Tissue Recombination Models for the Study of Epithelial Cancer

Zong, Yang; Goldstein, Andrew S.; Witte, Owen N.

doi:10.1101/pdb.top069880

Cited by 3 publications

(3 citation statements)

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“…Much current understanding is drawn from experiments using mutant mice or immortalized cell lines or from investigation of established cancers in all of which models tumors must achieve a certain mass to be detected and hence to be sampled. In no model can one glimpse the moment a normal cell becomes a transformed cell or sample cells at the inception of cancer [ 67 , 68 , 69 , 70 ]. For this reason, there has been great enthusiasm (although much controversy as well) for validating the fundamental concepts of oncogenesis derived from experimental models and from analysis of tumor samples with cancer epidemiology [ 71 , 72 , 73 , 74 , 75 , 76 ].…”

Section: Reflections On the Role Of Cell Fusion In Oncogenesis Andmentioning

confidence: 99%

Cell Fusion in the War on Cancer: A Perspective on the Inception of Malignancy

Platt

Zhou

Lefferts

et al. 2016

IJMS

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Cell fusion occurs in development and in physiology and rarely in those settings is it associated with malignancy. However, deliberate fusion of cells and possibly untoward fusion of cells not suitably poised can eventuate in aneuploidy, DNA damage and malignant transformation. How often cell fusion may initiate malignancy is unknown. However, cell fusion could explain the high frequency of cancers in tissues with low underlying rates of cell proliferation and mutation. On the other hand, cell fusion might also engage innate and adaptive immune surveillance, thus helping to eliminate or retard malignancies. Here we consider whether and how cell fusion might weigh on the overall burden of cancer in modern societies.

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Section: Reflections On the Role Of Cell Fusion In Oncogenesis Andmentioning

confidence: 99%

Cell Fusion in the War on Cancer: A Perspective on the Inception of Malignancy

Platt

Zhou

Lefferts

et al. 2016

IJMS

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“…While organoid culturing has the advantage of being able to manipulate single (ie, stromal) factors to assess their effect on tumor growth, this ex vivo approach has limitations. In vivo tissue recombination approaches could provide a more physiological context that better mimics the prostate microenvironment . Human prostatic epithelial cells can be recombined with human prostatic stromal cells and grafted under the renal capsules of mice.…”

Section: Microenvironment: the Cancer Swampsmentioning

confidence: 99%

Tumor cell heterogeneity and resistance; report from the 2018 Coffey‐Holden Prostate Cancer Academy Meeting

Miyahira

Den²,

Carlo³

et al. 2018

The Prostate

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Introduction The 2018 Coffey‐Holden Prostate Cancer Academy (CHPCA) Meeting, “Tumor Cell Heterogeneity and Resistance,” was held in Los Angeles, California from June 21 to 24, 2018. Methods The CHPCA Meeting is a unique, discussion‐oriented scientific conference convened annually by the Prostate Cancer Foundation (PCF), which focuses on the most critical topics in need of further study to advance the treatment of lethal prostate cancer. The 6th Annual CHPCA Meeting was attended by 70 investigators and concentrated on prostate cancer heterogeneity and treatment resistance. Results The meeting focused on topics including: recognition of tumor heterogeneity, molecular drivers of heterogeneity, the role of the tumor microenvironment, the role of heterogeneity in disease progression, metastasis and treatment resistance, clinical trials designed to target resistance and tumor heterogeneity, and immunotherapeutic approaches to target and overcome tumor heterogeneity. Discussion This review article summarizes the presentations and discussions from the 2018 CHPCA Meeting in order to share this knowledge with the scientific community and encourage new studies that will lead to improved treatments and outcomes for men with prostate cancer.

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“…Cleared MFP transplantation was first showed in the pioneering work of DeOme et al (1959); for more information, see Introduction: Tissue Recombination Models for the Study of Epithelial Cancer (Zong et al 2014). …”

Section: Related Informationmentioning

confidence: 99%

The Cleared Mammary Fat Pad Transplantation Assay for Mammary Epithelial Organogenesis

Lawson

Werb

Zong

et al. 2015

Cold Spring Harb Protoc

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Cleared mammary fat pad (MFP) transplantation has been a standard technique for studies of mammary development and cancer for several decades. The mammary gland is comprised of several fundamental components: The epithelial compartment contains basal/myoepithelial cells and luminal cells, and the stromal compartment (called the MFP) contains adipocytes, smooth muscle cells, fibroblasts, and immune cells. In 3- to 4-wk-old female mice, the mammary epithelium is concentrated very close to the nipple and has not yet grown beyond the mammary lymph node to penetrate the bulk of the MFP. This developmental feature provides an anatomical fixed point, and enables one to cut away the portion of the MFP from the nipple to the lymph node, leaving behind the majority of the MFP free of epithelium. The “cleared” MFP can serve as a supportive native micro-environment fully sufficient for the organogenesis of injected donor epithelium. Normal mammary epithelial donor cells will produce histologically and functionally normal mammary ductal epithelium several weeks posttransplant, with the exception that the ducts will not be connected to the nipple. The assay described here provides a powerful platform for assessing the developmental and tumorigenic potential of engineered cells of interest.

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Tissue Recombination Models for the Study of Epithelial Cancer

Cited by 3 publications

References 100 publications

Cell Fusion in the War on Cancer: A Perspective on the Inception of Malignancy

Cell Fusion in the War on Cancer: A Perspective on the Inception of Malignancy

Tumor cell heterogeneity and resistance; report from the 2018 Coffey‐Holden Prostate Cancer Academy Meeting

The Cleared Mammary Fat Pad Transplantation Assay for Mammary Epithelial Organogenesis

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