Tissue Organoids: Liver

Solanas, E.; Plá-Palacín, Iris; Sainz‐Arnal, Pilar; Almeida, Manuel; Lué, Alberto; Serrano, Trinidad; Baptista, Pedro M.

doi:10.1007/978-3-319-60511-1_2

Cited by 1 publication

(1 citation statement)

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“…To date, protocols of directed differentiation to obtain cholangiocytes[56,57], endothelia cells[60] and hepatic stellate cells[61] have been established, which may further aid the generation of functional liver tissue organoids. Other reviews discuss the generation and application of tissue organoids, which can assist in better understanding the opportunities as well as challenges in this field[62,63].…”

Section: Applications Of Hlcsmentioning

confidence: 99%

Derivation and applications of human hepatocyte-like cells

Li¹,

Huang²,

Zhao³

et al. 2019

WJSC

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Human hepatocyte-like cells (HLCs) derived from human pluripotent stem cells (hPSCs) promise a valuable source of cells with human genetic background, physiologically relevant liver functions, and unlimited supply. With over 10 years’ efforts in this field, great achievements have been made. HLCs have been successfully derived and applied in disease modeling, toxicity testing and drug discovery. Large cohorts of induced pluripotent stem cells-derived HLCs have been recently applied in studying population genetics and functional outputs of common genetic variants in vitro . This has offered a new paradigm for genome-wide association studies and possibly in vitro pharmacogenomics in the nearly future. However, HLCs have not yet been successfully applied in bioartificial liver devices and have only displayed limited success in cell transplantation. HLCs still have an immature hepatocyte phenotype and exist as a population with great heterogeneity, and HLCs derived from different hPSC lines display variable differentiation efficiency. Therefore, continuous improvement to the quality of HLCs, deeper investigation of relevant biological processes, and proper adaptation of recent advances in cell culture platforms, genome editing technology, and bioengineering systems are required before HLCs can fulfill the needs in basic and translational research. In this review, we summarize the discoveries, achievements, and challenges in the derivation and applications of HLCs.

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