Tissue Polarimetry

Vitkin, I. Alex; Ghosh, Nirmalya; Martino, A. De

doi:10.1002/9781119011804.ch7

Cited by 56 publications

(64 citation statements)

References 104 publications

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“…The transformation of an arbitrary polarized light (linear, circular, or elliptical) by a scattering particle can be described using a linear relationship between the incident and the scattered field components, 5,15,22,[30][31][32][33][34][35][36][37][38][96][97][98][99][100][101] E Q -T A R G E T ; t e m p : i n t r a l i n k -; e 0 0 1 ; 6 3 ; 3 0 1…”

Section: Fundamentals Of Polarized Light Scatteringmentioning

confidence: 99%

Polarized light interaction with tissues

2016

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This tutorial-review introduces the fundamentals of polarized light interaction with biological tissues and presents some of the recent key polarization optical methods that have made possible the quantitative studies essential for biomedical diagnostics. Tissue structures and the corresponding models showing linear and circular birefringence, dichroism, and chirality are analyzed. As the basis for a quantitative description of the interaction of polarized light with tissues, the theory of polarization transfer in a random medium is used. This theory employs the modified transfer equation for Stokes parameters to predict the polarization properties of single- and multiple-scattered optical fields. The near-order of scatterers in tissues is accounted for to provide an adequate description of tissue polarization properties. Biomedical diagnostic techniques based on polarized light detection, including polarization imaging and spectroscopy, amplitude and intensity light scattering matrix measurements, and polarization-sensitive optical coherence tomography are described. Examples of biomedical applications of these techniques for early diagnostics of cataracts, detection of precancer, and prediction of skin disease are presented. The substantial reduction of light scattering multiplicity at tissue optical clearing that leads to a lesser influence of scattering on the measured intrinsic polarization properties of the tissue and allows for more precise quantification of these properties is demonstrated.

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Section: Fundamentals Of Polarized Light Scatteringmentioning

confidence: 99%

Polarized light interaction with tissues

2016

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“…This method may have advantages when investigating depolarizing media with tilted input and output interfaces in which case interface refraction induced diattenuation and retardance match with the equivalent cascade of five elements [29]. It is noted that the symmetric decomposition cannot be applied for a special class of Mueller matrices known as non-Stokes diagonalizable Mueller matrices [117,140,141].…”

Section: Factor Product Decompositionmentioning

confidence: 99%

“…Additional care should be taken in practice when they are used with coherent light sources that may result in speckles [10,[26][27][28]. For example, the size of the detector used for Stokes polarimetry should normally be large enough to cover a sufficient number of speckles, that is, the detection area should be much larger than a typical speckle size, in order to determine the depolarization property of a turbid media [26,29].…”

Section: Stokes Parameters and Mueller Matricesmentioning

confidence: 99%

Mueller polarimetric imaging for surgical and diagnostic applications: a review

2017

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Polarization is a fundamental property of light and a powerful sensing tool that has been applied to many areas. A Mueller matrix is a complete mathematical description of the polarization characteristics of objects that interact with light, and is known as a transfer function of Stokes vectors which characterise the state of polarization of light. Mueller polarimetric imaging measures Mueller matrices over a field of view and thus allows for visualising the polarization characteristics of the objects. It has emerged as a promising technique in recent years for tissue imaging, improving image contrast and providing a unique perspective to reveal additional information that cannot be resolved by other optical imaging modalities. This review introduces the basis of the Stokes-Mueller formulism, interpretation methods of Mueller matrices into fundamental polarization properties, polarization properties of biological tissues, and considerations in the construction of Mueller polarimetric imaging devices for surgical and diagnostic applications, including primary configurations, optimization procedures, calibration methods as well as the instrument polarization properties of several widelyused biomedical optical devices. The paper also reviews recent progress in Mueller polarimetric endoscopes and fibre Mueller polarimeters, followed by the future outlook in applying the technique to surgery and diagnostics. Tissue polarization properties convey morphological, micro-structural and compositional information of tissue with great potential for label free characterization of tissue pathological changes. Recent progress in tissue polarimetric imaging and polarization resolved endoscopy paved the way for translation of polarimetric imaging to surgery and tissue diagnosis.

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“…1 It has been shown that Mueller matrix formalism is a powerful tool in the study of biological samples. [2][3][4] This phenomenological approach describes the interaction of polarized light with a sample using a model of "equivalent optical circuit" built from the basic optical elements-diatenuators, retarders, and depolarizers. The extended toolkit of various Mueller matrix decompositions (data processing algorithms exploring nonlinear compression of a set of real values of 4 × 4 Mueller matrices) is available for data analysis and characterization of tissue optical properties.…”

Section: Introductionmentioning

confidence: 99%

Analysis of tissue microstructure with Mueller microscopy: logarithmic decomposition and Monte Carlo modeling

Lee

Chandel

et al. 2020

J. Biomed. Opt.

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Significance: Definitive diagnostics of many diseases is based on the histological analysis of thin tissue cuts with optical white light microscopy. Extra information on tissue structural properties obtained with polarized light would help the pathologist to improve the accuracy of his diagnosis.Aim: We report on using Mueller matrix microscopy data, logarithmic decomposition, and polarized Monte Carlo (MC) modeling for qualitative and quantitative analysis of thin tissue cuts to extract the information on tissue microstructure that is not available with a conventional white light microscopy.Approach: Unstained cuts of human skin equivalents were measured with a custom-built liquidcrystal-based Mueller microscope in transmission configuration. To interpret experimental data, we performed the simulations with a polarized MC algorithm for scattering anisotropic media. Several optical models of tissue (spherical scatterers within birefringent host medium, and combination of spherical and cylindrical scatterers within either isotropic or birefringent host medium) were tested.Results: A set of rotation invariants for the logarithmic decomposition of a Mueller matrix was derived to rule out the impact of sample orientation. These invariants were calculated for both simulated and measured Mueller matrices of the dermal layer of skin equivalents. We demonstrated that only the simulations with a model combining both spherical and cylindrical scatterers within birefringent host medium reproduced the experimental trends in optical properties of the dermal layer (linear retardance, linear dichroism, and anisotropic linear depolarization) with layer thickness.Conclusions: Our studies prove that Mueller polarimetry provides relevant information not only on a size of dominant scatterers (e.g., cell nuclei versus subwavelength organelles) but also on its shape (e.g., cells versus collagen fibers). The latter is directly related to the state of extracellular collagen matrix, which is often affected by early pathology. Hence, using polarimetric data can help to increase the accuracy of diagnosis.

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Tissue Polarimetry

Cited by 56 publications

References 104 publications

Polarized light interaction with tissues

Polarized light interaction with tissues

Mueller polarimetric imaging for surgical and diagnostic applications: a review

Analysis of tissue microstructure with Mueller microscopy: logarithmic decomposition and Monte Carlo modeling

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