Tissue specific differences in the 2′‐<i>O</i>‐methylation of eukaryotic 5.8S ribosomal RNA

Nazar, Ross N.; Sitz, Thomas O.; Busch, Harris

doi:10.1016/0014-5793(75)80346-2

Cited by 40 publications

(59 citation statements)

References 11 publications

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“…It is encoded by the major ribosomal DNA (rDNA) ' and is co-transcribed with 18 S and 28 S RNA within ribosomal precursor RNA 5,6. It is cleaved into a separate entity during ribosome maturation and remains noncovalently attached to 28 S RNA1'.…”

Section: Introductionmentioning

confidence: 99%

Nucleotide sequence relationships between vertebrate 5.8 S ribosomal RNAs

Khan

Maden

1977

Nucl Acids Res

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Nucleotide sequences of the 5.8 S ribosomal RNAs from HeLa cells, Xenopus laevis and chick embryo fibroblasts were compared. Xenopus laevis 5.8 S RNA differs from that of HeLa cells in four internal positions and at the 3' end of the molecule.Chick 5.8 S RNA differs from that of HeLa cells in two positions.Six out of the seven interspecies differences are due to base substitutions.The other difference is due to the presence of an extra nucleotide, internally located, within the Xenopus 5.8 S sequence.

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Section: Introductionmentioning

confidence: 99%

Nucleotide sequence relationships between vertebrate 5.8 S ribosomal RNAs

Khan

Maden

1977

Nucl Acids Res

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“…2, pointers) were found in the in vitro transcripts and not in the 45S pre-rRNA. It is possible that the missing fragments were undermethylated (19) and accordingly were cleaved by T, RNase.…”

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confidence: 99%

Fidelity of synthesis of preribosomal RNA in isolated nucleoli and nucleolar chromatin.

Ballal¹,

Youngik²,

Mouche³

et al. 1977

Proc. Natl. Acad. Sci. U.S.A.

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Previously, other workers have shown that isolated nucleoli can synthesize RNA in vitro that resembles pre-rRNA in base composition, marker oligonucleotides, and hybridization analysis (4-10). The present study further supports the fidelity of pre-rRNA synthesis by isolated nucleoli and nucleolar chromatin, using the homochromatographic fingerprinting technique, which permits a direct comparison of patterns of virtually all the oligonucleotides obtained by T1 ribonuclease digestion of 45S pre-rRNA.Abbreviation: pre-rRNA, preribosomal RNA. MATERIALS AND METHODSIsolation of Nucleoli and Nucleolar Chromatin. Nucleoli were isolated from 6-day-old Novikoff hepatoma ascites cells (11). They were suspended in 0.05 M Tris-HCI, pH 7.9/50% glycerol/i mM MgCl2/0.1 mM EDTA/1 mM dithiothreitol. RNA Synthesis In Vitro. RNA was synthesized in a standard system (6, 12). Similar conditions were used for assays with nucleolar chromatin template and solubilized polymerase I except that 5 mM MgCl2 was replaced by 2.5 mM MgCl2 and 2 mM MnCI2.When the in vitro transcripts were to be analyzed by ho-

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“…Free 5.8 S rRNA, 60 S subunits and whole ribosomes were treated with diethyl pyrocarbonate as in section 2, reactive sites were detected by subsequent aniline degradation and analyses on 12% polyacrylamide gels as shown in fig.1. The nucleotide sequence was determined in [18] and the secondary structure is based on the 'burp gun' model [19]. Large arrows and small arrowheads indicate strongly or weakly reactive sites, respectively, in 60 S subunits and whole ribosomes; the shaded areas indicate residues in free 5.8 S RNA which were essentially unreactive.…”

Section: Resultsmentioning

confidence: 99%

Tissue specific differences in the 2′‐O‐methylation of eukaryotic 5.8S ribosomal RNA

Cited by 40 publications

References 11 publications

Nucleotide sequence relationships between vertebrate 5.8 S ribosomal RNAs

Nucleotide sequence relationships between vertebrate 5.8 S ribosomal RNAs

Fidelity of synthesis of preribosomal RNA in isolated nucleoli and nucleolar chromatin.

Use of diethyl pyrocarbonate reactivity as a probe for the topography of 5.8 S rRNA in yeast ribosomes

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