for this gene family. These roles may explain why redundancy has been selectively maintained in this duplicate gene pair.

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Section: Discussionmentioning

confidence: 70%

Spatial and Temporal Divergence of Expression in Duplicated Barley Germin-Like Protein-Encoding Genes

et al. 2006

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“…This activity utilizes oxalic acid to generate hydrogen peroxide. In this study, we localized germin enzymatic activity by a method which was explained by Dumas et al (1995). The dark paint which was an indication of germin enzymatic activity was associated with almost all of the coleoptile cells; however, it was more conspicuous with the outer epidermis (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Histochemical detection of oxalate oxidase activity Oxalate oxidase activity in situ was detected using the procedure of Dumas et al (1995). Germinated wheat embryo coleoptile was dissected and sectioned at −20 o C after freeze-embedding in OCT resin: plant material was fixed in 4% paraformaldehyde in phosphate buffered saline (PBS), then washed with PBS, prior to embedding.…”

Section: Methodsmentioning

confidence: 99%

Localization of Germin Genes and Their Products in Developing Wheat Coleoptiles

Çalışkan¹,

Özcan²,

Turan³

et al. 2004

BMB Reports

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Germination is a process which characterized with nescient synthesis of genes. Among the genes synthesized during the germination of wheat embryos, germin genes, proteins and their enzymatic activity were defined. Germin is a water soluble homopentameric glycoprotein which is remarkable resistant to degradation by a broad range of proteases including pepsin. Germin proteins found to have strong oxalate oxidase activity which produces hydrogen peroxide by degrading oxalic acid. The current study, aimed to localize the germin genes, proteins and enzymatic activities in developing coleoptiles which is a rapidly growing protective tissue of leaf primordium and shoot apex. Non-radioactively labeled germin riboprobes were employed to localize germin mRNAs in situ. FITC (Fluorescein isothiocyanate) and alkaline phosphatase linked anti-germin antibodies were used to localize germin proteins under the fluorescence and light microscopy and finally germin enzymatic activity was localized by using appropriate enzyme assay. The results revealed that in coleoptiles germin genes, proteins and their enzymatic activity were predominantly associated with the cells of epidermis and vascular bundle sheath cells.

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“…Numerous functions have been proposed for GLPs, including desiccation and hydration (39), restructuring of cell walls (13), salt and heavy metal response (40), and plant defenses (15,41,42). Because GLPs represent a large family of extracellular proteins, some of which have superoxide dismutase activity, we propose a novel function for these proteins in mediating the oxidative burst during the wound response.…”

Section: Discussionmentioning

confidence: 97%

Tobacco Nectarin I

Carter

Thornburg

2000

Journal of Biological Chemistry

193

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Nectarin I, a protein that accumulates in the nectar of Nicotiana sp., was determined to contain superoxide dismutase activity by colorimetric and in-gel assays. This activity was found to be remarkably thermostable. did not restore superoxide dismutase activity. The quaternary structure of the reconstituted enzyme was examined, and only tetrameric and pentameric aggregates were enzymatically active. The reconstituted enzyme was also shown to generate H 2 O 2 . Putative nectarin I homologues were found in the nectars of several other plant species.

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Tissue-Specific Expression of Germin-Like Oxalate Oxidase during Development and Fungal Infection of Barley Seedlings

Cited by 165 publications

References 28 publications

Spatial and Temporal Divergence of Expression in Duplicated Barley Germin-Like Protein-Encoding Genes

Spatial and Temporal Divergence of Expression in Duplicated Barley Germin-Like Protein-Encoding Genes

Localization of Germin Genes and Their Products in Developing Wheat Coleoptiles

Tobacco Nectarin I

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